Glutaminolysis and glycolysis regulation by troglitazone in breast cancer cells: Relationship to mitochondrial membrane potential

2010 ◽  
Vol 226 (2) ◽  
pp. 511-519 ◽  
Author(s):  
Ellen Friday ◽  
Robert Oliver ◽  
Tomas Welbourne ◽  
Francesco Turturro
Keyword(s):  
Breast Cancer ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Breast Cancer Cells ◽  
Glycolysis Regulation

Trabectedin to induce mitochondrial membrane potential dissipation and reactive oxygen species generation in breast cancer cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e13580-e13580
Author(s):  
Harika Atmaca ◽  
Emir Bozkurt ◽  
Burcu Cakar ◽  
Zeki Gokhan Surmeli ◽  
Selim Uzunoglu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Reactive Oxygen Species ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Dna Fragmentation ◽  
Human Breast Cancer ◽  
Mitochondrial Membrane ◽  
Breast Cancer Cells ◽  
Oxygen Species ◽  
Human Breast

e13580 Background: Trabectedin (Yondelis; ET-743) is a tetrahydroisoquinoline alkaloid that was originally derived from the marine tunicate Ecteinascidia turbinata and is currently prepared synthetically. It ıs currently used for the treatment of soft tissue sarcomas. It has been shown that Trabectedin provides the production of superoxides near the DNA strand, resulting in DNA backbone cleavage and cell apoptosis, however the apoptotic mechanisms of Trabectedin is still very limited. The objective of this study is to investigate the underlying mechanisms of apoptosis Trabectedin in two human breast cancer cell lines (MDA-MB-231 and MDA-MB-453) and one human immortalized non-transformed breast epithelial cell line (MCF-10A), to see if similar oxidation processes take place in breast cancer. Methods: Cytotoxicity was assessed by XTT cell viability assay. Apoptosis was shown by measuring DNA fragmentation, caspase 3/7 activation. Mitochondrial membrane potential (MMP) was evaluated by TMRE dye. Measurement of reactive oxygen species (ROS) was done by using Glutathione S-Transferase (GST) Assay Kit and CM-H2DCFDA dye. Results: Trabectedin induced the cytotoxicity in breast cancer cells in a time and dose dependent manner. Moreover, it increased DNA fragmentation and the MMP dissipation in tested breast cancer cells. The levels of ROS production in parallel with GST enzyme activity were sharply increased by Trabectedin treatment. Conclusions: This is the first study to investigate the role of Trabectedin activity and mechanisms of apoptosis in human breast cancer cells. These preliminary results might show us a way to use Trabectedin alone, or in combination for breast cancer treatment in near future.

Reserpine Induces Apoptosis and Cell Cycle Arrest in Hormone Independent Prostate Cancer Cells through Mitochondrial Membrane Potential Failure

2019 ◽  
Vol 18 (9) ◽  
pp. 1313-1322 ◽  
Author(s):  
Manjula Devi Ramamoorthy ◽  
Ashok Kumar ◽  
Mahesh Ayyavu ◽  
Kannan Narayanan Dhiraviam
Keyword(s):  
Prostate Cancer ◽  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Prostate Cancer Cells

Background: Reserpine, an indole alkaloid commonly used for hypertension, is found in the roots of Rauwolfia serpentina. Although the root extract has been used for the treatment of cancer, the molecular mechanism of its anti-cancer activity on hormonal independent prostate cancer remains elusive. Methods: we evaluated the cytotoxicity of reserpine and other indole alkaloids, yohimbine and ajmaline on Prostate Cancer cells (PC3) using MTT assay. We investigated the mechanism of apoptosis using a combination of techniques including acridine orange/ethidium bromide staining, high content imaging of Annexin V-FITC staining, flow cytometric quantification of the mitochondrial membrane potential and Reactive Oxygen Species (ROS) and cell cycle analysis. Results: Our results indicate that reserpine inhibits DNA synthesis by arresting the cells at the G2 phase and showed all standard sequential features of apoptosis including, destabilization of mitochondrial membrane potential, reduced production of reactive oxygen species and DNA ladder formation. Our in silico analysis further confirmed that indeed reserpine docks to the catalytic cleft of anti-apoptotic proteins substantiating our results. Conclusion: Collectively, our findings suggest that reserpine can be a novel therapeutic agent for the treatment of androgen-independent prostate cancer.

The repopulating cancer cells in melanoma are characterized by increased mitochondrial membrane potential

2016 ◽  
Vol 382 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Don G. Lee ◽  
Beom K. Choi ◽  
Young H. Kim ◽  
Ho S. Oh ◽  
Sang H. Park ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane

scholarly journals Quantitative analysis of mitochondrial membrane potential heterogeneity in unsynchronized and synchronized cancer cells

2020 ◽  
Vol 35 (1) ◽  
Author(s):  
Amandine Rovini ◽  
Kareem Heslop ◽  
Elizabeth G. Hunt ◽  
Morgan E. Morris ◽  
Diana Fang ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Quantitative Analysis ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane

Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells

2009 ◽  
Vol 54 (23) ◽  
pp. 4387-4392 ◽  
Author(s):  
Yeon-Suk Oh ◽  
Hee-Young Kwon ◽  
Soo-Jin Jeong ◽  
Ki-Young Park ◽  
Sun-Young Kim ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Caspase 3

scholarly journals Emodin, isolated and characterized from an endophytic fungus Polyporales sp., induces apoptotic cell death in human lung cancer cells through the loss of mitochondrial membrane potential

2017 ◽  
Vol 6 (5) ◽  
pp. 288-292
Author(s):  
Refaz Ahmad Dar ◽  
◽  
Rabiya Majeed ◽  
Abid Ali sheikh ◽  
Shakeel-u Rehman ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Endophytic Fungus ◽  
Mitochondrial Membrane ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Close Relative ◽  
Dependent Manner

Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a Chinese herbal anthraquinone that exhibits numerous biological activities, such as antitumor, antibacterial, antiinflammatory, and immunosuppressive. From an endophytic fungus, a close relative of Polyporales sp., found in association with Rheum emodi, Wall. ex Meissn a compound (Rz) was isolated and characterizedby different spectroscopic techniques (1H-NMR, 13CNMR, 2D-NMR, and HRMS). The compound (Rz) displayed a range of cytotoxicities against different human cancer cell lines like THP-1(Leukemia), A549 (Lung), NCI-H322 (lung) and Colo-205(colon) at a concentration of 70 and 100 µM. The compound had strong anticancer activity by arresting the cell cycle at G1 and G2/M phase and loss of mitochondrial membrane potential in A-549 lung cancer cells in concentration dependent manner. The study suggests that emodin induced anticancer effects may have novel therapeutic applications for the treatment of lung cancer.

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