scholarly journals Effects of PAK4/LIMK1/Cofilin‐1 signaling pathway on proliferation, invasion, and migration of human osteosarcoma cells

2020 ◽  
Vol 34 (9) ◽  
Author(s):  
Zhi‐Feng Li ◽  
Yin‐Di Yao ◽  
Yin‐Yin Zhao ◽  
Yan Liu ◽  
Zhen‐Hua Liu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Osteosarcoma Cells ◽  
Invasion And Migration ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
And Migration

scholarly journals CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells

2017 ◽  
Author(s):  
Zhenhuan Jiang ◽  
Jiannong Jiang ◽  
Bizeng Zhao ◽  
Huilin Yang ◽  
Yunliang Wang ◽  
...  
Keyword(s):  
Osteosarcoma Cells ◽  
Invasion And Migration ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
And Migration

scholarly journals Tomatidine Represses Invasion and Migration of Human Osteosarcoma U2OS and HOS Cells by Suppression of Presenilin 1 and c-Raf–MEK–ERK Pathway

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 326 ◽  
Author(s):  
Min-Hong Hsieh ◽  
Jia-Sin Yang ◽  
Renn-Chia Lin ◽  
Yi-Hsien Hsieh ◽  
Shun-Fa Yang ◽  
...  
Keyword(s):  
Presenilin 1 ◽  
Potential Candidate ◽  
Erk Pathway ◽  
Invasion And Migration ◽  
Cellular Invasion ◽  
Human Osteosarcoma ◽  
Extracellular Signal ◽  
Human Osteosarcoma Cells ◽  
U2os Cells ◽  
And Migration

Osteosarcoma, which is the most prevalent malignant bone tumor, is responsible for the great majority of bone cancer-associated deaths because of its highly metastatic potential. Although tomatidine is suggested to serve as a chemosensitizer in multidrug-resistant tumors, the anti-metastatic effect of tomatidine in osteosarcoma is still unknown. Here, we tested the hypothesis that tomatidine suppresses migration and invasion, features that are associated with metastatic process in human osteosarcoma cells and also investigate its underlying pathway. Tomatidine, up to 100 μM, without cytotoxicity, inhibited the invasion and migration capabilities of human osteosarcoma U2OS and HOS cells and repressed presenilin 1 (PS-1) expression of U2OS cells. After the knockdown of PS-1, U2OS and HOS cells’ biological behaviors of cellular invasion and migratory potential were significantly reduced. While tomatidine significantly decreased the phosphorylation of c-Raf, mitogen/extracellular signal-regulated kinase (MEK), and extracellular signal-regulated protein kinase (ERK)1/2 in U2OS cells, no obvious influences on p-Jun N-terminal kinase, p38, and Akt, including their phosphorylation, were observed. In ERK 1 silencing U2 OS cells, tomatidine further enhanced the decrease of their migratory potential and invasive activities. We conclude that both PS-1 derived from U2OS and HOS cells and the c-Raf–MEK–ERK pathway contribute to cellular invasion and migration and tomatidine could inhibit the phenomenons. These findings indicate that tomatidine might be a potential candidate for anti-metastasis treatment of human osteosarcoma.

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