Hepatocyte growth factor modulates Sertoli–Sertoli tight junction dynamics

2008 ◽  
Vol 216 (1) ◽  
pp. 253-260 ◽  
Author(s):  
A. Catizone ◽  
G. Ricci ◽  
M. Galdieri
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Tight Junction ◽  
Hepatocyte Growth

Hepatocyte growth factor induces MDCK cell morphogenesis without causing loss of tight junction functional integrity

2004 ◽  
Vol 286 (3) ◽  
pp. C482-C494 ◽  
Author(s):  
Anne L. Pollack ◽  
Gerard Apodaca ◽  
Keith E. Mostov
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Tight Junction ◽  
Mdck Cell ◽  
Mdck Cells ◽  
Peak Effect ◽  
Ruthenium Red ◽  
Functional Integrity ◽  
Organ Regeneration ◽  
Hepatocyte Growth

Hepatocyte growth factor (HGF) induces mitogenesis, motogenesis, and tubulogenesis of cultured Madin-Darby canine kidney (MDCK) epithelial cells. We report that in addition to these effects HGF stimulates morphogenesis of tight, polarized MDCK cell monolayers into pseudostratified layers without loss of tight junction (TJ) functional integrity. We tested TJ functional integrity during formation of pseudostratified layers. In response to HGF, the TJ marker ZO-1 remained in morphologically complete rings and functional barriers to paracellular diffusion of ruthenium red were maintained in pseudostratified layers. Transepithelial resistance (TER) increased transiently two- to threefold during the morphogenetic transition from monolayers to pseudostratified layers and then declined to baseline levels once pseudostratified layers were formed. In MDCK cells expressing the trk/met chimera, both HGF and NGF at concentrations of 2.5 ng/ml induced scattering. However, 2.5 ng/ml HGF did not affect TER. The peak effect of HGF on TER was at a concentration of 100 ng/ml. In contrast, NGF at concentrations as high as 25 μg/ml had no effect on TER or pseudostratified layer morphogenesis of trk/met-expressing cultures. These results suggest that altered presentation of the stimulus, such as through HGF interaction with low-affinity sites, may change the downstream signaling response. In addition, our results demonstrate that HGF stimulates pseudostratified layer morphogenesis while inducing an increase in TER and maintaining the overall tightness of the epithelial layer. Stimulation of epithelial cell movements by HGF without loss of functional TJs may be important for maintaining epithelial integrity during morphogenetic events such as formation of pseudostratified epithelia, organ regeneration, and tissue repair.

scholarly journals Hepatocyte Growth Factor stimulated cell scattering requires ERK and Cdc42-dependent tight junction disassembly

2010 ◽  
Vol 400 (2) ◽  
pp. 271-277 ◽  
Author(s):  
Akashi Togawa ◽  
Jeffery Sfakianos ◽  
Shuta Ishibe ◽  
Sayuri Suzuki ◽  
Yoshihide Fujigaki ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Tight Junction ◽  
Cell Scattering ◽  
Hepatocyte Growth

Role of Bcl-xL in hepatocyte growth factor-elicited epithelial protection in idiopathic pulmonary fibrosis

Pneumologie ◽  
2014 ◽  
Vol 68 (06) ◽  
Author(s):  
S Skwarna ◽  
I Henneke ◽  
W Seeger ◽  
T Geiser ◽  
A Günther ◽  
...  
Keyword(s):  
Growth Factor ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Hepatocyte Growth Factor ◽  
Hepatocyte Growth

Potential role of an endothelium-specific growth factor, hepatocyte growth factor, on endothelial damage in diabetes

Diabetes ◽  
1997 ◽  
Vol 46 (1) ◽  
pp. 138-142 ◽  
Author(s):  
R. Morishita ◽  
S. Nakamura ◽  
Y. Nakamura ◽  
M. Aoki ◽  
A. Moriguchi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Potential Role ◽  
Specific Growth ◽  
Endothelial Damage ◽  
Specific Growth Factor ◽  
Hepatocyte Growth

Culture of adult human islet preparations with hepatocyte growth factor and 804G matrix is mitogenic for duct cells but not for beta-cells

Diabetes ◽  
1998 ◽  
Vol 47 (1) ◽  
pp. 134-137 ◽  
Author(s):  
V. H. Lefebvre ◽  
T. Otonkoski ◽  
J. Ustinov ◽  
M. A. Huotari ◽  
D. G. Pipeleers ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Beta Cells ◽  
Human Islet ◽  
Adult Human ◽  
Duct Cells ◽  
Hepatocyte Growth
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