Characterization of BAG3 cleavage during apoptosis of pancreatic cancer cells

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Hua-Qin Wang ◽  
Xin Meng ◽  
Yan-Yan Gao ◽  
Bao-Qin Liu ◽  
Xiao-Fang Niu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Pancreatic Cancer Cells

Galectin-3 is modulated in pancreatic cancer cells under hypoxia and nutrient deprivation

2020 ◽  
Vol 401 (10) ◽  
pp. 1153-1165 ◽  
Author(s):  
Antônio F. da Silva Filho ◽  
Lucas B. Tavares ◽  
Maira G. R. Pitta ◽  
Eduardo I. C. Beltrão ◽  
Moacyr J. B. M. Rêgo
Keyword(s):  
Pancreatic Cancer ◽  
Cell Death ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Ductal Adenocarcinoma ◽  
Reverse Transcription Pcr ◽  
Pancreatic Cancer Cells ◽  
Through Flow ◽  
Galectin 3

AbstractPancreatic ductal adenocarcinoma is one of the most aggressive tumors with a microenvironment marked by hypoxia and starvation. Galectin-3 has been evaluated in solid tumors and seems to present both pro/anti-tumor effects. So, this study aims to characterize the expression of Galectin-3 from pancreatic tumor cells and analyze its influence for cell survive and motility in mimetic microenvironment. For this, cell cycle and cell death were accessed through flow cytometry. Characterization of inside and outside Galectin-3 was performed through Real-Time Quantitative Reverse Transcription PCR (qRT-PCR), immunofluorescence, Western blot, and ELISA. Consequences of Galectin-3 extracellular inhibition were investigated using cell death and scratch assays. PANC-1 showed increased Galectin-3 mRNA expression when cultivated in hypoxia for 24 and 48 h. After 24 h in simultaneously hypoxic/deprived incubation, PANC-1 shows increased Galectin-3 protein and secreted levels. For Mia PaCa-2, cultivation in deprivation was determinant for the increasing in Galectin-3 mRNA expression. When cultivated in simultaneously hypoxic/deprived condition, Mia PaCa-2 also presented increasing for the Galectin-3 secreted levels. Treatment of PANC-1 cells with lactose increased the death rate when cells were incubated simultaneously hypoxic/deprived condition. Therefore, it is possible to conclude that the microenvironmental conditions modulate the Galectin-3 expression on the transcriptional and translational levels for pancreatic cancer cells.

SPROUTY2 EXPRESSION IN HUMAN PANCREATIC CANCER CELLS AND CHARACTERIZATION OF THE SPROUTY2 PROMOTER.

Pancreas ◽  
2004 ◽  
Vol 29 (4) ◽  
pp. 335-336
Author(s):  
C.Y. Chan ◽  
U. Burley ◽  
X.Z. Ding ◽  
M.S. Talamonti ◽  
R.H. Bell ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells

Characterization of the Bystander Effect of Somatostatin Receptor sst2 After In Vivo Gene Transfer into Human Pancreatic Cancer Cells

2005 ◽  
Vol 16 (10) ◽  
pp. 1175-1193 ◽  
Author(s):  
Nicolas Carrere ◽  
Fabienne Vernejoul ◽  
Anny Souque ◽  
Amani Asnacios ◽  
Nicole Vaysse ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Gene Transfer ◽  
Cancer Cells ◽  
Bystander Effect ◽  
Somatostatin Receptor ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells ◽  
In Vivo Gene Transfer

Abstract 1902: Characterization of choline phospholipid metabolism in pancreatic cancer cells.

2013 ◽  
Author(s):  
Tariq Shah ◽  
Flonne Wildes ◽  
Yelena Mironchik ◽  
Marie-France Penet ◽  
Anirban Maitra ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Phospholipid Metabolism ◽  
Pancreatic Cancer Cells ◽  
Choline Phospholipid

scholarly journals Characterization of proteins secreted by pancreatic cancer cells with anticancer drug treatment in vitro

2012 ◽  
Vol 28 (6) ◽  
pp. 1968-1976 ◽  
Author(s):  
TAKANOBU TAKATA ◽  
YASUHITO ISHIGAKI ◽  
TAKEO SHIMASAKI ◽  
HIDEYUKI TSUCHIDA ◽  
YOSHIHARU MOTOO ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Drug Treatment ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Pancreatic Cancer Cells

Abstract A23: Characterization of novel molecular vulnerabilities provoking replicative and energetic stresses in pancreatic cancer cells

2016 ◽  
Author(s):  
Marion Gayral ◽  
Audrey Frances ◽  
Estelle Saland ◽  
Hubert Lulka ◽  
Naima Hanoun ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Pancreatic Cancer Cells
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