5′-N-ethylcarboxamide induces IL-6 expression via MAPKs and NF-κB activation through Akt, Ca2+/PKC, cAMP signaling pathways in mouse embryonic stem cells

2009 ◽  
Vol 219 (3) ◽  
pp. 752-759 ◽  
Author(s):  
Mi Ok Kim ◽  
Min Hee Kim ◽  
Sang Hun Lee ◽  
Han Na Suh ◽  
Yu Jin Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Camp Signaling

Functional expression of Ca2+ signaling pathways in mouse embryonic stem cells

Cell Calcium ◽  
2004 ◽  
Vol 36 (2) ◽  
pp. 135-146 ◽  
Author(s):  
Eri Yanagida ◽  
Satoshi Shoji ◽  
Yoshiyuki Hirayama ◽  
Fumio Yoshikawa ◽  
Keishi Otsu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Functional Expression ◽  
Mouse Embryonic Stem Cells

Signaling pathways regulating proliferation of mouse embryonic stem cells

2007 ◽  
Vol 1 (3) ◽  
pp. 191-205 ◽  
Author(s):  
I. A. Chuykin ◽  
M. S. Lianguzova ◽  
V. A. Pospelov
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals ChIP-Seq and RNA-Seq Analyses Identify Components of the Wnt and Fgf Signaling Pathways as Prep1 Target Genes in Mouse Embryonic Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0122518 ◽  
Author(s):  
Audrey Laurent ◽  
Manuela Calabrese ◽  
Hans-Jörg Warnatz ◽  
Marie-Laure Yaspo ◽  
Vsevolod Tkachuk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Target Genes ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Rna Seq ◽  
Fgf Signaling

scholarly journals Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Jan Kučera ◽  
Lucia Binó ◽  
Kateřina Štefková ◽  
Josef Jaroš ◽  
Ondřej Vašíček ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Intracellular Signaling ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cellular Functions ◽  
Differentiation Status ◽  
Canonical Wnt ◽  
Erk Activity

Reactive oxygen species (ROS) are important regulators of cellular functions. In embryonic stem cells, ROS are suggested to influence differentiation status. Regulated ROS formation is catalyzed primarily by NADPH-dependent oxidases (NOXs). Apocynin and diphenyleneiodonium are frequently used inhibitors of NOXs; however, both exhibit uncharacterized effects not related to NOXs inhibition. Interestingly, in our model of mouse embryonic stem cells we demonstrate low expression of NOXs. Therefore we aimed to clarify potential side effects of these drugs. Both apocynin and diphenyleneiodonium impaired proliferation of cells. Surprisingly, we observed prooxidant activity of these drugs determined by hydroethidine. Further, we revealed that apocynin inhibits PI3K/Akt pathway with its downstream transcriptional factor Nanog. Opposite to this, apocynin augmented activity of canonical Wnt signaling. On the contrary, diphenyleneiodonium activated both PI3K/Akt and Erk signaling pathways without affecting Wnt. Our data indicates limits and possible unexpected interactions of NOXs inhibitors with intracellular signaling pathways.

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