scholarly journals High specificity of semi-nested multiplex PCR using dried blood spots on DNA Banking Card in comparison with frozen liquid blood for detection of Plasmodium falciparum and Plasmodium vivax

2011 ◽  
Vol 25 (3) ◽  
pp. 185-190 ◽  
Author(s):  
S. Ataei ◽  
M. Nateghpour ◽  
H. Hajjaran ◽  
G.H. Edrissian ◽  
A. Rahimi Foroushani
Keyword(s):  
Plasmodium Falciparum ◽  
Multiplex Pcr ◽  
Plasmodium Vivax ◽  
High Specificity ◽  
Dried Blood Spots ◽  
Dna Banking ◽  
Blood Spots

scholarly journals Missed Plasmodium falciparum and Plasmodium vivax Mixed Infections in Ethiopia Threaten Malaria Elimination

2021 ◽  
Author(s):  
Colleen M. Leonard ◽  
Hussein Mohammed ◽  
Mekonnen Tadesse ◽  
Jessica N. McCaffery ◽  
Doug Nace ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Lactate Dehydrogenase ◽  
Plasmodium Vivax ◽  
Therapeutic Efficacy ◽  
Dried Blood Spots ◽  
Mixed Infections ◽  
Efficacy Study ◽  
Chain Reaction ◽  
Polymerase Chain

Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia. This study investigated whether mixed infections were missed by microscopy from a 2017 therapeutic efficacy study at two health facilities in Ethiopia. All patients (N = 304) were initially classified as having single-species P. falciparum (n = 148 samples) or P. vivax infections (n = 156). Dried blood spots were tested for Plasmodium antigens by bead-based multiplex assay for pan-Plasmodium aldolase, pan-Plasmodium lactate dehydrogenase, P. vivax lactate dehydrogenase, and histidine-rich protein 2. Of 304 blood samples, 13 (4.3%) contained both P. falciparum and P. vivax antigens and were analyzed by polymerase chain reaction for species-specific DNA. Of these 13 samples, five were confirmed by polymerase chain reaction for P. falciparum/P. vivax co-infection. One sample, initially classified as P. vivax by microscopy, was found to only have Plasmodium ovale DNA. Plasmodium falciparum/P. vivax mixed infections can be missed by microscopy even in the context of a therapeutic efficacy study with multiple trained readers.

scholarly journals DEVELOPMENT OF REAL-TIME MULTIPLEX PCR FOR THE QUANTITATIVE DETERMINATION OF TREC'S AND KREC'S IN WHOLE BLOOD AND IN DRIED BLOOD SPOTS

2015 ◽  
Vol 17 (5) ◽  
pp. 467 ◽  
Author(s):  
M. A. Gordukova ◽  
I. P. Oskorbin ◽  
O. V. Mishukova ◽  
S. B. Zimin ◽  
N. V. Zinovieva ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Multiplex Pcr ◽  
Real Time ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Blood Spots

A single-round multiplex PCR assay for the identification of Anopheles minimus related species infected with Plasmodium falciparum and Plasmodium vivax

2014 ◽  
Vol 63 (2) ◽  
pp. 442-449 ◽  
Author(s):  
Paiboon Eamkum ◽  
Sungsit Sungvornyothin ◽  
Onanong Kritpetcharat ◽  
Jureerat Daduang ◽  
Usa Lek-Uthai ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Multiplex Pcr ◽  
Plasmodium Vivax ◽  
Related Species ◽  
Pcr Assay ◽  
Anopheles Minimus ◽  
Multiplex Pcr Assay

scholarly journals Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Samuel O. Oyola ◽  
Cristina V. Ariani ◽  
William L. Hamilton ◽  
Mihir Kekre ◽  
Lucas N. Amenga-Etego ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome Amplification ◽  
Dried Blood Spots ◽  
Whole Genome ◽  
Genome Amplification ◽  
Blood Spots

scholarly journals Antibody signatures of asymptomatic Plasmodium falciparum malaria infections measured from dried blood spots

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Christine F. Markwalter ◽  
Myat Htut Nyunt ◽  
Zay Yar Han ◽  
Ricardo Henao ◽  
Aarti Jain ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Specific Antibody ◽  
Predictive Value ◽  
Dried Blood Spots ◽  
Protein Microarrays ◽  
Antibody Responses ◽  
Antibody Activity ◽  
Low Density ◽  
Blood Spots ◽  
Diagnostic Characteristics

Abstract Background Screening malaria-specific antibody responses on protein microarrays can help identify immune factors that mediate protection against malaria infection, disease, and transmission, as well as markers of past exposure to both malaria parasites and mosquito vectors. Most malaria protein microarray work has used serum as the sample matrix, requiring prompt laboratory processing and a continuous cold chain, thus limiting applications in remote locations. Dried blood spots (DBS) pose minimal biohazard, do not require immediate laboratory processing, and are stable at room temperature for transport, making them potentially superior alternatives to serum. The goals of this study were to assess the viability of DBS as a source for antibody profiling and to use DBS to identify serological signatures of low-density Plasmodium falciparum infections in malaria-endemic regions of Myanmar. Methods Matched DBS and serum samples from a cross-sectional study in Ingapu Township, Myanmar were probed on protein microarrays populated with P. falciparum antigen fragments. Signal and trends in both sample matrices were compared. A case-control study was then performed using banked DBS samples from malaria-endemic regions of Myanmar, and a regularized logistic regression model was used to identify antibody signatures of ultrasensitive PCR-positive P. falciparum infections. Results Approximately 30% of serum IgG activity was recovered from DBS. Despite this loss of antibody activity, antigen and population trends were well-matched between the two sample matrices. Responses to 18 protein fragments were associated with the odds of asymptomatic P. falciparum infection, albeit with modest diagnostic characteristics (sensitivity 58%, specificity 85%, negative predictive value 88%, and positive predictive value 52%). Conclusions Malaria-specific antibody responses can be reliably detected, quantified, and analysed from DBS, opening the door to serological studies in populations where serum collection, transport, and storage would otherwise be impossible. While test characteristics of antibody signatures were insufficient for individual diagnosis, serological testing may be useful for identifying exposure to asymptomatic, low-density malaria infections, particularly if sero-surveillance strategies target individuals with low previous exposure as sentinels for population exposure.

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