scholarly journals The Diagnostic Value and Performance Evaluation of Five Serological Tests for the Detection ofTreponema pallidum

2014 ◽  
Vol 28 (3) ◽  
pp. 204-209 ◽  
Author(s):  
Can Liu ◽  
Qishui Ou ◽  
Huijuan Chen ◽  
Jing Chen ◽  
Sheng Lin ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Diagnostic Value ◽  
Serological Tests ◽  
And Performance

scholarly journals Detection ofToxoplasma gondiiDNA in horse meat from supermarkets in France and performance evaluation of two serological tests

Parasite ◽  
2015 ◽  
Vol 22 ◽  
pp. 14 ◽  
Author(s):  
Abdelkrim Aroussi ◽  
Philippe Vignoles ◽  
François Dalmay ◽  
Laurence Wimel ◽  
Marie-Laure Dardé ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Serological Tests ◽  
Horse Meat ◽  
And Performance

scholarly journals A Flow Cytometry-Based Serological Assay to Detect Visceral Leishmaniasis in HIV-Infected Patients

2021 ◽  
Vol 8 ◽  
Author(s):  
Elis D. da Silva ◽  
Beatriz C. de Oliveira ◽  
Allana M. de S. Pereira ◽  
Diego L. Guedes ◽  
Osvaldo P. de Melo Neto ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Clinical Status ◽  
Humoral Response ◽  
Diagnostic Value ◽  
Roc Curve Analysis ◽  
Serological Tests ◽  
Negative Results ◽  
And Performance

Visceral Leishmaniasis (VL) is a severe parasitic disease that has emerged as an important opportunistic condition in HIV-infected patients and whose control is impaired by inaccurate identification. This is mainly due to the serological tests used for VL having a reduced performance in cases of VL-HIV coinfection due to a low humoral response. In this situation, however, a positive test has even greater diagnostic value when combined with the clinical status. This study aimed to evaluate the application and performance of flow cytometry to detect anti-Leishmania infantum antibodies in HIV-infected patients. Sera from VL/HIV coinfected patients, characterized using “gold standard” techniques, were compared with sera from healthy controls plus sera from HIV-infected individuals. The flow cytometry results were expressed as levels of IgG reactivity, based on the percentage of positive fluorescent parasites (PPFP). A ROC curve analysis of a serum titration indicated a PPFP of 1.26% as being the cutoff point to segregate positive and negative results. At the 1:2,048 dilution, with 89% sensitivity and 83% specificity, flow cytometry showed greater sensitivity in relation to the serological tests evaluated. Futhermore, flow cytometry was the only assay that positively identified all VL-HIV patients with quantified HIV load. Together, these findings suggest that flow cytometry may be used as an alternative serological approach for VL identification and as a tool to characterize the humoral response against Leishmania infantum in HIV-infected patients.

NUMERICAL INVESTIGATIONS AND PERFORMANCE EVALUATION OF A FOLDED TUBE HEAT TRANSPORT SYSTEM UNDER BOILING CONDITIONS

2018 ◽  
Vol 30 (4) ◽  
pp. 267-291
Author(s):  
Mukesh Kumar ◽  
Avinash Moharana ◽  
Raj K. Singh ◽  
Arun K. Nayak ◽  
Jyeshtharaj B. Joshi
Keyword(s):  
Performance Evaluation ◽  
Heat Transport ◽  
Transport System ◽  
Numerical Investigations ◽  
And Performance
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