Constitutive overexpression of Id-1 in mammary glands of transgenic mice results in precocious and increased formation of terminal end buds, enhanced alveologenesis, delayed involution

2011 ◽  
Vol 226 (5) ◽  
pp. 1340-1352 ◽  
Author(s):  
Dong-Hui Shin ◽  
Si-Hyong Jang ◽  
Byeong-Cheol Kang ◽  
Hyun-Jun Kim ◽  
Seung Hyun Oh ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Mammary Glands ◽  
Terminal End Buds ◽  
Constitutive Overexpression

Regeneration of mammary glands in vivo from isolated mammary ducts

Development ◽  
1986 ◽  
Vol 96 (1) ◽  
pp. 229-243
Author(s):  
E. Jane Ormerod ◽  
Philip S. Rudland
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Cell Types ◽  
Mammary Glands ◽  
Myoepithelial Cells ◽  
Basal Cells ◽  
Alveolar Cells ◽  
Terminal End Buds ◽  
Mammary Cell

Rat mammary ducts, free of buds, can alone regenerate complete mammary trees when transplanted into the interscapular fat pads of syngeneic host rats. All the main mammary cell types are identified within such outgrowths. Epithelial cells, which show the presence of milk fat globule membrane antigens and microvilli on their luminal surfaces, line the ducts. Basal cells surrounding the ducts show characteristic features of myoepithelial cells: immunoreactive actin and keratin within the cytoplasm, myofilaments, pinocytotic vesicles and hemidesmosomal attachments to the basement membrane. Cells within the end buds and lateral buds, however, show few if any cytoplasmic myofilaments and are relatively undifferentiated in appearance. Intermediate morphologies between these cells and myoepithelial cells are seen nearer the ducts. In this respect they exactly resemble the cap cells found in terminal end buds (TEBs) of normal mammary glands. Occasional epithelial cells within alveolar buds show the presence of immunoreactive casein, which is a product of secretory alveolar cells in the normal rat mammary gland. Dissected terminal end buds can regenerate similar ductal outgrowths. Thus, ductal tissue alone can generate all the major mammary cell types seen in the normal gland, including the cap cells.

Generation of lobuloalveolar development from isolated rat mammary ducts and end buds.

1991 ◽  
Vol 39 (9) ◽  
pp. 1257-1266 ◽  
Author(s):  
P S Rudland
Keyword(s):  
Polyclonal Antibodies ◽  
Mammary Glands ◽  
Female Rats ◽  
Immunocytochemical Staining ◽  
Alveolar Cells ◽  
Peanut Lectin ◽  
Terminal End Buds ◽  
Lobuloalveolar Development ◽  
Fat Pads ◽  
Isolated Rat

Implantation of excised bud-free ductal fragments (DUCTS), terminal end buds (TEBs), or alveolar buds (ABs) from virgin mammary glands of Wistar-Furth rats into interscapular fat pads of syngeneic female rats produces, after 16 weeks, complete ductal outgrowths including TEBs and ABs. Treatment of the recipient rats with perphenazine for 1 day or mating them after 12 weeks and then isolating the resultant outgrowths after 16 weeks produces significantly larger outgrowths than those from untreated hosts. The outgrowths consist of distended ducts and lobules or distended ducts and alveoli, respectively. Histochemical and immunocytochemical staining of the outgrowths with reagents that depict epithelial, myoepithelial, and lactating alveolar cells (peanut lectin alone, monoclonal and polyclonal antibodies to rat caseins) indicate similar cell compositions and arrangements for all outgrowths irrespective of their source; these are also similar to the mammary glands of the perphenazine-stimulated or lactating hosts. There is one major difference: the degree of staining of peanut lectin alone and the anti-caseins is greater for outgrowths produced by the ABs and TEBs than for those produced by the DUCTs. DUCT implants left for 1 year after cessation of lactation of the hosts are still stained appreciably by peanut lectin alone and by the anti-caseins, particularly the luminal secretions. Therefore, the complete morphogenetic and cell differentiating ability for generating mammary glands is present in bud-free ducts, but this ability can be enhanced in TEBs/ABs or abnormally expressed at ectopic sites.

Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice

Biochimie ◽  
2011 ◽  
Vol 93 (3) ◽  
pp. 628-630 ◽  
Author(s):  
Veronika Yuskevich ◽  
Yuriy Khodarovich ◽  
German Kagarliskiy ◽  
Oleg Stremovskiy ◽  
Oksana Maksimenko ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Mammary Glands ◽  
Single Chain

Expression of Ovine Insulin-Like Growth Factor-1 (IGF-1) Stimulates Alveolar Bud Development in Mammary Glands of Transgenic Mice

Endocrine ◽  
1998 ◽  
Vol 8 (3) ◽  
pp. 251-260 ◽  
Author(s):  
Miriam S. Weber ◽  
Patricia L. Boyle ◽  
Benjamin A. Corl ◽  
Eric A. Wong ◽  
Frank C. Gwazdauskas ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Mammary Glands ◽  
Insulin Like Growth Factor ◽  
Bud Development

scholarly journals Overexpression of Des(1–3) Insulin-Like Growth Factor 1 in the Mammary Glands of Transgenic Mice Delays the Loss of Milk Production with Prolonged Lactation1

2005 ◽  
Vol 73 (6) ◽  
pp. 1116-1125 ◽  
Author(s):  
Darryl L. Hadsell ◽  
Daniel T. Torres ◽  
Nicole A. Lawrence ◽  
Jessy George ◽  
Albert F. Parlow ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Milk Production ◽  
Mammary Glands ◽  
Insulin Like Growth Factor

scholarly journals Diverse myeloid cells are recruited to the developing and inflamed mammary gland

2020 ◽  
Author(s):  
Gillian J Wilson ◽  
Ayumi Fukuoka ◽  
Francesca Vidler ◽  
Gerard J Graham
Keyword(s):  
Mammary Gland ◽  
Pubertal Timing ◽  
Myeloid Cell ◽  
Mammary Glands ◽  
Sterile Inflammation ◽  
Cell Compartment ◽  
Cell Recruitment ◽  
Terminal End Buds ◽  
Functional Consequences ◽  
Inflammatory Burden

AbstractThe immune system plays fundamental roles in the mammary gland, shaping developmental processes and controlling inflammation during infection and cancer. Here we reveal unanticipated heterogeneity in the myeloid cell compartment during development of virgin, pregnant and involuting mouse mammary glands, and in milk. We investigate the functional consequences of individual and compound chemokine receptor deficiency on cell recruitment. Diverse myeloid cell recruitment was also shown in models of sterile inflammation and bacterial infection. Strikingly, we have shown that inflammation and infection can alter the abundance of terminal end buds, a key developmental structure, within the pubertal mammary gland. This previously unknown effect of inflammatory burden during puberty could have important implications for understanding the control of pubertal timing.

scholarly journals Targeted c-myc gene expression in mammary glands of transgenic mice induces mammary tumours with constitutive milk protein gene transcription.

1988 ◽  
Vol 7 (1) ◽  
pp. 169-175 ◽  
Author(s):  
C. A. Schoenenberger ◽  
A. C. Andres ◽  
B. Groner ◽  
M. van der Valk ◽  
M. LeMeur ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Gene Transcription ◽  
Milk Protein ◽  
Protein Gene ◽  
Mammary Glands ◽  
Milk Protein Gene ◽  
Mammary Tumours ◽  
Myc Gene
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