Verapamil inhibits serotonin uptake of endothelial cells in culture by a mechanism unrelated to Ca2+ channel blockade

1987 ◽  
Vol 132 (1) ◽  
pp. 178-182 ◽  
Author(s):  
S.-L. Lee ◽  
K. Long ◽  
S. Ueda ◽  
B. L. Fanburg
Keyword(s):  
Endothelial Cells ◽  
Serotonin Uptake ◽  
Channel Blockade ◽  
Cells In Culture

Serotonin uptake by bovine pulmonary artery endothelial cells in culture. I. Characterization

1986 ◽  
Vol 250 (5) ◽  
pp. C761-C765 ◽  
Author(s):  
S. L. Lee ◽  
B. L. Fanburg
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Serotonin Uptake ◽  
High Affinity ◽  
Total Uptake ◽  
Cells In Culture ◽  
High Concentrations ◽  
5 Ht Uptake

Serotonin (5-HT) uptake by bovine pulmonary artery endothelial cells in culture was measured at 5-HT concentrations of 1.5 X 10(-8) to 10(-3) M. The uptake increased linearly at concentrations of 10(-5) to 10(-3) M 5-HT, and the slope for uptake was 1.276 X 10(6) pmol 5-HT X 10(6) cells-1 X 30 min-1 X M concentration-1. The slope for uptake at 5-HT concentrations less than 10(-7) M was 7.10 X 10(6) pmol X 10(6) cells-1 X 30 min-1 X M concentration-1. With subtraction of the slope for linear uptake obtained at high concentrations of 5-HT from total uptake of 5-HT, a curve showing saturable uptake at concentrations greater than 10(-6) to 10(-5) M was obtained. Lineweaver-Burk analysis of the data yielded a saturated value of high-affinity transport of 6.25 pmol X 10(6) cells-1 X 30 min-1 and a Km of 10(-6) M. At 5-HT concentrations of 1.5 X 10(-8) to 5 X 10(-7) M, uptake was inhibited 75-80% by exposure to 4 degrees C or by absence of sodium in the medium; absence of sodium did not affect uptake at 5-HT concentrations greater than 10(-5) M, and 4 degrees C was less effective in inhibiting uptake at high 5-HT concentrations. Imipramine markedly inhibited uptake at low, but not at high, 5-HT concentrations. Ouabain only partially inhibited 5-HT uptake, but inhibition was more pronounced at low 5-HT concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)

Human Fetal Endothelial Cells in Culture

2009 ◽  
Vol 14 (4) ◽  
pp. 233-241 ◽  
Author(s):  
Tore Henriksen ◽  
Stein A. Evensen ◽  
Ragna Følling Elgjo ◽  
Anne Vefling
Keyword(s):  
Endothelial Cells ◽  
Cells In Culture

ENDOTHELIAL CELLS IN CULTURE: A MODEL FOR STUDYING VASCULAR FUNCTIONS

2000 ◽  
Vol 42 (1) ◽  
pp. 9-19 ◽  
Author(s):  
TIZIANA BACHETTI ◽  
LUCIA MORBIDELLI
Keyword(s):  
Endothelial Cells ◽  
Cells In Culture
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