scholarly journals Dot enzyme-linked immunosorbent assay strip as a screening tool for detection of autoantibody to interferon gamma in sera of suspected cases of adult-onset immunodeficiency

2018 ◽  
Vol 32 (7) ◽  
pp. e22460 ◽  
Author(s):  
Kritsadee Rattanathammethee ◽  
Kriangkrai Chawansuntati ◽  
Romanee Chaiwarith ◽  
Jutarat Praparattanapan ◽  
Khuanchai Supparatpinyo ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Immunosorbent Assay ◽  
Screening Tool ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adult Onset

scholarly journals Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 987
Author(s):  
Weeraya Thongkum ◽  
Umpa Yasamut ◽  
Koollawat Chupradit ◽  
Supachai Sakkhachornphop ◽  
Jiraprapa Wipasa ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Interferon Gamma ◽  
Colloidal Gold ◽  
Enzyme Linked Immunosorbent Assay ◽  
Adult Onset ◽  
Elisa Titer ◽  
Mycobacterial Diseases ◽  
Ifn Γ ◽  
Immunodeficiency Syndrome ◽  
Specific Symptoms

Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was created. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptavidin (6HISMBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.

scholarly journals Evaluation of Standard E TB-Feron Enzyme-Linked Immunosorbent Assay for Diagnosis of Latent Tuberculosis Infection in Health Care Workers

2019 ◽  
Vol 57 (12) ◽  
Author(s):  
Oh Joo Kweon ◽  
Yong Kwan Lim ◽  
Hye Ryoun Kim ◽  
Tae-Hyoung Kim ◽  
Mi-Kyung Lee
Keyword(s):  
Health Care ◽  
Interferon Gamma ◽  
Immunosorbent Assay ◽  
Health Care Workers ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Tube ◽  
Care Workers

ABSTRACT The laboratory diagnosis of latent tuberculosis infection (LTBI) is mainly performed with interferon gamma release assays (IGRAs). We compared the performance of a new enzyme-linked immunosorbent assay (ELISA)-based IGRA, the Standard E TB-Feron ELISA (TBF; SD Biosensor, Gyeonggi-do, Republic of Korea), with that of a widely used assay, the QuantiFERON-TB Gold In-Tube assay (QFT-GIT; Qiagen, Hilden, Germany), in a population of 425 health care workers (HCWs). All HCWs were screened by both assays per the manufacturers’ protocols and in a cross-manner, where tube sets from one assay were used with the alternative ELISA. The results were compared both qualitatively and quantitatively. TBF and QFT-GIT identified 11.3% (48/425) and 12.9% (55/425) of the positive samples, respectively. TBF demonstrated 81.6% positive and 97.4% negative percent agreement with QFT-GIT, with a Cohen’s kappa value of 0.78 (strong agreement). Discordant results were detected in 20 subjects (4.3%): 13 samples (65.0%) were TBF negative and QFT-GIT positive, 6 samples (30.0%) were TBF positive and QFT-GIT negative, and 1 sample provided TBF and QFT-GIT indeterminate/negative results. We observed a statistically significant degree of correlation between the interferon gamma reactivity between the two assays (Spearman’s rho [rs] value = 0.551, P < 0.01) and between standard assays and cross-manner tests (rs value range, 0.449 to 0.816; P < 0.01 for all combinations). Cross-manner tests also revealed that the ELISA kit of TBF provided higher values for the tube containing the tuberculosis (TB) antigen and the negative-control tube than the ELISA of QFT-GIT under the same conditions (P < 0.01), although these differences disappeared when the value for the negative-control tube was subtracted from that for the TB antigen tube. TBF showed a comparable and acceptable clinical performance in detecting LTBI compared to QFT-GIT. TBF represents a useful alternative tool as an ELISA-based IGRA, especially for large-scale screening for LTBI in HCWs.

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