Oxygen-mediated regulation of skeletal muscle satellite cell proliferation and adipogenesis in culture

2001 ◽  
Vol 189 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Marie Csete ◽  
Jean Walikonis ◽  
Nicole Slawny ◽  
Yuewang Wei ◽  
Sheryl Korsnes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Satellite Cell ◽  
Satellite Cell Proliferation ◽  
Skeletal Muscle Satellite Cell

MiR-27b promotes sheep skeletal muscle satellite cell proliferation by targeting myostatin gene

2018 ◽  
Vol 97 (5) ◽  
pp. 1107-1117 ◽  
Author(s):  
Wei Zhang ◽  
Shi-Yin Wang ◽  
Shuang-Yi Deng ◽  
Li Gao ◽  
Li-Wei Yang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Satellite Cell ◽  
Myostatin Gene ◽  
Satellite Cell Proliferation ◽  
Skeletal Muscle Satellite Cell

Multiple signaling pathways mediate LIF-induced skeletal muscle satellite cell proliferation

2002 ◽  
Vol 283 (1) ◽  
pp. C204-C211 ◽  
Author(s):  
Espen E. Spangenburg ◽  
Frank W. Booth
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Growth Factors ◽  
Protein Kinase ◽  
Satellite Cell ◽  
Muscle Satellite Cell ◽  
Signaling Mechanisms ◽  
Stat3 Phosphorylation ◽  
Satellite Cell Proliferation ◽  
Skeletal Muscle Satellite Cell

There are many known growth factors/cytokines that induce skeletal muscle satellite cell proliferation. Currently, the signaling mechanisms in which these growth factors/cytokines activate satellite cell proliferation are not completely understood. Here, we sought to determine signaling mechanisms by which leukemia inhibitory factor (LIF) induces satellite cell proliferation in culture. First, we confirmed that LIF induces proliferation of C2C12 immortalized myoblasts and cultured primary rat satellite cells. In addition, we also found that this increase in proliferation can be inhibited by incubation of the cells in tyrphostin AG 490, a specific inhibitor of Janus-activated kinase (JAK) 2 activity. Furthermore, we also found that incubation of the cells at various time points with LIF (10 ng/ml) induces a significant, transient increase in JAK2 phosphorylation, signal transducers and activators of transcription (STAT3) phosphorylation, and STAT3 transcriptional activity. Increases in the STAT3-sensitive endogenous SOC3 protein followed these transient increases in STAT3 activation. In addition, AG 490 inhibited the increase in STAT3 phosphorylation. Finally, LIF did not change the phosphorylation status of extracellular signal-regulated protein kinase (ERK)1/2 or affect the phosphorylation status of Akt/protein kinase B. However, LY-294002, an inhibitor of phosphoinositide 3-kinase, blocked LIF-induced proliferation of satellite cells. These data suggest that LIF induces satellite cell proliferation by activation of the JAK2-STAT3 signaling pathway, suggesting that this may be an important pathway in muscle growth and/or hypertrophy.

scholarly journals Comparative Analysis of Skeletal Muscle DNA Methylation and Transcriptome of the Chicken Embryo at Different Developmental Stages

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinshan Ran ◽  
Jingjing Li ◽  
Lingqian Yin ◽  
Donghao Zhang ◽  
Chunlin Yu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dna Methylation ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Development ◽  
Muscle Satellite Cell ◽  
Genome Wide ◽  
Embryonic Muscle ◽  
Skeletal Muscle Satellite Cell ◽  
Chicken Skeletal Muscle

DNA methylation is a key epigenetic mechanism involved in embryonic muscle development and plays an important role in early muscle development. In this study, we sought to investigate the effects of genome-wide DNA methylation by combining the expression profiles of the chicken embryonic muscle. Genome-wide DNA methylation maps and transcriptomes of muscle tissues collected from different embryonic development points (E7, E11, E17, and D1) were used for whole-genome bisulfite sequencing (WGBS) and RNA sequencing, respectively. We found that the differentially methylated genes (DMGs) were significantly associated with muscle organ development, regulation of skeletal muscle satellite cell proliferation, and actin filament depolymerization. Furthermore, genes TBX1, MEF2D, SPEG, CFL2, and TWF2 were strongly correlated with the methylation-caused expression switch. Therefore, we chose the CFL2 gene to explore its function in skeletal muscle satellite cells, and the in vitro experiments showed that CFL2 acts as a negative regulator of chicken skeletal muscle satellite cell proliferation and can induce cell apoptosis. These results provide valuable data for future genome and epigenome studies of chicken skeletal muscle and may help reveal the molecular mechanisms of potential economic traits.

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