Characterization of apoptosis signal transduction pathways in HL-5 cardiomyocytes exposed to ischemia/reperfusion oxidative stress model

2003 ◽  
Vol 195 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Simona Cicconi ◽  
Natascia Ventura ◽  
Donatella Pastore ◽  
Paolo Bonini ◽  
Paolo Di Nardo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signal Transduction ◽  
Ischemia Reperfusion ◽  
Signal Transduction Pathways ◽  
Stress Model

scholarly journals Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Masoumeh Akhlaghi ◽  
Brian Bandy
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Relative Effectiveness ◽  
Epigallocatechin Gallate ◽  
Short Term ◽  
Higher Doses

While flavonoids can reportedly protect against cardiac ischemia-reperfusion injury, the relative effectiveness of different flavonoids and the mechanisms involved are unclear. We compared protection by different flavonoids using rat embryonic ventricular H9c2 cells subjected to simulated ischemia-reperfusion (IR) and totert-butyl hydroperoxide (t-buOOH). Characterization of the IR model showed the relative contributions of glucose, serum, and oxygen deprivation to cell death. With long-term (2-3 day) pretreatment before IR the best protection was given by catechin, epigallocatechin gallate, proanthocyanidins, and ascorbate, which protected at all doses. Quercetin protected (34%) at 5 μM but was cytotoxic at higher doses. Cyanidin protected mildly (10–15%) at 5 and 20 μM, while delphinidin had no effect at 5 μM and was cytotoxic at higher doses. Comparing long-term and acute protection by catechin, a higher concentration was needed for benefit with acute (1 hr) pretreatment. With a pure oxidative stress (t-buOOH) only quercetin significantly protected with 3-day pretreatment, while with short-term (1 h) pretreatments protection was best with quercetin and epigallocatechin gallate. The results suggest catechins to be especially useful as IR preconditioning agents, while quercetin and epigallocatechin gallate may be the most protective acutely in situations of oxidative stress.

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