Characterization of WWOX inactivation in murine mammary gland development

2013 ◽  
Vol 228 (7) ◽  
pp. 1391-1396 ◽  
Author(s):  
Suhaib K. Abdeen ◽  
Zaidoun Salah ◽  
Saleh Khawaled ◽  
Rami I. Aqeilan
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Gland Development

Characterization of mammary gland development in pregnant gilts1

2006 ◽  
Vol 84 (3) ◽  
pp. 579-587 ◽  
Author(s):  
F. Ji ◽  
W. L. Hurley ◽  
S. W. Kim
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Gland Development

scholarly journals Identification and partial characterization of a novel circular transcript of the Tc2n gene from rat mammary gland

2016 ◽  
Vol 68 (2) ◽  
pp. 257-262 ◽  
Author(s):  
Shi Zhu ◽  
YanHong Wang ◽  
Yu Zhao ◽  
Jia Chen ◽  
Chen Chen ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Expression Profile ◽  
Mammary Gland Development ◽  
Regulatory Mechanism ◽  
Circular Rna ◽  
Partial Characterization ◽  
Circular Rnas ◽  
Rat Mammary Gland ◽  
Gland Development

The regulatory mechanism of mammary gland development and lactation is important, as lactation not only provides healthy milk for infants, but also provides health benefits to mothers. Circular RNAs (circRNAs) have recently received much attention, but only a few have been functionally characterized. In the present study, the structure and expression profile of a rat Tc2n-derived circular RNA (cTc2n) was investigated by several protocols. The cTc2n comprised exons10 and 11 of the Tc2ngeneandhad no poly(A)structure. The expression of cTc2n was significantly higher in the mammary gland of rat than in other tissues, including the heart, liver, spleen, lung, kidney, uterus and ovary. The abundance of cTc2n was the highest on day 1, and significantly declined on day 7, slightly increasing on day 21 postpartum. This finding indicates that cTc2n might play an important role in lactation.

scholarly journals Characterization of RNA Editome in the Mammary Gland of Yaks during the Lactation and Dry Periods

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 207
Author(s):  
Xiaoyun Wu ◽  
Wondossen Ayalew ◽  
Min Chu ◽  
Jie Pei ◽  
Chunnian Liang ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Rna Editing ◽  
Mammary Gland Development ◽  
Lactation Period ◽  
Coding Regions ◽  
Significant Difference ◽  
Functional Consequences ◽  
First Time ◽  
Gland Development

The mammary gland is a complicated organ comprising several types of cells, and it undergoes extensive morphogenetic and metabolic changes during the female reproductive cycle. RNA editing is a posttranscriptional modification event occurring at the RNA nucleotide level, and it drives transcriptomic and proteomic diversities, with potential functional consequences. RNA editing in the mammary gland of yaks, however, remains poorly understood. Here, we used REDItools to identify RNA editing sites in mammary gland tissues in yaks during the lactation period (LP, n = 2) and dry period (DP, n = 3). Totally, 82,872 unique RNA editing sites were identified, most of which were detected in the noncoding regions with a low editing degree. In the coding regions (CDS), we detected 5235 editing sites, among which 1884 caused nonsynonymous amino acid changes. Of these RNA editing sites, 486 were found to generate novel possible miRNA target sites or interfere with the initial miRNA binding sites, indicating that RNA editing was related to gene regulation mediated by miRNA. A total of 14,159 RNA editing sites (involving 3238 common genes) showed a significant differential editing level in the LP when compared with that in the DP through Tukey’s Honest Significant Difference method (p < 0.05). According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, genes that showed different RNA editing levels mainly participated in pathways highly related to mammary gland development, including MAPK, PI3K-Akt, FoxO, and GnRH signaling pathways. Collectively, this work demonstrated for the first time the dynamic RNA editome profiles in the mammary gland of yaks and shed more light on the mechanism that regulates lactation together with mammary gland development.

scholarly journals Bovine mammary gland development: new insights into the epithelial hierarchy

2018 ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Stem Cell ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Mammary Epithelium ◽  
Prolactin Receptors ◽  
Bovine Mammary Gland ◽  
Mammary Stem ◽  
Gland Development ◽  
Developmental Hierarchy

AbstractMilk production is highly dependent on the extensive development of the mammary epithelium, which occurs during puberty. It is therefore essential to distinguish the epithelial cells committed to development during this key stage from the related epithelial hierarchy. Using cell phenotyping and sorting, we highlighted three sub-populations that we assume to be progenitors. The CD49fhighCD24neg cells expressing KRT14, vimentin and PROCR corresponded to basal progenitors whereas the CD49flowCD24neg cells expressing luminal KRT, progesterone and prolactin receptors, were of luminal lineage. The CD49flowCD24pos cells had features of a dual lineage, with luminal and basal characteristics (CD10, ALDH1 and KRT7 expression) and were considered to be early common (bipotent) progenitors. The mammary stem cell (MaSC) fraction was recovered in a fourth sub-population of CD49fhighCD24pos cells that expressed CD10/KRT14 and KRT7. The differential ALDH1 activities observed within the MaSC fraction allowed to discriminate between two states: quiescent MaSCs and lineage-restricted “activated” MaSCs. The in-depth characterization of these epithelial sub-populations provides new insights into the epithelial cell hierarchy in the bovine mammary gland and suggests a common developmental hierarchy in mammals.

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