Self-renewal and pluripotency is maintained in human embryonic stem cells by co-culture with human fetal liver stromal cells expressing hypoxia inducible factor 1α

2009 ◽  
Vol 221 (1) ◽  
pp. 54-66 ◽  
Author(s):  
Lei Ji ◽  
Yu-xiao Liu ◽  
Chao Yang ◽  
Wen Yue ◽  
Shuang-shuang Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Hypoxia Inducible Factor ◽  
Embryonic Stem ◽  
Self Renewal ◽  
Human Fetal Liver ◽  
Hypoxia Inducible Factor 1Α

scholarly journals Human Fetal Liver Stromal Cells That Overexpress bFGF Support Growth and Maintenance of Human Embryonic Stem Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e14457 ◽  
Author(s):  
Jiafei Xi ◽  
Yunfang Wang ◽  
Peng Zhang ◽  
Lijuan He ◽  
Xue Nan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Embryonic Stem ◽  
Human Fetal Liver

A Novel Method for Efficient Production of Multipotential Hematopoietic Progenitors from Human Embryonic Stem Cells by Co-Culture with Murine Fetal Liver-Derived Stromal Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4214-4214
Author(s):  
Feng Ma ◽  
Dan Wang ◽  
Sachiyo Hanada ◽  
Hirohide Kawasaki ◽  
Yuji Zaike ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Stromal Cells ◽  
Blood Cells ◽  
Fetal Liver ◽  
Hematopoietic Cells ◽  
Embryonic Stem ◽  
Efficient Production ◽  
Hematopoietic Progenitors

Abstract Human embryonic stem cells provide a unique tool to study early events occurring in the development of human embryonic hematopoiesis, and their totipotent capability indicates a potent clinical application based on the cellular therapy and the evaluation of drug effects on hematopoietic and blood cells. To achieve efficient production of hematopoietic cells from human embryonic stem cells, we attempted to reproduce the circumstance surrounding embryonic hematopoietic cells in vitro. Since fetal liver is the predominant source of hematopoietic and blood cells in mammalian embryogenesis, we established stromal cells from mouse fetal liver at days 14 to 15 of gestation. In the co-culture of human embryonic stem cells with the established stromal cells, a number of hematopoietic progenitors were generated at around day 14 of co-culture, and this hematopoietic activity was highly enriched in the cobble stone-like cells under the stromal layer. Most of the cobble stone-like cells collected expressed CD34 and contained a variety of hematopoietic colony-forming cells, especially multilineage colony-forming cells, at a high frequency. The multipotential hematopoietic progenitors in the cobble stone-like cells produced all types of mature blood cells, including adult type hemoglobin-synthesizing erythrocytes and tryptase and chymase-bouble positive mast cells in the suspension cultiue with a cytokine cocktail. The developed co-culture system of human embryonic stem cells should offer a novel source for hematopoietic and blood cells applicable to cellular therapies and drug screening.

scholarly journals Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver

2009 ◽  
Vol 19 (6) ◽  
pp. 1044-1056 ◽  
Author(s):  
A. L. Brunner ◽  
D. S. Johnson ◽  
S. W. Kim ◽  
A. Valouev ◽  
T. E. Reddy ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Fetal Liver ◽  
Embryonic Stem ◽  
Human Fetal Liver ◽  
Methylation Patterns

scholarly journals Immortalized mouse fetal liver stromal cells support growth and maintenance of human embryonic stem cells

2012 ◽  
Vol 28 (4) ◽  
pp. 1385-1391 ◽  
Author(s):  
JIABO HU ◽  
SANQIANG HU ◽  
QUANHUI MA ◽  
XIAOHUI WANG ◽  
ZHONGWEI ZHOU ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Embryonic Stem

scholarly journals Effects of Ionizing Radiation on Self-Renewal and Pluripotency of Human Embryonic Stem Cells

2010 ◽  
Vol 70 (13) ◽  
pp. 5539-5548 ◽  
Author(s):  
Kitchener D. Wilson ◽  
Ning Sun ◽  
Mei Huang ◽  
Wendy Y. Zhang ◽  
Andrew S. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Ionizing Radiation ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals Environmental Oxygen Tension Regulates the Energy Metabolism and Self-Renewal of Human Embryonic Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62507 ◽  
Author(s):  
Catherine E. Forristal ◽  
David R. Christensen ◽  
Fay E. Chinnery ◽  
Raffaella Petruzzelli ◽  
Kate L. Parry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Energy Metabolism ◽  
Oxygen Tension ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

Monocytic cells derived from human embryonic stem cells and fetal liver share common differentiation pathways and homeostatic functions

Blood ◽  
2011 ◽  
Vol 117 (11) ◽  
pp. 3065-3075 ◽  
Author(s):  
Olena Klimchenko ◽  
Antonio Di Stefano ◽  
Birgit Geoerger ◽  
Sofiane Hamidi ◽  
Paule Opolon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Fetal Liver ◽  
Critical Role ◽  
Embryonic Stem ◽  
Blood Monocytes ◽  
Monocytic Cell ◽  
Monocytic Cells

Abstract The early emergence of macrophages and their large pattern of tissue distribution during development suggest that they may play a critical role in the initial steps of embryogenesis. In the present study, we show that monocytic cells derived from human embryonic stem cells (hESCs) and from fetal liver follow a differentiation pathway different to that of adult cells, leading to specific functions. Embryonic and fetal monocytic cells differentiated from a CD14lowCD16− precursor to form CD14highCD16+ cells without producing the CD14highCD16− cell population that predominates in adult peripheral blood. Both demonstrated an enhanced expression of genes encoding tissue-degrading enzymes, chemokines, and scavenger receptors, as was previously reported for M2 macrophages. Compared with adult blood monocytes, embryonic and fetal monocytic cells secreted high amounts of proteins acting on tissue remodeling and angiogenesis, and most of them expressed the Tie2 receptor. Furthermore, they promoted vascular remodeling in xenotransplanted human tumors. These findings suggest that the regulation of human fetal and embryonic monocytic cell differentiation leads to the generation of cells endowed mainly with anti-inflammatory and remodeling functions. Trophic and immunosuppressive functions of M2-polarized macrophages link fetus and tumor development, and hESCs offer a valuable experimental model for in vitro studies of mechanisms sustaining these processes.

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