scholarly journals CDK inhibitors selectively diminish cell cycle controlled activation of the histone H4 gene promoter by p220NPATand HiNF-P

2009 ◽  
Vol 219 (2) ◽  
pp. 438-448 ◽  
Author(s):  
Partha Mitra ◽  
Prachi N. Ghule ◽  
Margaretha van der Deen ◽  
Ricardo Medina ◽  
Rong-lin Xie ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Gene Promoter ◽  
Cdk Inhibitors ◽  
Histone H4 ◽  
Histone H4 Gene

Cell cycle regulation of a mouse histone H4 gene requires the H4 promoter

1987 ◽  
Vol 7 (3) ◽  
pp. 1048-1054
Author(s):  
A Seiler-Tuyns ◽  
B M Paterson
Keyword(s):  
Cell Cycle ◽  
Shuttle Vector ◽  
Constitutive Expression ◽  
Mrna Levels ◽  
Histone H4 ◽  
Coding Region ◽  
Regulated Expression ◽  
Arrest Growth ◽  
Globin Promoter ◽  
Histone H4 Gene

The mouse histone H4 gene, when stably transformed into L cells on the PSV2gpt shuttle vector, is cell cycle regulated in parallel with the endogenous H4 genes. This was determined in exponentially growing pools of transformants fractionated into cell cycle-specific stages by centrifugal elutriation, a method for purifying cells at each stage of the cell cycle without the use of treatments that arrest growth. Linker additions in the 5' noncoding region of the H4 RNA or in the coding region of the gene did not affect the cell cycle-regulated expression of the modified H4 gene even though the overall level of expression was altered. However, replacing the H4 promoter with the human alpha-2 globin promoter, so that the histone transcript produced by the chimeric gene remains essentially unchanged, resulted in the constitutive expression of H4 mRNA during all phases of the cell cycle with no net increase in H4 mRNA levels during the G1-to-S transition. From these results we conclude that all the information necessary for the cell cycle-regulated expression of the H4 gene is contained in the 5.2-kilobase subclone used in these studies with 228 nucleotides of 5'-flanking DNA and that the increase in H4 mRNA during the G1-to-S transition in the cell cycle is mediated by the H4 promoter and not by the increased stability of the H4 RNA.

ATF1 and CREB Trans-Activate a Cell Cycle Regulated Histone H4 Gene at a Distal Nuclear Matrix Associated Promoter Element†

Biochemistry ◽  
1997 ◽  
Vol 36 (47) ◽  
pp. 14447-14455 ◽  
Author(s):  
Bo Guo ◽  
Janet L. Stein ◽  
André J. van Wijnen ◽  
Gary S. Stein
Keyword(s):  
Cell Cycle ◽  
Nuclear Matrix ◽  
Promoter Element ◽  
Histone H4 ◽  
A Cell ◽  
Histone H4 Gene

scholarly journals Identification of HiNF-P, a Key Activator of Cell Cycle-Controlled Histone H4 Genes at the Onset of S Phase

2003 ◽  
Vol 23 (22) ◽  
pp. 8110-8123 ◽  
Author(s):  
Partha Mitra ◽  
Rong-Lin Xie ◽  
Ricardo Medina ◽  
Hayk Hovhannisyan ◽  
S. Kaleem Zaidi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cyclin E ◽  
S Phase ◽  
Phase Cell ◽  
Regulatory Sequences ◽  
Histone H4 ◽  
Restriction Point ◽  
Histone H4 Gene

ABSTRACT At the G1/S phase cell cycle transition, multiple histone genes are expressed to ensure that newly synthesized DNA is immediately packaged as chromatin. Here we have purified and functionally characterized the critical transcription factor HiNF-P, which is required for E2F-independent activation of the histone H4 multigene family. Using chromatin immunoprecipitation analysis and ligation-mediated PCR-assisted genomic sequencing, we show that HiNF-P interacts with conserved H4 cell cycle regulatory sequences in vivo. Antisense inhibition of HiNF-P reduces endogenous histone H4 gene expression. Furthermore, we find that HiNF-P utilizes NPAT/p220, a substrate of the cyclin E/cyclin-dependent kinase 2 (CDK2) kinase complex, as a key coactivator to enhance histone H4 gene transcription. The biological role of HiNF-P is reflected by impeded cell cycle progression into S phase upon antisense-mediated reduction of HiNF-P levels. Our results establish that HiNF-P is the ultimate link in a linear signaling pathway that is initiated with the growth factor-dependent induction of cyclin E/CDK2 kinase activity at the restriction point and culminates in the activation of histone H4 genes through HiNF-P at the G1/S phase transition.

