The proto-oncogene C-myc is involved in cell differentiation as well as cell proliferation: Studies on growth plate chondrocytes in situ

1992 ◽  
Vol 152 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Colin Farquharson ◽  
John E. Hesketh ◽  
Nigel Loveridge
Keyword(s):  
Cell Proliferation ◽  
Cell Differentiation ◽  
Growth Plate ◽  
Growth Plate Chondrocytes

In situ deformation of growth plate chondrocytes in stress-controlled static vs dynamic compression

2017 ◽  
Vol 56 ◽  
pp. 76-82 ◽  
Author(s):  
Elizabeth A. Zimmermann ◽  
Séréna Bouguerra ◽  
Irene Londoño ◽  
Florina Moldovan ◽  
Carl-Éric Aubin ◽  
...  
Keyword(s):  
Growth Plate ◽  
Dynamic Compression ◽  
Growth Plate Chondrocytes ◽  
In Situ Deformation

scholarly journals Type B γ-Aminobutyric Acid Receptors Modulate the Function of the Extracellular Ca2+-Sensing Receptor and Cell Differentiation in Murine Growth Plate Chondrocytes

Endocrinology ◽  
2007 ◽  
Vol 148 (10) ◽  
pp. 4984-4992 ◽  
Author(s):  
Zhiqiang Cheng ◽  
Chialing Tu ◽  
Luis Rodriguez ◽  
Tsui-Hua Chen ◽  
Melita M. Dvorak ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Growth Plate ◽  
Type Ii Collagen ◽  
Aminobutyric Acid ◽  
Physical Interaction ◽  
Type B ◽  
Growth Plate Chondrocytes ◽  
Differentiation Markers ◽  
Receptor Complexes

Extracellular calcium-sensing receptors (CaRs) and metabotropic or type B γ-aminobutyric acid receptors (GABA-B-Rs), two closely related members of family C of the G protein-coupled receptor superfamily, dimerize in the formation of signaling and membrane-anchored receptor complexes. We tested whether CaRs and two GABA-B-R subunits (R1 and R2) are expressed in mouse growth plate chondrocytes (GPCs) by PCR and immunocytochemistry and whether interactions between these receptors influence the expression and function of the CaR and extracellular Ca2+-mediated cell differentiation. Both CaRs and the GABA-B-R1 and -R2 were expressed in the same zones of the growth plate and extensively colocalized in intracellular compartments and on the membranes of cultured GPCs. The GABA-B-R1 coimmunoprecipitated with the CaR, confirming a physical interaction between the two receptors in GPCs. In vitro knockout of GABA-B-R1 genes, using a Cre-lox recombination strategy, blunted the ability of high extracellular Ca2+ concentration to activate phospholipase C and ERK1/2, suppressed cell proliferation, and enhanced apoptosis in cultured GPCs. In GPCs, in which the GABA-B-R1 was acutely knocked down, there was reduced expression of early chondrocyte markers, aggrecan and type II collagen, and increased expression of the late differentiation markers, type X collagen and osteopontin. These results support the idea that physical interactions between CaRs and GABA-B-R1s modulate the growth and differentiation of GPCs, potentially by altering the function of CaRs.

scholarly journals Defined Carboxy-Terminal Fragments of Insulin-Like Growth Factor (IGF) Binding Protein-2 Exert Similar Mitogenic Activity on Cultured Rat Growth Plate Chondrocytes as IGF-I

Endocrinology ◽  
2008 ◽  
Vol 149 (10) ◽  
pp. 4901-4911 ◽  
Author(s):  
Daniela Kiepe ◽  
Anke Van Der Pas ◽  
Sonia Ciarmatori ◽  
Ludger Ständker ◽  
Burkhardt Schütt ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Biological Activity ◽  
Growth Plate ◽  
Longitudinal Growth ◽  
Growth Plate Chondrocytes ◽  
Igf I ◽  
Rat Growth ◽  
Igf Binding ◽  
Carboxy Terminal ◽  
Igf Binding Protein

The IGF/IGF binding protein (IGFBP) system is an important component in the hormonal regulation of longitudinal growth. Evidence from in vitro studies indicates that IGFBPs may have IGF-independent effects. We analyzed the biological activity of intact IGFBP-2 and defined carboxy-terminal IGFBP-2 fragments isolated from human hemofiltrate in two cell culture systems of the growth plate: rat growth plate chondrocytes in primary culture and the mesenchymal chondrogenic cell line RCJ3.1C5.18. The IGFBP-2 fragments IGFBP-2167–279, IGFBP-2167–289, and IGFBP-2104–289 exerted a strong (2- to 3-fold) mitogenic effect on growth plate chondrocytes, which was comparable with IGF-I in equimolar concentrations (7.8 nm) but was not mediated through the type 1 IGF receptor. In a dose-response experiment, the most effective concentration of IGFBP-2104–289 for the stimulation of cell proliferation was 10 nm. This biological activity of IGFBP-2 fragments was associated with cell membrane binding, demonstrated by Western blot analysis of fractionated cell lysates and immunohistochemistry. Whereas intact IGFBP-2 did not modulate chondrocyte proliferation, partially reduced (by dithiothreitol) full-length IGFBP-2 stimulated cell proliferation to a comparable extent (3.4-fold) as carboxy-terminal IGFBP-2 fragments. The mitogenic activity of these IGFBP-2 fragments and of partially reduced full-length IGFBP-2 was mediated through the use of the MAPK/ERK 1/2. These data imply a novel role of naturally occurring IGFBP-2 fragments for the endocrine and paracrine/autocrine regulation of longitudinal growth.

Cell differentiation alters metallothionein expression in avian growth plate chondrocytes

1999 ◽  
pp. 333-338
Author(s):  
Glenn R. Sauer ◽  
Licia N. Y. Wu ◽  
Daotai Nie ◽  
Roy E. Wuthier
Keyword(s):  
Cell Differentiation ◽  
Growth Plate ◽  
Growth Plate Chondrocytes
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