5-fluorouracil drug alters the microrna expression profiles in MCF-7 breast cancer cells

2011 ◽  
Vol 226 (7) ◽  
pp. 1868-1878 ◽  
Author(s):  
Maitri Y. Shah ◽  
Xiaoping Pan ◽  
Lindsey N. Fix ◽  
Mary A. Farwell ◽  
Baohong Zhang
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Expression Profiles ◽  
Microrna Expression ◽  
Mcf 7

scholarly journals Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

2011 ◽  
Vol 313 (1) ◽  
pp. 26-43 ◽  
Author(s):  
Tissa T. Manavalan ◽  
Yun Teng ◽  
Savitri N. Appana ◽  
Susmita Datta ◽  
Theodore S. Kalbfleisch ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Differential Expression ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Microrna Expression ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Tamoxifen Resistant ◽  
Mcf 7

scholarly journals Cold Atmospheric Plasma Restores Paclitaxel Sensitivity to Paclitaxel-Resistant Breast Cancer Cells by Reversing Expression of Resistance-Related Genes

Cancers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2011 ◽  
Author(s):  
Sungbin Park ◽  
Heejoo Kim ◽  
Hwee Won Ji ◽  
Hyeon Woo Kim ◽  
Sung Hwan Yun ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Multiple Drug Resistance ◽  
Expression Profiles ◽  
Atmospheric Plasma ◽  
Multiple Drug ◽  
Cold Atmospheric Plasma ◽  
Mcf 7

Paclitaxel (Tx) is a widely used therapeutic chemical for breast cancer treatment; however, cancer recurrence remains an obstacle for improved prognosis of cancer patients. In this study, cold atmospheric plasma (CAP) was tested for its potential to overcome the drug resistance. After developing Tx-resistant MCF-7 (MCF-7/TxR) breast cancer cells, CAP was applied to the cells, and its effect on the recovery of drug sensitivity was assessed in both cellular and molecular aspects. Sensitivity to Tx in the MCF-7/TxR cells was restored up to 73% by CAP. A comparison of genome-wide expression profiles between the TxR cells and the CAP-treated cells identified 49 genes that commonly appeared with significant changes. Notably, 20 genes, such as KIF13B, GOLM1, and TLE4, showed opposite expression profiles. The protein expression levels of selected genes, DAGLA and CEACAM1, were recovered to those of their parental cells by CAP. Taken together, CAP inhibited the growth of MCF-7/TxR cancer cells and recovered Tx sensitivity by resetting the expression of multiple drug resistance–related genes. These findings may contribute to extending the application of CAP to the treatment of TxR cancer.

scholarly journals Effect of SMYD3 on the microRNA expression profile of MCF-7 breast cancer cells

2017 ◽  
Vol 14 (2) ◽  
pp. 1831-1840 ◽  
Author(s):  
Dongju Chen ◽  
Lei Liu ◽  
Xuegang Luo ◽  
Ai Mu ◽  
Lihua Yan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Expression Profile ◽  
Breast Cancer Cells ◽  
Microrna Expression ◽  
Microrna Expression Profile ◽  
Mcf 7

scholarly journals EFFECT OF 5-AZACYTIDINE ON miRNA EXPRESSION IN HUMAN BREAST CANCER CELLS WITH DIFFERENT SENSITIVITY TO CYTOSTATICS

2016 ◽  
Vol 38 (1) ◽  
pp. 26-30 ◽  
Author(s):  
V F Chehkun ◽  
T Borikun ◽  
N Yu Lukianova
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Mirna Expression ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Expression Profiles ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

Aim: To analyze expression of miRNA in human breast cancer cells, sensitive and resistant to cisplatin and doxorubicin, and to explore possible modification of drug sensitivity via treatment of cells with 5-azacytidine (5-aza), a demethylating agent. Materials and Methods: The study was performed on wild-type MCF-7 cell line (MCF-7/S) and its two sublines MCF-7/Dox and MCF-7/DDP resistant to doxorubicin and cisplatin, respectively. Cells were treated with 5-aza, cisplatin, doxorubicin and their combinations. Relative expression levels of miRNA-221, -200b, -320a, -10b, -34a, -122 and -29b were examined, using qRT-PCR. The MTT assay was used to monitor cell viability. Results: We compared miRNA expression profiles in MCF-7/S and drug resistant MCF-7/Dox and MCF-7/DDP cells. Changes of miRNA-221, -200b, -320a, -10b, -34a, -122 and -29b were observed in both resistant cell lines. The most significant differences were found for miRNA-200b (decreased in 50.0 ± 2.6 and 63.0 ± 3.1 times for MCF-7/Dox and MCF7/DDP cells, respectively) and for oncogenic miRNA-221 levels (increase in 62.0 ± 5.7 times for MCF-7/Dox and 83.8 ± 7.2 times for MCF-7/DDP cells). 5-aza treatment caused an increase of miRNA-10b, -122, -200b levels in MCF-7/S cells, miRNA-34a, -10b, -122, -200b and -320a levels in MCF-7/Dox cells and miRNA-34a, -10b, -200b and -320a levels in MCF-7/DDP cells. Pretreatment of all studied lines with 5-aza resulted in the increase of their sensitivity to studied cytostatics. In particular, the IC50 of doxorubicin decreased by 2-, 4- and 3-fold for cell lines MCF-7/S, MCF-7/Dox and MCF-7/DDP cells, respectively, and IC50 of cisplatin in studied cultures decreased by 3-, 2- and 1.5-fold, respectively. Conclusions: It was shown that use of 5-aza can modify sensitivity of breast cancer cells to cytotoxic drugs not only by it’s demetylation effect, but also by changes in expression of miRNAs, involved in cell proliferation, migration and drug resistance development.

scholarly journals MicroRNA expression profiles of drug-resistance breast cancer cells and their exosomes

Oncotarget ◽  
2016 ◽  
Vol 7 (15) ◽  
pp. 19601-19609 ◽  
Author(s):  
Shanliang Zhong ◽  
Xiu Chen ◽  
Dandan Wang ◽  
Xiaohui Zhang ◽  
Hongyu Shen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Expression Profiles ◽  
Microrna Expression
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