Synergistic interaction of interleukin-1? and growth factors in primary cultures of rat aortic smooth muscle cells

1995 ◽  
Vol 164 (3) ◽  
pp. 644-657 ◽  
Author(s):  
Todd Bourcier ◽  
Michael Dockter ◽  
Aviv Hassid
Keyword(s):  
Smooth Muscle ◽  
Growth Factors ◽  
Smooth Muscle Cells ◽  
Interleukin 1 ◽  
Primary Cultures ◽  
Muscle Cells ◽  
Synergistic Interaction ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Phenotype modulation in primary cultures of aortic smooth muscle cells from streptozotocin-diabetic rats

1998 ◽  
Vol 63 (4) ◽  
pp. 225-236 ◽  
Author(s):  
Pascale Etienne ◽  
Núria Parés-Herbuté ◽  
Louis Monnier ◽  
Herisoa Rabesandratana ◽  
Laurence Mani-Ponset ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Primary Cultures ◽  
Muscle Cells ◽  
Diabetic Rats ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Streptozotocin Diabetic Rats

Density-dependent hyperpolarization in cultured aortic smooth muscle cells

1989 ◽  
Vol 256 (3) ◽  
pp. C644-C651 ◽  
Author(s):  
M. G. Blennerhassett ◽  
M. S. Kannan ◽  
R. E. Garfield
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Continuous Process ◽  
Primary Cultures ◽  
Muscle Cells ◽  
Sprague Dawley ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Wistar Kyoto

The membrane potential (Em) of cultured aortic smooth muscle cells from Sprague-Dawley (SD), Wistar-Kyoto (WKY), and spontaneously hypertensive (SHR) rats was measured in proliferating primary cultures. Em of SD cells in high-density cultures was -51 to -58 mV, whereas that of low-density cultures (1-2 days) was -30 mV. This difference was due to a continuous process of hyperpolarization during proliferation in culture. Em of WKY and SHR hyperpolarized similarly, from -12 to -42 and -38 mV, respectively. Hyperpolarization of Em of SD, WKY, and SHR cells was related to cell density rather than time in culture. Em may be a sensitive and significant indicator of the changes in the differentiated state expressed by proliferating smooth muscle in vitro.

scholarly journals Inhibitory effects of cnidium components on proliferation of mouse aortic smooth muscle cells in primary cultures

1988 ◽  
Vol 46 ◽  
pp. 185
Author(s):  
Masayasu Kimura ◽  
Shinjiro Kobayashi ◽  
Kouhei Notoya ◽  
Yasuhiko Mimura ◽  
Jun Suzuki ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Primary Cultures ◽  
Muscle Cells ◽  
Inhibitory Effects ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells

1994 ◽  
Vol 267 (6) ◽  
pp. H2114-H2123 ◽  
Author(s):  
C. Koncz ◽  
J. T. Daugirdas
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Primary Cultures ◽  
Chloride Concentration ◽  
Maximum Velocity ◽  
Muscle Cells ◽  
Disulfonic Acid ◽  
Influx Rate ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

A novel fluorescent indicator, N-[ethoxycarbonylmethyl]-6-methoxy-quinolinium bromide (MQAE), was used to measure intracellular chloride concentration ([Cl-]i) in primary cultures of rat aortic smooth muscle cells (VSMC). The hydrolytic and fluorescent properties of the dye were characterized. The intracellular Stern-Volmer constant was calculated to be 25 M-1. Cl- efflux curves were characteristic of saturation-type kinetics, with an apparent Michaelis-Menten constant value of 11 +/- 4.8 (SD) mM, a maximum velocity of 0.038 +/- 0.021 mM/s, and a half time (t1/2) of 9.0 +/- 3.7 min. The average efflux rate in the first 10 min (0.023 +/- 0.004 mM/s) was reduced in the presence of either 130 microM 4,4'-diisothiocyanato-dihydrostilbene-2,2'-disulfonic acid (H2DIDS) (0.014 +/- 0.006, P = 0.02) or 40 microM furosemide (0.017 +/- 0.004, P = 0.04). Restoration of physiological extracellular chloride concentration ([Cl-]o) after zero Cl- resulted in net Cl- influx with a t1/2 of 3.6 +/- 1.0 min. The initial Cl- influx rate was reduced after exposure to furosemide, from 0.069 +/- 0.006 to 0.046 +/- 0.008 mM/s, P < 0.002, and was reduced after exposure to H2DIDS from 0.102 +/- 0.013 to 0.033 +/- 0.003 mM/s, P < 0.001. Furosemide reduced the steady-state [Cl-]i from 31.6 +/- 3.2 to 26.1 +/- 2.4 mM, P < 0.01, whereas H2DIDS had little effect on [Cl-]i. Our results demonstrate that MQAE can be used to measure [Cl-]i in primary cultures of VSMC.

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