Long-term cryopreservation of dental pulp stem cells (SBP-DPSCs) and their differentiated osteoblasts: A cell source for tissue repair

2006 ◽  
Vol 208 (2) ◽  
pp. 319-325 ◽  
Author(s):  
Gianpaolo Papaccio ◽  
Antonio Graziano ◽  
Riccardo d'Aquino ◽  
Maria Francesca Graziano ◽  
Giuseppe Pirozzi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Tissue Repair ◽  
Dental Pulp Stem Cells ◽  
Cell Source ◽  
A Cell

scholarly journals Dental pulp stem cells can improve muscle dysfunction in animal models of Duchenne muscular dystrophy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuko Nitahara-Kasahara ◽  
Mutsuki Kuraoka ◽  
Posadas Herrera Guillermo ◽  
Hiromi Hayashita-Kinoh ◽  
Yasunobu Maruoka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Dental Pulp ◽  
Systemic Treatment ◽  
Dental Pulp Stem Cells ◽  
Mri Findings ◽  
Systemic Injection ◽  
Therapeutic Benefits

Abstract Background Duchenne muscular dystrophy (DMD) is an inherited progressive disorder that causes skeletal and cardiac muscle deterioration with chronic inflammation. Dental pulp stem cells (DPSCs) are attractive candidates for cell-based strategies for DMD because of their immunosuppressive properties. Therefore, we hypothesized that systemic treatment with DPSCs might show therapeutic benefits as an anti-inflammatory therapy. Methods To investigate the potential benefits of DPSC transplantation for DMD, we examined disease progression in a DMD animal model, mdx mice, by comparing them with different systemic treatment conditions. The DPSC-treated model, a canine X-linked muscular dystrophy model in Japan (CXMDJ), which has a severe phenotype similar to that of DMD patients, also underwent comprehensive analysis, including histopathological findings, muscle function, and locomotor activity. Results We demonstrated a therapeutic strategy for long-term functional recovery in DMD using repeated DPSC administration. DPSC-treated mdx mice and CXMDJ showed no serious adverse events. MRI findings and muscle histology suggested that DPSC treatment downregulated severe inflammation in DMD muscles and demonstrated a milder phenotype after DPSC treatment. DPSC-treated models showed increased recovery in grip-hand strength and improved tetanic force and home cage activity. Interestingly, maintenance of long-term running capability and stabilized cardiac function was also observed in 1-year-old DPSC-treated CXMDJ. Conclusions We developed a novel strategy for the safe and effective transplantation of DPSCs for DMD recovery, which included repeated systemic injection to regulate inflammation at a young age. This is the first report on the efficacy of a systemic DPSC treatment, from which we can propose that DPSCs may play an important role in delaying the DMD disease phenotype.

scholarly journals Harvesting human dental pulp stem cells using long term ex-plant culture

2019 ◽  
Vol 8 (1) ◽  
pp. 1-2
Author(s):  
Vikrant R Patil ◽  
Avinash H Kharat ◽  
Supriya Kheur ◽  
Mahesh Chavan ◽  
D Gopalakrishnan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Plant Culture ◽  
Human Dental Pulp

scholarly journals Pentraxin-3 Modulates Osteogenic/Odontogenic Differentiation and Migration of Human Dental Pulp Stem Cells

2019 ◽  
Vol 20 (22) ◽  
pp. 5778
Author(s):  
Yeon Kim ◽  
Joo-Yeon Park ◽  
Hyun-Joo Park ◽  
Mi-Kyoung Kim ◽  
Yong-Il Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Tissue Repair ◽  
Therapeutic Potential ◽  
Dental Pulp Stem Cells ◽  
Pentraxin 3 ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
And Migration

Pentraxin-3 (PTX3) is recognized as a modulator of inflammation and a mediator of tissue repair. In this study, we characterized the role of PTX3 on some biological functions of human dental pulp stem cells (HDPSCs). The expression level of PTX3 significantly increased during osteogenic/odontogenic differentiation of HDPSCs, whereas the knockdown of PTX3 decreased this differentiation. Silencing of PTX3 in HDPSCs inhibited their migration and C-X-C chemokine receptor type 4 (CXCR4) expression. Our present study indicates that PTX3 is involved in osteogenic/odontogenic differentiation and migration of HDPSCs, and may contribute to the therapeutic potential of HDPSCs for regeneration and repair.

