Mouse embryonic stem cells that survive γ-rays exposure maintain pluripotent differentiation potential and genome stability

2011 ◽  
Vol 227 (3) ◽  
pp. 1242-1249 ◽  
Author(s):  
Paola Rebuzzini ◽  
Diana Pignalosa ◽  
Giuliano Mazzini ◽  
Riccardo Di Liberto ◽  
Antonio Coppola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Genome Stability ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Differentiation Potential ◽  
Γ Rays

scholarly journals Wnt Signaling Regulates the Lineage Differentiation Potential of Mouse Embryonic Stem Cells through Tcf3 Down-Regulation

PLoS Genetics ◽  
2013 ◽  
Vol 9 (5) ◽  
pp. e1003424 ◽  
Author(s):  
Yaser Atlasi ◽  
Rubina Noori ◽  
Claudia Gaspar ◽  
Patrick Franken ◽  
Andrea Sacchetti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Down Regulation ◽  
Differentiation Potential ◽  
Lineage Differentiation

scholarly journals Undersulfation of Heparan Sulfate Restricts Differentiation Potential of Mouse Embryonic Stem Cells

2012 ◽  
Vol 287 (14) ◽  
pp. 10853-10862 ◽  
Author(s):  
Maud Forsberg ◽  
Katarina Holmborn ◽  
Soumi Kundu ◽  
Anders Dagälv ◽  
Lena Kjellén ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Heparan Sulfate ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Differentiation Potential

Mathematical modeling of growth and death dynamics of mouse embryonic stem cells irradiated with γ-rays

2014 ◽  
Vol 363 ◽  
pp. 374-380 ◽  
Author(s):  
N. Terranova ◽  
P. Rebuzzini ◽  
G. Mazzini ◽  
E. Borella ◽  
C.A. Redi ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Γ Rays

Alteration of gene expression profile in mouse embryonic stem cells and neural differentiation deficits by ethephon

2020 ◽  
Vol 39 (11) ◽  
pp. 1518-1527
Author(s):  
S Mohammadi Nejad ◽  
M Hodjat ◽  
SA Mousavi ◽  
M Baeeri ◽  
MA Rezvanfar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Differentiation Potential

Ethephon, a member of the organophosphorus compounds, is one of the most widely used plant growth regulators for artificial ripening. Although million pounds of this chemical is being used annually, the knowledge regarding its molecular toxicity is yet not sufficient. The purpose of this study was to evaluate the potential developmental toxicity of ethephon using embryonic stem cell model. The mouse embryonic stem cells (mESCs) were exposed to various concentrations of ethephon and the viability, cell cycle alteration and changes in the gene expression profile were evaluated using high-throughput RNA sequencing. Further, the effect of ethephon on neural differentiation potential was examined. The results showed that ethephon at noncytotoxic doses induced cell cycle arrest in mESCs. Gene ontology enrichment analysis showed that terms related to cell fate and organismal development, including neuron fate commitment, embryo development and cardiac cell differentiation, were markedly enriched in ethephon-treated cells. Neural induction of mESCs in the presence of ethephon was inhibited and the expression of neural genes was decreased in differentiated cells. Results obtained from this work clearly demonstrate that ethephon affects the gene expression profile of undifferentiated mESCs and prevents neural differentiation. Therefore, more caution against the frequent application of ethephon is advised.

scholarly journals Tcea3 Regulates the Vascular Differentiation Potential of Mouse Embryonic Stem Cells

2013 ◽  
Vol 16 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Young Cha ◽  
Sun-Hee Heo ◽  
Hee-Jin Ahn ◽  
Seong Kyu Yang ◽  
Ji-Hwan Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Vascular Differentiation ◽  
Differentiation Potential

scholarly journals Mobilization of LINE-1 retrotransposons is restricted by Tex19.1 in mouse embryonic stem cells

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Marie MacLennan ◽  
Marta García-Cañadas ◽  
Judith Reichmann ◽  
Elena Khazina ◽  
Gabriele Wagner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Translational Regulation ◽  
Genome Stability ◽  
De Novo ◽  
Genetic Disorders ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Dna Hypomethylation ◽  
Genomic Locations

Mobilization of retrotransposons to new genomic locations is a significant driver of mammalian genome evolution, but these mutagenic events can also cause genetic disorders. In humans, retrotransposon mobilization is mediated primarily by proteins encoded by LINE-1 (L1) retrotransposons, which mobilize in pluripotent cells early in development. Here we show that TEX19.1, which is induced by developmentally programmed DNA hypomethylation, can directly interact with the L1-encoded protein L1-ORF1p, stimulate its polyubiquitylation and degradation, and restrict L1 mobilization. We also show that TEX19.1 likely acts, at least in part, through promoting the activity of the E3 ubiquitin ligase UBR2 towards L1-ORF1p. Moreover, loss of Tex19.1 increases L1-ORF1p levels and L1 mobilization in pluripotent mouse embryonic stem cells, implying that Tex19.1 prevents de novo retrotransposition in the pluripotent phase of the germline cycle. These data show that post-translational regulation of L1 retrotransposons plays a key role in maintaining trans-generational genome stability in mammals.

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