Neurite outgrowth from PC12 cells by basic fibroblast growth factor (bFGF) is mediated by RhoA inactivation through p190RhoGAP and ARAP3

2010 ◽  
Vol 224 (3) ◽  
pp. 786-794 ◽  
Author(s):  
Chan-Young Jeon ◽  
Hee-Jun Kim ◽  
Hiroshi Morii ◽  
Nozomu Mori ◽  
Jeffrey Settleman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Neurite Outgrowth ◽  
Fibroblast Growth Factor ◽  
Pc12 Cells ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth

scholarly journals Basic fibroblast growth factor is an extracellular matrix component required for supporting the proliferation of vascular endothelial cells and the differentiation of PC12 cells.

1989 ◽  
Vol 109 (2) ◽  
pp. 823-831 ◽  
Author(s):  
S Rogelj ◽  
M Klagsbrun ◽  
R Atzmon ◽  
M Kurokawa ◽  
A Haimovitz ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Fibroblast Growth Factor ◽  
Pc12 Cells ◽  
Basic Fibroblast Growth Factor ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Fibroblast Growth

Vascular endothelial cells (ECs) seeded sparsely on extracellular matrix (ECM) will proliferate in the absence of exogenous basic fibroblast growth factor (bFGF). This ECM will also stimulate neurite outgrowth in PC12 cells in the absence of exogenous growth factors. We have previously shown that bFGF is found in subendothelial ECM (Vlodavsky, I., J. Folkman, R. Sullivan, R. Fridman, R. Ishai-Michaeli, J. Sasse, and M. Klagsburn. 1987. Proc. Natl. Acad. Sci. USA. 84:2292-2296) and in basement membranes (Folkman, J., M. Klagsburn, J. Sasse, M. Wadzinski, D. Ingber, and I. Vlodavsky. 1988. Am. J. Pathol. 130:393-400). The actual requirement of ECM-associated bFGF for the growth of ECs and differentiation of PC12 cells was shown in two ways. First, polyclonal anti-bFGF antibodies added to subendothelial ECM inhibited both EC proliferation and PC12 neurite outgrowth. Secondly, PF-HR-9 cells, which do not synthesize bFGF and which produce an ECM not permissive for EC proliferation and PC12 neurite outgrowth, were transfected with bFGF cDNA. PF-HR-9 cells transfected with bFGF, but not with the dominant selectable marker SV2-neomycin, were found to express bFGF and to produce an ECM which did support both EC proliferation and PC12 differentiation. The ECM-mediated stimulatory effects were inhibited by anti-bFGF antibodies but not by anti-nerve growth factor antibodies or nonimmune rabbit IgG. These results indicate that bFGF associated with ECM is a required ECM component for ECM-mediated cell proliferation and differentiation.

scholarly journals Erratum: Neurite outgrowth from PC12 cells by basic fibroblast growth factor (bFGF) is mediated by RhoA inactivation through p190RhoGAP and ARAP3

2011 ◽  
Vol 226 (4) ◽  
pp. 1137-1137
Author(s):  
Chan-Young Jeon ◽  
Hee-Jun Kim ◽  
Hiroshi Morii ◽  
Nozomu Mori ◽  
Jeffrey Settleman ◽  
...  
Keyword(s):  
Growth Factor ◽  
Neurite Outgrowth ◽  
Fibroblast Growth Factor ◽  
Pc12 Cells ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth

Characterization of an endooligopeptidase A-like protein in PC12 cells: Activity modulation by cAMP but not by basic fibroblast growth factor

1995 ◽  
Vol 57 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Emer S. Ferro ◽  
Denise V. Tambourgy ◽  
Patricia A. E. Abreu ◽  
Antonio C. M. Camargo ◽  
Isaias Raw ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Pc12 Cells ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth
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