scholarly journals HiNF-P Directly Links the Cyclin E/CDK2/p220NPAT Pathway to Histone H4 Gene Regulation at the G1/S Phase Cell Cycle Transition

2005 ◽  
Vol 25 (14) ◽  
pp. 6140-6153 ◽  
Author(s):  
Angela Miele ◽  
Corey D. Braastad ◽  
William F. Holmes ◽  
Partha Mitra ◽  
Ricardo Medina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Phase Transition ◽  
Cell Cycle ◽  
Cyclin E ◽  
S Phase ◽  
Phase Cell ◽  
Genome Replication ◽  
Dependent Manner ◽  
Histone H4 ◽  
Histone H4 Gene

ABSTRACT Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the mechanism that links the restriction (R) point late in G1 with histone gene expression at the onset of S phase. Here we demonstrate that HiNF-P, a transcriptional regulator of replication-dependent histone H4 genes, interacts directly with p220NPAT, a substrate of cyclin E/CDK2, to coactivate histone genes during S phase. HiNF-P and p220 are targeted to, and colocalize at, subnuclear foci (Cajal bodies) in a cell cycle-dependent manner. Genetic or biochemical disruption of the HiNF-P/p220 interaction compromises histone H4 gene activation at the G1/S phase transition and impedes cell cycle progression. Our results show that HiNF-P and p220 form a critical regulatory module that directly links histone H4 gene expression at the G1/S phase transition to the cyclin E/CDK2 signaling pathway at the R point.

scholarly journals The Cell Cycle Control Element of Histone H4 Gene Transcription Is Maximally Responsive to Interferon Regulatory Factor Pairs IRF-1/IRF-3 and IRF-1/IRF-7

2001 ◽  
Vol 276 (21) ◽  
pp. 18624-18632 ◽  
Author(s):  
Ronglin Xie ◽  
André J. van Wijnen ◽  
Caroline van der Meijden ◽  
Mai X. Luong ◽  
Janet L. Stein ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Gene Transcription ◽  
Cell Cycle Control ◽  
Interferon Regulatory Factor ◽  
Control Element ◽  
Histone H4 ◽  
Regulatory Factor ◽  
Histone H4 Gene

scholarly journals Cell cycle regulation of a mouse histone H4 gene requires the H4 promoter.

1987 ◽  
Vol 7 (3) ◽  
pp. 1048-1054 ◽  
Author(s):  
A Seiler-Tuyns ◽  
B M Paterson
Keyword(s):  
Cell Cycle ◽  
Shuttle Vector ◽  
Constitutive Expression ◽  
Mrna Levels ◽  
Histone H4 ◽  
Coding Region ◽  
Regulated Expression ◽  
Arrest Growth ◽  
Globin Promoter ◽  
Histone H4 Gene

The mouse histone H4 gene, when stably transformed into L cells on the PSV2gpt shuttle vector, is cell cycle regulated in parallel with the endogenous H4 genes. This was determined in exponentially growing pools of transformants fractionated into cell cycle-specific stages by centrifugal elutriation, a method for purifying cells at each stage of the cell cycle without the use of treatments that arrest growth. Linker additions in the 5' noncoding region of the H4 RNA or in the coding region of the gene did not affect the cell cycle-regulated expression of the modified H4 gene even though the overall level of expression was altered. However, replacing the H4 promoter with the human alpha-2 globin promoter, so that the histone transcript produced by the chimeric gene remains essentially unchanged, resulted in the constitutive expression of H4 mRNA during all phases of the cell cycle with no net increase in H4 mRNA levels during the G1-to-S transition. From these results we conclude that all the information necessary for the cell cycle-regulated expression of the H4 gene is contained in the 5.2-kilobase subclone used in these studies with 228 nucleotides of 5'-flanking DNA and that the increase in H4 mRNA during the G1-to-S transition in the cell cycle is mediated by the H4 promoter and not by the increased stability of the H4 RNA.

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