Inhibition of gap junction intercellular communication by extremely low-frequency electromagnetic fields in osteoblast-like models is dependent on cell differentiation

2002 ◽  
Vol 190 (2) ◽  
pp. 180-188 ◽  
Author(s):  
Dean T. Yamaguchi ◽  
Jason Huang ◽  
Defang Ma ◽  
Paul K.C. Wang
Keyword(s):  
Cell Differentiation ◽  
Gap Junction ◽  
Electromagnetic Fields ◽  
Intercellular Communication ◽  
Low Frequency ◽  
Extremely Low Frequency ◽  
Gap Junction Intercellular Communication

scholarly journals Extremely Low-Frequency Electromagnetic Fields Affect Myogenic Processes in C2C12 Myoblasts: Role of Gap-Junction-Mediated Intercellular Communication

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Caterina Morabito ◽  
Nathalie Steimberg ◽  
Francesca Rovetta ◽  
Jennifer Boniotti ◽  
Simone Guarnieri ◽  
...  
Keyword(s):  
Gap Junction ◽  
Electromagnetic Fields ◽  
Connexin 43 ◽  
Low Frequency ◽  
Cell Permeability ◽  
C2c12 Myoblast ◽  
Dye Transfer ◽  
C2c12 Myoblasts ◽  
Extremely Low Frequency ◽  
Therapeutic Benefits

Extremely low-frequency electromagnetic fields (ELF-EMFs) can interact with biological systems. Although they are successfully used as therapeutic agents in physiatrics and rehabilitative practice, they might represent environmental pollutants and pose a risk to human health. Due to the lack of evidence of their mechanism of action, the effects of ELF-EMFs on differentiation processes in skeletal muscle were investigated. C2C12 myoblasts were exposed to ELF-EMFs generated by a solenoid. The effects of ELF-EMFs on cell viability and on growth and differentiation rates were studied using colorimetric and vital dye assays, cytomorphology, and molecular analysis of MyoD and myogenin expression, respectively. The establishment of functional gap junctions was investigated analyzing connexin 43 expression levels and measuring cell permeability, using microinjection/dye-transfer assays. The ELF-EMFs did not affect C2C12 myoblast viability or proliferation rate. Conversely, at ELF-EMF intensity in the mT range, the myogenic process was accelerated, through increased expression of MyoD, myogenin, and connexin 43. The increase in gap-junction function suggests promoting cell fusion and myotube differentiation. These data provide the first evidence of the mechanism through which ELF-EMFs may provide therapeutic benefits and can resolve, at least in part, some conditions of muscle dysfunction.

T‐Cell Differentiation to T Helper 9 Phenotype is Elevated by Extremely Low‐Frequency Electromagnetic Fields Via Induction of IL‐2 Signaling

2019 ◽  
Vol 40 (8) ◽  
pp. 588-601
Author(s):  
Ye Won Jang ◽  
Ki Cheol Gil ◽  
Ji Soo Lee ◽  
WonKu Kang ◽  
So‐Young Park ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Electromagnetic Fields ◽  
Low Frequency ◽  
T Cell Differentiation ◽  
T Helper ◽  
Extremely Low Frequency

Connexin expression by alveolar epithelial cells is regulated by extracellular matrix

2001 ◽  
Vol 280 (2) ◽  
pp. L191-L202 ◽  
Author(s):  
Yihe Guo ◽  
Cara Martinez-Williams ◽  
Clare E. Yellowley ◽  
Henry J. Donahue ◽  
D. Eugene Rannels
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Gap Junction ◽  
Intercellular Communication ◽  
Translational Regulation ◽  
Alveolar Epithelial Cells ◽  
Alveolar Type ◽  
Type Ii ◽  
Gap Junction Intercellular Communication ◽  
Type Ii Cell

Extracellular matrix (ECM) proteins promote attachment, spreading, and differentiation of cultured alveolar type II epithelial cells. The present studies address the hypothesis that the ECM also regulates expression and function of gap junction proteins, connexins, in this cell population. Expression of cellular fibronectin and connexin (Cx) 43 increase in parallel during early type II cell culture as Cx26 expression declines. Gap junction intercellular communication is established over the same interval. Cells plated on a preformed, type II cell-derived, fibronectin-rich ECM demonstrate accelerated formation of gap junction plaques and elevated gap junction intercellular communication. These effects are blocked by antibodies against fibronectin, which cause redistribution of Cx43 protein from the plasma membrane to the cytoplasm. Conversely, cells cultured on a laminin-rich ECM, Matrigel, express low levels of Cx43 but high levels of Cx26, reflecting both transcriptional and translational regulation. Cx26 and Cx43 thus demonstrate reciprocal regulation by ECM constituents.

Extremely low-frequency electromagnetic fields activate the antioxidant pathway Nrf2 in a Huntington's disease-like rat model

2013 ◽  
Vol 6 (1) ◽  
pp. 84-86 ◽  
Author(s):  
Inmaculada Tasset ◽  
Aleyda Pérez-Herrera ◽  
Francisco J. Medina ◽  
Óscar Arias-Carrión ◽  
René Drucker-Colín ◽  
...  
Keyword(s):  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Electromagnetic Fields ◽  
Rat Model ◽  
Low Frequency ◽  
Extremely Low Frequency
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