Blood-derived human osteoclast resorption activity is impaired by Hyaluronan-CD44 engagement via a p38-dependent mechanism

2010 ◽  
Vol 226 (3) ◽  
pp. 769-779 ◽  
Author(s):  
Eliana Pivetta ◽  
Martina Scapolan ◽  
Bruna Wassermann ◽  
Agostino Steffan ◽  
Alfonso Colombatti ◽  
...  
Keyword(s):  
Human Osteoclast ◽  
Osteoclast Resorption ◽  
Dependent Mechanism

scholarly journals Surface Electric Fields Increase Human Osteoclast Resorption through Improved Wettability on Carbonate-Incorporated Apatite

2021 ◽  
Author(s):  
Leire Bergara-Muguruza ◽  
Keijo Mäkelä ◽  
Tommi Yrjälä ◽  
Jukka Salonen ◽  
Kimihiro Yamashita ◽  
...  
Keyword(s):  
Electric Fields ◽  
Human Osteoclast ◽  
Osteoclast Resorption

scholarly journals Surface electric fields increase human osteoclast resorption through improved wettability on carbonate-incorporated apatite

2021 ◽  
Author(s):  
Leire Bergara Muguruza ◽  
Keijo Makela ◽  
Tommi Yrjala ◽  
Jukka Salonen ◽  
Kimihiro Yamashita ◽  
...  
Keyword(s):  
Free Energy ◽  
Electric Fields ◽  
Bone Matrix ◽  
Surface Characteristics ◽  
Natural Mineral ◽  
Electrical Polarization ◽  
Mineral Phases ◽  
Human Osteoclast ◽  
Human Osteoclasts ◽  
Osteoclast Resorption

Osteoclast-mediated bioresorption can be of an efficient means of incorporating the dissolution of biomaterials in the bone remodeling process. Because of compositionally and structurally close resemblance of biomaterials with the natural mineral phases of the bone matrix, synthetic carbonate-substituted hydroxyapatite (CA) is considered as an ideal clinical biomaterial. The present study therefore investigated the effects of electrical polarization on the surface characteristics and interactions with human osteoclasts of hydroxyapatite (HA) and CA. Electrical polarization was found to improve the surface wettability of these materials by increasing the surface free energy, and this effect was maintained for one month. Analyses of human osteoclast cultures established that CA subjected to a polarization treatment accelerated osteoclast resorption but did not affect the early differentiation phase or the adherent morphology of the osteoclasts as evaluated by staining. These data suggest that the surface characteristics of the CA promoted osteoclast resorption. The results of this work are expected to contribute to the design of cell-mediated biomaterials that can be resorbed by osteoclasts after fulfilling their primary function as a scaffold for bone regeneration.

scholarly journals Potent and selective cathepsin L inhibitors do not inhibit human osteoclast resorption in vitro.

2003 ◽  
Vol 278 (34) ◽  
pp. 32484
Author(s):  
Ian E. James ◽  
Robert W. Marquis ◽  
Simon M. Blake ◽  
Shing Mei Hwang ◽  
Catherine J. Gress ◽  
...  
Keyword(s):  
Cathepsin L ◽  
Human Osteoclast ◽  
Osteoclast Resorption

Yeast RNA extract suppresses human osteoclast resorption in vitro

2017 ◽  
Vol 25 (5) ◽  
pp. 571-576
Author(s):  
Melissa D. Cantley ◽  
K. D. Rainsford ◽  
David R. Haynes
Keyword(s):  
Human Osteoclast ◽  
Osteoclast Resorption ◽  
Rna Extract

Platelet Mobilization Induced by PAF and Its Role in the Thrombocytosis Triggered by Adrenaline in Rats

1989 ◽  
Vol 62 (04) ◽  
pp. 1107-1111 ◽  
Author(s):  
Hugo C Castro-Faria-Neto ◽  
Patricia T Bozza ◽  
Marco A Martins ◽  
Paulo M F L Dias ◽  
Patricia M R Silva ◽  
...  
Keyword(s):  
Receptor Antagonists ◽  
Blood Samples ◽  
Platelet Counts ◽  
Platelet Number ◽  
Edta Solution ◽  
Web 2086 ◽  
Dependent Mechanism

SummaryThe injection of PAP (6 μg/kg, i. v.) induced, in rats, haemoconcentration accompanied by an increase in the platelet number, as attested by the counts of platelets in blood samples diluted in formalin-free EDTA solution. This increase was significant at 15 min, peaked from 1 to 4 h and returned to basal levels 24 h after the lipid administration. The release of platelets induced by PAP was inhibited dose-dependently by specific PAP receptor antagonists such as WEB 2086 (0.5-2 mg/kg), BN 52021 and 48740 RP (5-25 mg/kg). Furthermore, platelet mobilization was clearly impaired in splenectomized animals stimulated by PAP, whereas thrombocytopenia and haemoconcentration by the same stimulus were intact. It was also noted that a second injection of PAP, 24 h after the initial stimulation with the lipid, failed to induce an increase in platelet counts, indicating autodesensitization. Desensitization to PAP or pretreatment with PAP antagonists clearly prevented the increase in the platelet counts after stimulation by adrenaline (15 μg/kg). These findings suggest that, in rats, PAP can induce release of platelets by a spleen-dependent mechanism and that this lipid may be relevant to the thrombocytosis triggered by adrenaline.

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