Synergistic effect of high glucose and ANG II on proliferation of mouse embryonic stem cells: Involvement of PKC and MAPKs as well as AT1 receptor

2008 ◽  
Vol 215 (2) ◽  
pp. 374-382 ◽  
Author(s):  
Yun Hee Kim ◽  
Ho Jae Han
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Synergistic Effect ◽  
High Glucose ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
At1 Receptor ◽  
Ang Ii

ANG II increases 2-deoxyglucose uptake in mouse embryonic stem cells

Life Sciences ◽  
2005 ◽  
Vol 77 (15) ◽  
pp. 1916-1933 ◽  
Author(s):  
Ho Jae Han ◽  
Jung Sun Heo ◽  
Yun Jung Lee
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Ang Ii

scholarly journals Nature of glycosylphosphatidylinositols produced by mouse embryonic stem cells

Reproduction ◽  
2001 ◽  
pp. 785-791 ◽  
Author(s):  
LR Quinlan ◽  
MT Kane
Keyword(s):  
Signal Transduction ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Glucose ◽  
Glucose Concentration ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
High Glucose Concentration ◽  
Unknown Lipid ◽  
Phosphatidylinositol 4

Incorporation of [(3)H]inositol into mouse embryonic stem cells of the CCE cell line leads to the labelling of the three common phosphoinositides, phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, and a fourth unknown lipid (lipid X). Incubation with [(3)H]glucosamine results in the labelling of lipid X and at least one other lipid that co-migrates with phosphatidylinositol (lipid Y), indicating that both of these lipids are putative glycosylphosphatidylinositols. In this study, the incorporation of other possible glycosylphosphatidylinositol precursors, ethanolamine, mannose and galactose, into lipids X and Y was examined. Galactose was incorporated into lipids X and Y, and ethanolamine and mannose into lipid Y only. Inhibitors of glycosylphosphatidylinositol biosynthesis pathways, mannosamine and 2-fluoro-2-deoxyglucose, both significantly inhibited ethanolamine incorporation into lipid Y. A high glucose concentration (25 mmol l(-1)) abolished the action of both inhibitors. Phospholipase C treatment of embryonic stem cells that had been labelled in culture with [(3)H]ethanolamine caused a large release of ethanolamine label into the incubation medium and markedly decreased the amount of ethanolamine-labelled lipid Y remaining in the cell membranes. These effects were almost totally abolished by incubation with mannosamine before ethanolamine labelling. These studies strongly indicate that lipid Y is a member of the protein anchor class of glycosylphosphatidylinositol, whereas lipid X is a member of the signal transduction inositol phosphoglycan class of glycosylphosphatidylinositol.

Sign in / Sign up

Export Citation Format

Share Document