Characterization of mouse lymphoma cells with altered nucleoside transport

1985 ◽  
Vol 123 (3) ◽  
pp. 431-434 ◽  
Author(s):  
Amos Cohen ◽  
Changyee Leung ◽  
Ellen Thompson
Keyword(s):  
Nucleoside Transport ◽  
Lymphoma Cells ◽  
Mouse Lymphoma Cells ◽  
Mouse Lymphoma

Characterization of Thymidine Kinase in L5178y mOuse Lymphoma Cells

1985 ◽  
Vol 1 (3) ◽  
pp. 1-12 ◽  
Author(s):  
Kenneth A. Palmer ◽  
Rosalie De Giovanni-Donnelly
Keyword(s):  
Thymidine Kinase ◽  
Lymphoma Cells ◽  
Mouse Lymphoma Cells ◽  
Mouse Lymphoma

scholarly journals Nucleoside transport in Walker 256 rat carcinosarcoma and S49 mouse lymphoma cells. Differences in sensitivity to nitrobenzylthioinosine and thiol reagents

1985 ◽  
Vol 232 (3) ◽  
pp. 681-688 ◽  
Author(s):  
J A Belt ◽  
L D Noel
Keyword(s):  
Diffusion Mechanism ◽  
Nucleoside Transport ◽  
Facilitated Diffusion ◽  
Lymphoma Cells ◽  
Transport Inhibitor ◽  
Mouse Lymphoma Cells ◽  
Thiol Reagent ◽  
Walker 256 ◽  
Nucleoside Transport Inhibitor ◽  
Mouse Lymphoma

The characteristics of nucleoside transport were examined in Walker 256 rat carcinosarcoma and S49 mouse lymphoma cells. In Walker 256 cells the initial rates of uridine, thymidine and adenosine uptake were insensitive to the nucleoside transport inhibitor nitrobenzylthioinosine (NBMPR) (1 microM), but were partially inhibited by dipyridamole (10 microM), another inhibitor of nucleoside transport. In contrast, the transport of these nucleosides in S49 cells was completely blocked by both inhibitors. Nucleoside transport in Walker 256 and S49 cells also differed in its sensitivity to the thiol reagent p-chloromercuribenzenesulphonate (pCMBS). Uridine transport in Walker 256 cells was inhibited by pCMBS with an IC50 (concentration producing 50% inhibition) of less than 25 microM, and inhibition was readily reversed by beta-mercaptoethanol. In S49 cells uridine transport was only inhibited at much higher concentrations of pCMBS (IC50 approximately equal to 300 microM). In other respects nucleoside transport in Walker 256 and S49 cells were quite similar. The Km and Vmax. values for uridine transport were nearly identical, and the transporters of both cell lines appeared to accept a broad range of nucleosides as substrates. Uridine transport in Walker 256 cells was non-concentrative and did not require an energy source. These studies demonstrate that nucleoside uptake in Walker 256 cells is mediated by a facilitated-diffusion mechanism which differs markedly from that of S49 cells in its sensitivity to the transport inhibitor NBMPR and the thiol reagent pCMBS.

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