Possible involvement of reorganization of actin filaments, induced by tumor-promoting phorbol esters, in changes in colony shape and enhancement of proliferation of cultured epithelial cells

1987 ◽  
Vol 132 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Sumadhi Sastrodihardjo ◽  
Yasuto Sasaki ◽  
Yoshiki Shiba ◽  
Yoshinobu Kanno
Keyword(s):  
Epithelial Cells ◽  
Actin Filaments ◽  
Phorbol Esters ◽  
Cultured Epithelial Cells ◽  
Colony Shape

scholarly journals Signals from the spindle midzone are required for the stimulation of cytokinesis in cultured epithelial cells.

1996 ◽  
Vol 7 (2) ◽  
pp. 225-232 ◽  
Author(s):  
L G Cao ◽  
Y L Wang
Keyword(s):  
Epithelial Cells ◽  
Mitotic Spindle ◽  
Actin Filaments ◽  
Cultured Cells ◽  
Critical Role ◽  
Equatorial Region ◽  
Culture Cells ◽  
Cultured Epithelial Cells ◽  
Early Anaphase ◽  
Spindle Midzone

The interaction between the mitotic spindle and the cellular cortex is thought to play a critical role in stimulating cell cleavage. However, little is understood about the nature of such interactions, particularly in tissue culture cells. We have investigated the role of the spindle midzone in signaling cytokinesis by creating a barrier in cultured epithelial cells with a blunted needle, to block signals that may emanate from this region. When the barrier was created during metaphase or early anaphase, cleavage took place only on the sides of the cortex facing the mitotic spindle. Microtubules on the cleaving side showed organization typical of that in normal dividing cells. On the noncleaving side, most microtubules passed from one side of the equator into the other without any apparent organization, and actin filaments failed to organize in the equatorial region. When the barrier was created after the first minute of anaphase, cells showed successful cytokinesis, with normal organization of microtubules and actin filaments on both sides of the barrier. Our study suggests that transient signals from the midzone of early anaphase spindles are required for equatorial contraction in cultured cells and that such signaling may involve the organization of microtubules near the equator.

Vimentin organizing centre in cultured epithelial cells

Pathology ◽  
1984 ◽  
Vol 16 (4) ◽  
pp. 393-395 ◽  
Author(s):  
John S. Pedersen ◽  
J.R. Underwood ◽  
B.H. Toh
Keyword(s):  
Epithelial Cells ◽  
Cultured Epithelial Cells

Cultured epithelial cells derived from human foetal pancreas as a model for the study of cystic fibrosis: further analyses on the origins and nature of the cell types

1988 ◽  
Vol 90 (1) ◽  
pp. 73-77
Author(s):  
A. Harris ◽  
L. Coleman
Keyword(s):  
Cystic Fibrosis ◽  
Tissue Culture ◽  
Epithelial Cells ◽  
Culture System ◽  
Cultured Cells ◽  
Cell Types ◽  
Cultured Epithelial Cells ◽  
Different Cell Types ◽  
Foetal Pancreas

The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results support the view that the cultured cells are ductal in origin and nature. Pancreatic epithelial cell cultures have also been established from foetuses with cystic fibrosis.

Drug-induced alterations of cytokeratin organization in cultured epithelial cells

Science ◽  
1983 ◽  
Vol 219 (4584) ◽  
pp. 501-503 ◽  
Author(s):  
L. Knapp ◽  
W. O'Guin ◽  
R. Sawyer
Keyword(s):  
Epithelial Cells ◽  
Drug Induced ◽  
Cultured Epithelial Cells

scholarly journals Expression of Matrix Metalloproteinase Gelatinases A and B by Cultured Epithelial Cells from Human Bronchial Explants

1996 ◽  
Vol 271 (26) ◽  
pp. 15580-15589 ◽  
Author(s):  
Pin Mei Yao ◽  
Jean-Marie Buhler ◽  
Marie Pia d'Ortho ◽  
François Lebargy ◽  
Christophe Delclaux ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Matrix Metalloproteinase ◽  
Cultured Epithelial Cells ◽  
Gelatinases A And B
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