Decorin and chondroitin-6 sulfate inhibit B16V melanoma cell migration and invasion by cellular acidification

2011 ◽  
Vol 226 (10) ◽  
pp. 2641-2650 ◽  
Author(s):  
Christian Stock ◽  
Oliver Jungmann ◽  
Daniela G. Seidler
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
Cellular Acidification

scholarly journals Bornyl cis-4-Hydroxycinnamate Suppresses Cell Metastasis of Melanoma through FAK/PI3K/Akt/mTOR and MAPK Signaling Pathways and Inhibition of the Epithelial-to-Mesenchymal Transition

2018 ◽  
Vol 19 (8) ◽  
pp. 2152 ◽  
Author(s):  
Tzu-Yen Yang ◽  
Mei-Li Wu ◽  
Chi-I Chang ◽  
Chih-I Liu ◽  
Te-Chih Cheng ◽  
...  
Keyword(s):  
Cell Migration ◽  
Signaling Pathways ◽  
Melanoma Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Melanoma Cells ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Cell Migration And Invasion ◽  
Cell Metastasis ◽  
A375 Melanoma

Bornyl cis-4-hydroxycinnamate, a bioactive compound isolated from Piper betle stems, has the potential for use as an anti-cancer agent. This study investigated the effects of bornyl cis-4-hydroxycinnamate on cell migration and invasion in melanoma cells. Cell migration and invasion were compared in A2058 and A375 melanoma cell lines treated with/without bornyl cis-4-hydroxycinnamate (1–6 µM). To examine whether bornyl cis-4-hydroxycinnamate has a potential anti-metastatic effect on melanoma cells, cell migration and invasion assays were performed using a Boyden chamber assay and a transwell chamber in A2058 and A375 cells. Gelatin zymography was employed to determine the enzyme activities of MMP-2 and MMP-9. Cell lysates were collected for Western blotting analysis of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitors of metalloproteinase-1/2 (TIMP-1/2), as well as key molecules in the mitogen-activated protein kinase (MAPK), focal adhesion kinase (FAK)/ phosphatidylinositide-3 kinases (PI3K)/Akt/ mammalian target of rapamycin (mTOR), growth factor receptor-bound protein 2 (GRB2) signaling pathways. Our results demonstrated that bornyl cis-4-hydroxycinnamate is a potentially useful agent that inhibits melanoma cell migration and invasion, and altered melanoma cell metastasis by reducing MMP-2 and MMP-9 expression through inhibition of the FAK/PI3K/Akt/mTOR, MAPK, and GRB2 signaling pathways. Moreover, bornyl cis-4-hydroxycinnamate inhibited the process of the epithelial-to-mesenchymal transition in A2058 and A375 melanoma cells. These findings suggested that bornyl cis-4-hydroxycinnamate has potential as a chemotherapeutic agent, and warrants further investigation for its use in the management of human melanoma.

scholarly journals Ligand-dependent Inhibition of B16 Melanoma Cell Migration and Invasion via Endogenous S1P2G Protein-coupled Receptor

2003 ◽  
Vol 278 (35) ◽  
pp. 32841-32851 ◽  
Author(s):  
Kayo Arikawa ◽  
Noriko Takuwa ◽  
Hironori Yamaguchi ◽  
Naotoshi Sugimoto ◽  
Joji Kitayama ◽  
...  
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
B16 Melanoma ◽  
Migration And Invasion ◽  
Cell Migration And Invasion ◽  
B16 Melanoma Cell ◽  
Dependent Inhibition

scholarly journals Quercetin inhibits HGF/c-Met signaling and HGF-stimulated melanoma cell migration and invasion

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Hui-Hui Cao ◽  
Chi-Yan Cheng ◽  
Tao Su ◽  
Xiu-Qiong Fu ◽  
Hui Guo ◽  
...  
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
Migration And Invasion ◽  
Cell Migration And Invasion

Abstract 1642: The role of αv integrin in melanoma cell migration and invasion.

2013 ◽  
Author(s):  
Sangeet Lal ◽  
Y. Jeffrey Wu ◽  
Leslie L. Muldoon ◽  
Edward A. Neuwelt
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
Migration And Invasion ◽  
Cell Migration And Invasion

Abstract 1277: Neural crest-like gene FOXD1 plays a role in melanoma cell migration and invasion

2016 ◽  
Author(s):  
Huizi Wu ◽  
Lionel Larribere ◽  
Kasia Weina ◽  
Nathalie Knappe ◽  
Christoffer Gebhardt ◽  
...  
Keyword(s):  
Cell Migration ◽  
Neural Crest ◽  
Melanoma Cell ◽  
Migration And Invasion ◽  
Cell Migration And Invasion

miRNA-7-5p inhibits melanoma cell migration and invasion

2013 ◽  
Vol 430 (2) ◽  
pp. 706-710 ◽  
Author(s):  
Keith M. Giles ◽  
Rikki A.M. Brown ◽  
Michael R. Epis ◽  
Felicity C. Kalinowski ◽  
Peter J. Leedman
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
Migration And Invasion ◽  
Cell Migration And Invasion

scholarly journals Inhibition of Melanoma Cell Migration and Invasion Targeting the Hypoxic Tumor Associated CAXII

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 3018
Author(s):  
Gaia Giuntini ◽  
Sara Monaci ◽  
Ylenia Cau ◽  
Mattia Mori ◽  
Antonella Naldini ◽  
...  
Keyword(s):  
Cell Migration ◽  
Malignant Melanoma ◽  
Cell Motility ◽  
Cell Lines ◽  
Cancer Progression ◽  
Melanoma Cell ◽  
Migration And Invasion ◽  
Carbonic Anhydrases ◽  
Cell Migration And Invasion ◽  
Melanoma Cell Lines

Background: Intratumoral hypoxia contributes to cancer progression and poor prognosis. Carbonic anhydrases IX (CAIX) and XII (CAXII) play pivotal roles in tumor cell adaptation and survival, as aberrant Hedgehog (Hh) pathway does. In malignant melanoma both features have been investigated for years, but they have not been correlated before and/or identified as a potential pharmacological target. Here, for the first time, we demonstrated that malignant melanoma cell motility was impaired by targeting CAXII via either CAs inhibitors or through the inhibition of the Hh pathway. Methods: We tested cell motility in three melanoma cell lines (WM-35, SK-MEL28, and A375), with different invasiveness capabilities. To this end we performed a scratch assay in the presence of the smoothened (SMO) antagonist cyclopamine (cyclo) or CAs inhibitors under normoxia or hypoxia. Then, we analyzed the invasiveness potential in the cell lines which were more affected by cyclo and CAs inhibitors (SK-MEL28 and A375). Western blot was employed to assess the expression of the hypoxia inducible factor 1α, CAXII, and FAK phosphorylation. Immunofluorescence staining was performed to verify the blockade of CAXII expression. Results: Hh inhibition reduced melanoma cell migration and CAXII expression under both normoxic and hypoxic conditions. Interestingly, basal CAXII expression was higher in the two more aggressive melanoma cell lines. Finally, a direct CAXII blockade impaired melanoma cell migration and invasion under hypoxia. This was associated with a decrease of FAK phosphorylation and metalloprotease activities. Conclusions: CAXII may be used as a target for melanoma treatment not only through its direct inhibition, but also through Hh blockade.

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