scholarly journals THE EFFECT OF FETAL CALF SERUM ON HUMAN DENTAL PULP STEM CELLS

2013 ◽  
Vol 56 (4) ◽  
pp. 142-149 ◽  
Author(s):  
Jakub Suchánek ◽  
Tereza Suchánková Kleplová ◽  
Martin Kapitán ◽  
Tomáš Soukup
Keyword(s):  
Stem Cells ◽  
Side Effects ◽  
Chromosomal Aberrations ◽  
Dental Pulp ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Dental Pulp Stem Cells ◽  
Proliferation Rate ◽  
Human Dental Pulp

Aims: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long term cultivation. Methods: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. Results: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2% FCS without ITS supplement was slowest. Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not seem to be clonal but surprisingly we found large amounts of tetraploid cells in the 9th passage in both media containing 10% FCS. Conclusions: Our study proved that cultivation of DPSC in media containing higher concentration of FCS has critical side effects on cell chromosomal stability.

Long term explant culture for harvesting homogeneous population of human dental pulp stem cells

2018 ◽  
Vol 42 (12) ◽  
pp. 1602-1610
Author(s):  
Vikrant R. Patil ◽  
Avinash H. Kharat ◽  
Deepak G. Kulkarni ◽  
Supriya M. Kheur ◽  
Ramesh R. Bhonde
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Explant Culture ◽  
Dental Pulp Stem Cells ◽  
Homogeneous Population ◽  
Human Dental Pulp

Micromanipulation of culture niche permits long-term expansion of dental pulp stem cells—an economic and commercial angle

2010 ◽  
Vol 46 (9) ◽  
pp. 764-773 ◽  
Author(s):  
Vijayendran Govindasamy ◽  
Veronica Sainik Ronald ◽  
Swapnil Totey ◽  
Salina Binti Din ◽  
Wan Mahadzir Bin Wan Mustafa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells

scholarly journals Human dental pulp stem cells: a sanctuary for relapsing Bartonella quintana

2020 ◽  
Author(s):  
Hamadou Oumarou Hama ◽  
Attoumani Hamada ◽  
Gérard Aboudharam ◽  
Éric Ghigo ◽  
Michel Drancourt
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Bacterial Load ◽  
Dental Pulp Stem Cells ◽  
Bartonella Quintana ◽  
Clinical Investigations ◽  
Produce Tumor Necrosis Factor ◽  
Human Dental Pulp ◽  
Factor Α

ABSTRACTBartonella quintana is a facultative intracellular bacterium responsible for relapsing fever, an example of non-sterilizing immunity. The cellular sanctuary of B. quintana in-between febrile relapses remains unknown but repeated detection of B. quintana in dental pulp specimens suggested long-term half-life dental pulp stem cells (DPSCs) as candidates. As the capacity of DPSCs to internalize microscopic particles was unknown, we confirmed that DPSCs internalized B. quintana bacteria: Gimenez staining and fluorescence microscopy localized B. quintana bacteria inside DPSCs and this internalization did not affect the cellular multiplication of DPSCs during a one-month follow-up despite the increase in the bacterial load. B. quintana-infected DPSCs did not produce Tumor Necrosis Factor-α whereas an important production of Monocytes Chemoattractant Protein-1 was observed. These unprecedented observations suggested the possibility that DPSCs were shelters for the long-term persistence of B. quintana in the host, warranting further experimental and clinical investigations.

Human Dental Pulp Stem Cells. A promising epithelial-like cell source

2015 ◽  
Vol 84 (5) ◽  
pp. 516-517 ◽  
Author(s):  
I. Garzón ◽  
M.A. Martin-Piedra ◽  
M. Alaminos
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Cell Source ◽  
Human Dental Pulp
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