COP-1, a member of the CCN family, is a heparin-induced growth arrest specific gene in vascular smooth muscle cells

2001 ◽  
Vol 188 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Laurie M. Delmolino ◽  
Nancy A. Stearns ◽  
John J. Castellot
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Growth Arrest ◽  
Muscle Cells ◽  
Specific Gene ◽  
Ccn Family

scholarly journals A product of growth arrest-specific gene 6 modulates scavenger receptor expression in human vascular smooth muscle cells

FEBS Letters ◽  
1999 ◽  
Vol 459 (3) ◽  
pp. 363-366 ◽  
Author(s):  
Koji Murao ◽  
Hitomi Imachi ◽  
Yoshitaka Sayo ◽  
Hitoshi Hosokawa ◽  
Makoto Sato ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Scavenger Receptor ◽  
Growth Arrest ◽  
Muscle Cells ◽  
Receptor Expression ◽  
Specific Gene

The anticoagulant factor, protein S, is produced by cultured human vascular smooth muscle cells and its expression is up-regulated by thrombin

Blood ◽  
2000 ◽  
Vol 95 (6) ◽  
pp. 2008-2014 ◽  
Author(s):  
Omar Benzakour ◽  
Chryso Kanthou
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Vitamin K ◽  
Messenger Rna ◽  
Growth Arrest ◽  
Protein S ◽  
Muscle Cells

Abstract The anticoagulant factor protein S is a secreted vitamin K-dependent γ-carboxylated protein that is mainly made in the liver. Protein S is homologous to the growth arrest specific protein, Gas6, the expression of which is up-regulated in cultured fibroblasts upon serum withdrawal. We report here the synthesis and secretion of protein S by cultured human vascular smooth muscle cells (HVSMCs). Western blot analysis revealed that similar amounts of protein S are secreted by both growing and growth-arrested HVSMCs. HVSMC-derived protein S was found to be γ-carboxylated as it was precipitated by barium citrate and was shown to possess protein C cofactor activity. Treatment with the vitamin K antagonist warfarin led to the accumulation of intracellular undercarboxylated protein S forms that were rapidly secreted upon the reintroduction of vitamin K. Northern blotting analysis showed that cultured HVSMCs express a protein S transcript. The expression of protein S messenger RNA was unaffected by either warfarin, growth arrest, or various VSMC mitogens, such as platelet-derived growth factor-BB, basic fibroblast growth factor, transforming growth factor-β, or hepatocyte growth factor. Thrombin, however, induced an up-regulation of protein S expression at both messenger RNA and protein levels. The evidence we provide for protein S secretion by cultured HVSMCs and its up-regulation by thrombin, together with earlier reports showing that protein S acts as a mitogen for these cells, suggests that, in addition to its known role in regulating blood clotting, protein S may also be an important autocrine factor in the pathophysiology of the vasculature.

Growth arrest of vascular smooth muscle cells in suspension culture using low-acyl gellan gum

2016 ◽  
Vol 53 (3) ◽  
pp. 191-198 ◽  
Author(s):  
Tomomi Natori ◽  
Masachika Fujiyoshi ◽  
Masashi Uchida ◽  
Natsuki Abe ◽  
Tatsuro Kanaki ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Suspension Culture ◽  
Vascular Smooth Muscle Cells ◽  
Growth Arrest ◽  
Muscle Cells ◽  
Gellan Gum
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