Effects of peripheral benzodiazepine receptor ligands on proliferation and differentiation of human mesenchymal stem cells

2003 ◽  
Vol 198 (1) ◽  
pp. 91-99 ◽  
Author(s):  
D.H. Lee ◽  
S.K. Kang ◽  
R.H. Lee ◽  
J.M. Ryu ◽  
H.Y. Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Benzodiazepine Receptor ◽  
Peripheral Benzodiazepine Receptor ◽  
Receptor Ligands ◽  
Benzodiazepine Receptor Ligands ◽  
Proliferation And Differentiation

scholarly journals Midazolam inhibits chondrogenesis via peripheral benzodiazepine receptor in human mesenchymal stem cells

2018 ◽  
Vol 22 (5) ◽  
pp. 2896-2907 ◽  
Author(s):  
Yung-Ching Chen ◽  
King-Chuen Wu ◽  
Bu-Miin Huang ◽  
Edmund Cheung So ◽  
Yang-Kao Wang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Benzodiazepine Receptor ◽  
Peripheral Benzodiazepine Receptor

scholarly journals Fluid Flow Mechanical Stimulation-Assisted Cartridge Device for the Osteogenic Differentiation of Human Mesenchymal Stem Cells

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 927
Author(s):  
Ki-Taek Lim ◽  
Dinesh-K. Patel ◽  
Sayan-Deb Dutta ◽  
Keya Ganguly
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluid Flow ◽  
Osteogenic Differentiation ◽  
Flow Condition ◽  
Cultured Cells ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation ◽  
Static Conditions

Human mesenchymal stem cells (hMSCs) have the potential to differentiate into different types of mesodermal tissues. In vitro proliferation and differentiation of hMSCs are necessary for bone regeneration in tissue engineering. The present study aimed to design and develop a fluid flow mechanically-assisted cartridge device to enhance the osteogenic differentiation of hMSCs. We used the fluorescence-activated cell-sorting method to analyze the multipotent properties of hMSCs and found that the cultured cells retained their stemness potential. We also evaluated the cell viabilities of the cultured cells via water-soluble tetrazolium salt 1 (WST-1) assay under different rates of flow (0.035, 0.21, and 0.35 mL/min) and static conditions and found that the cell growth rate was approximately 12% higher in the 0.035 mL/min flow condition than the other conditions. Moreover, the cultured cells were healthy and adhered properly to the culture substrate. Enhanced mineralization and alkaline phosphatase activity were also observed under different perfusion conditions compared to the static conditions, indicating that the applied conditions play important roles in the proliferation and differentiation of hMSCs. Furthermore, we determined the expression levels of osteogenesis-related genes, including the runt-related protein 2 (Runx2), collagen type I (Col1), osteopontin (OPN), and osteocalcin (OCN), under various perfusion vis-à-vis static conditions and found that they were significantly affected by the applied conditions. Furthermore, the fluorescence intensities of OCN and OPN osteogenic gene markers were found to be enhanced in the 0.035 mL/min flow condition compared to the control, indicating that it was a suitable condition for osteogenic differentiation. Taken together, the findings of this study reveal that the developed cartridge device promotes the proliferation and differentiation of hMSCs and can potentially be used in the field of tissue engineering.

The metabolism of human mesenchymal stem cells during proliferation and differentiation

2011 ◽  
Vol 226 (10) ◽  
pp. 2562-2570 ◽  
Author(s):  
Girish Pattappa ◽  
Hannah K. Heywood ◽  
Joost D. de Bruijn ◽  
David A. Lee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation

2-Arylpyrazolo[1,5-a]pyrimidin-3-yl acetamides. New potent and selective peripheral benzodiazepine receptor ligands

2001 ◽  
Vol 9 (10) ◽  
pp. 2661-2671 ◽  
Author(s):  
Silvia Selleri ◽  
Fabrizio Bruni ◽  
Camilla Costagli ◽  
Annarella Costanzo ◽  
Gabriella Guerrini ◽  
...  
Keyword(s):  
Benzodiazepine Receptor ◽  
Peripheral Benzodiazepine Receptor ◽  
Receptor Ligands ◽  
Benzodiazepine Receptor Ligands
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