scholarly journals Cancer testis antigens in human melanoma stem cells: Expression, distribution, and methylation status

2008 ◽  
Vol 215 (2) ◽  
pp. 287-291 ◽  
Author(s):  
Luca Sigalotti ◽  
Alessia Covre ◽  
Susan Zabierowski ◽  
Benjamin Himes ◽  
Francesca Colizzi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Methylation Status ◽  
Human Melanoma ◽  
Cancer Testis Antigens ◽  
Cancer Testis

scholarly journals Expression Patterns of Cancer-Testis Antigens in Human Embryonic Stem Cells and Their Cell Derivatives Indicate Lineage Tracks

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Nadya Lifantseva ◽  
Anna Koltsova ◽  
Tatyana Krylova ◽  
Tatyana Yakovleva ◽  
Galina Poljanskaya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Pluripotent Stem Cells ◽  
Expression Patterns ◽  
Embryonic Stem ◽  
Cancer Cell Lines ◽  
Cancer Testis Antigens ◽  
Hes Cells ◽  
Cancer Testis

Pluripotent stem cells can differentiate into various lineages but undergo genetic and epigenetic changes during long-term cultivation and, therefore, require regular monitoring. The expression patterns of cancer-testis antigens (CTAs) MAGE-A2, -A3, -A4, -A6, -A8, -B2, and GAGE were examined in undifferentiated human embryonic stem (hES) cells, their differentiated derivatives, teratocarcinoma (hEC) cells, and cancer cell lines of neuroectodermal and mesodermal origin. Undifferentiated hES cells and embryoid body cells expressed MAGE-A3, -A6, -A4, -A8, and GAGEs while later differentiated derivatives expressed only MAGE-A8 or MAGE-A4. Likewise, mouse pluripotent stem cells also express CTAs of Magea but not Mageb family. Despite similarity of the hES and hEC cell expression patterns, MAGE-A2 and MAGE-B2 were detected only in hEC cells but not in hES cells. Moreover, our analysis has shown that CTAs are aberrantly expressed in cancer cell lines and display low tissue specificity. The identification of CTA expression patterns in pluripotent stem cells and their derivatives may be useful for isolation of abnormally CTA-expressing cells to improve the safety of stem-cell based therapy.

scholarly journals Expression profiling of MMA-1a and splice variant MMA-1b: New cancer/testis antigens identified in human melanoma

2002 ◽  
Vol 98 (4) ◽  
pp. 547-553 ◽  
Author(s):  
Nicole J.W. de Wit ◽  
Ulrich H. Weidle ◽  
Dirk J. Ruiter ◽  
Goos N.P. van Muijen
Keyword(s):  
Expression Profiling ◽  
Splice Variant ◽  
Human Melanoma ◽  
Cancer Testis Antigens ◽  
Cancer Testis

scholarly journals Concise Review: Cancer/Testis Antigens, Stem Cells, and Cancer

Stem Cells ◽  
2006 ◽  
Vol 25 (3) ◽  
pp. 707-711 ◽  
Author(s):  
Fabrício F. Costa ◽  
Katarina Le Blanc ◽  
Bertha Brodin
Keyword(s):  
Stem Cells ◽  
Cancer Testis Antigens ◽  
Concise Review ◽  
Cancer Testis

scholarly journals Knockdown of Cancer Testis Antigens Modulates Neural Stem Cell Marker Expression in Glioblastoma Tumor Stem Cells

2010 ◽  
Vol 15 (7) ◽  
pp. 830-839 ◽  
Author(s):  
Jonathan Low ◽  
Michele Dowless ◽  
Tatiyana Shiyanova ◽  
Scott Rowlinson ◽  
Lucia Ricci-Vitiani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Morphological Changes ◽  
Gene Knockdown ◽  
Stem Cell Marker ◽  
Cell Marker ◽  
Tumor Stem Cells ◽  
Cancer Testis Antigens ◽  
Neural Stem Cell Marker ◽  
Cancer Testis

The cancer stem cell hypothesis posits that a subpopulation of cancer stem cells is frequently responsible for a tumor’s progression and resistance to treatment. The differential cellular morphology and gene expression between cancer stem cells and the majority of the tumor is becoming a point of attack for research into the next generation of therapeutic agents that may work through an induction of differentiation rather than apoptosis. Advances in the field of high-content imaging (HCI), combined with modern shRNA technology and subpopulation analysis tools, have created an ideal screening system to detect these morphological changes in a subset of cells upon gene knockdown. The authors examined several glioblastoma stem cell isolates pre- and postdifferentiation to elucidate the phenotypic effects caused by both serum differentiation and gene knockdown. Neural markers were first characterized in these cells at varying states of differentiation using HCI and immunoblots. The authors then chose one of these isolates, in both the pre- and postdifferentiated forms, for further analysis and screened for morphological changes upon shRNA knockdown of a panel of cancer testis antigens (CTAs). CTAs are a family of proteins that are normally expressed in male germ cells as well as heterogeneously expressed in some metastatic tumors. This gene family has also been implicated in the differentiation of normal human stem cells, therefore making it an ideal candidate for modulation in tumor stem cells. Using their approach, the authors identified the differential effects of gene knockdown in both cell types leading to either changes in neural stem cell marker expression or a decreased cell density likely due to growth arrest or cell death. The resolution that HCI brings to a screen at the subpopulation level makes it an excellent tool for the analysis of phenotypic changes induced by shRNA knockdown in a variety of tumor stem cells.

Cancer testis antigens and melanoma stem cells: new promises for therapeutic intervention

2009 ◽  
Vol 59 (3) ◽  
pp. 487-488 ◽  
Author(s):  
Luca Sigalotti ◽  
Alessia Covre ◽  
Hugues J. M. Nicolay ◽  
Sandra Coral ◽  
Michele Maio
Keyword(s):  
Stem Cells ◽  
Therapeutic Intervention ◽  
Cancer Testis Antigens ◽  
Cancer Testis

scholarly journals Expression of Cancer Testis Antigens in Tumor-Adjacent Normal Liver Is Associated with Post-Resection Recurrence of Hepatocellular Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2499
Author(s):  
Lisanne Noordam ◽  
Zhouhong Ge ◽  
Hadiye Özturk ◽  
Michail Doukas ◽  
Shanta Mancham ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Normal Liver ◽  
Occult Metastasis ◽  
Recurrence Rates ◽  
Curative Intent ◽  
Cancer Testis Antigens ◽  
Negative Prognostic Factor ◽  
Increased Risk ◽  
Hcc Recurrence ◽  
Cancer Testis

High recurrence rates after resection of hepatocellular carcinoma (HCC) with curative intent impair clinical outcomes of HCC. Cancer/testis antigens (CTAs) are suitable targets for cancer immunotherapy if selectively expressed in tumor cells. The aims were to identify CTAs that are frequently and selectively expressed in HCC-tumors, and to investigate whether CTAs could serve as biomarkers for occult metastasis. Tumor and paired tumor-free liver (TFL) tissues of HCC-patients and healthy tissues were assessed for mRNA expression of 49 CTAs by RT-qPCR and protein expression of five CTAs by immunohistochemistry. Twelve CTA-mRNAs were expressed in ≥10% of HCC-tumors and not in healthy tissues except testis. In tumors, mRNA and protein of ≥ 1 CTA was expressed in 78% and 71% of HCC-patients, respectively. In TFL, CTA mRNA and protein was found in 45% and 30% of HCC-patients, respectively. Interestingly, CTA-expression in TFL was an independent negative prognostic factor for post-resection HCC-recurrence and survival. We established a panel of 12 testis-restricted CTAs expressed in tumors of most HCC-patients. The increased risk of HCC-recurrence in patients with CTA expression in TFL, suggests that CTA-expressing (pre-)malignant cells may be a source of HCC-recurrence, reflecting the relevance of targeting these to prevent HCC-recurrence.

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