scholarly journals Serodiagnosis ofChlamydia pneumoniaeinfection using three inclusion membrane proteins

2010 ◽  
Vol 24 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Chen Hongliang ◽  
Zhou Zhou ◽  
Hu Zhan ◽  
Zeng Yanhua ◽  
Li Zhongyu ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins

Inclusion membrane proteins of Chlamydiaceae

2008 ◽  
Vol 2 (2) ◽  
pp. 148-159 ◽  
Author(s):  
E. S. Kostryukova ◽  
V. N. Lazarev ◽  
V. M. Govorum
Keyword(s):  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins

scholarly journals Absence of Specific Chlamydia trachomatis Inclusion Membrane Proteins Triggers Premature Inclusion Membrane Lysis and Host Cell Death

Cell Reports ◽  
2017 ◽  
Vol 19 (7) ◽  
pp. 1406-1417 ◽  
Author(s):  
Mary M. Weber ◽  
Jennifer L. Lam ◽  
Cheryl A. Dooley ◽  
Nicholas F. Noriea ◽  
Bryan T. Hansen ◽  
...  
Keyword(s):  
Cell Death ◽  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Host Cell ◽  
Inclusion Membrane ◽  
Host Cell Death ◽  
Membrane Lysis ◽  
Inclusion Membrane Proteins

scholarly journals Characterization of Fifty Putative Inclusion Membrane Proteins Encoded in the Chlamydia trachomatis Genome

2008 ◽  
Vol 76 (6) ◽  
pp. 2746-2757 ◽  
Author(s):  
Zhongyu Li ◽  
Chaoqun Chen ◽  
Ding Chen ◽  
Yimou Wu ◽  
Youmin Zhong ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Chlamydial Infection ◽  
Distribution Patterns ◽  
Western Blot Assay ◽  
Inclusion Membrane ◽  
Urogenital Infection ◽  
Infected Cells ◽  
Inclusion Membrane Proteins

ABSTRACT Although the Chlamydia trachomatis genome is predicted to encode 50 inclusion membrane proteins, only 18 have been experimentally localized in the inclusion membrane of C. trachomatis-infected cells. Using fusion proteins and anti-fusion protein antibodies, we have systematically evaluated all 50 putative inclusion membrane proteins for their localization in the infected cells, distribution patterns, and effects on subsequent chlamydial infection when expressed ectopically, as well as their immunogenicity during chlamydial infection in humans. Twenty-two of the 50 proteins were localized in the inclusion membrane, and 7 were detected inside the inclusions, while the location of the remaining 21 was not defined. Four (CT225, CT228, CT358, and CT440) of the 22 inclusion membrane-localized proteins were visualized in the inclusion membrane of Chlamydia-infected cells for the first time in the current study. The seven intra-inclusion-localized proteins were confirmed to be chlamydial organism proteins in a Western blot assay. Further characterization of the 50 proteins revealed that neither colocalization with host cell endoplasmic reticulum nor inhibition of subsequent chlamydial infection by ectopically expressed proteins correlated with the inclusion membrane localization. Interestingly, antibodies from women with C. trachomatis urogenital infection preferentially recognized proteins localized in the inclusion membrane, and the immunodominant regions were further mapped to the region predicted to be on the cytoplasmic side of the inclusion membrane. These observations suggest that most of the inclusion membrane-localized proteins are both expressed and immunogenic during C. trachomatis infection in humans and that the cytoplasmic exposure may enhance the immunogenicity.

Identification and characterization of a Chlamydia trachomatis early operon encoding four novel inclusion membrane proteins

1999 ◽  
Vol 33 (4) ◽  
pp. 753-765 ◽  
Author(s):  
Marci A. Scidmore-Carlson ◽  
Edward I. Shaw ◽  
Cheryl A. Dooley ◽  
Elizabeth R. Fischer ◽  
Ted Hackstadt
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins ◽  
Identification And Characterization

scholarly journals Inclusion Membrane Proteins of Protochlamydia amoebophila UWE25 Reveal a Conserved Mechanism for Host Cell Interaction among the Chlamydiae

2010 ◽  
Vol 192 (19) ◽  
pp. 5093-5102 ◽  
Author(s):  
Eva Heinz ◽  
Daniel D. Rockey ◽  
Jacqueline Montanaro ◽  
Karin Aistleitner ◽  
Michael Wagner ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Host Cell ◽  
Cell Interaction ◽  
Eukaryotic Cell ◽  
Intracellular Bacteria ◽  
Human Pathogens ◽  
Inclusion Membrane ◽  
A Genome ◽  
Inclusion Membrane Proteins ◽  
Host Cell Interaction

ABSTRACT Chlamydiae are a group of obligate intracellular bacteria comprising several important human pathogens. Inside the eukaryotic cell, chlamydiae remain within a host-derived vesicular compartment, termed the inclusion. They modify the inclusion membrane through insertion of unique proteins, which are involved in interaction with and manipulation of the host cell. Among chlamydiae, inclusion membrane proteins have been exclusively found in members of the family Chlamydiaceae, which predominantly infect mammalian and avian hosts. Here, the presence of inclusion membrane proteins in Protochlamydia amoebophila UWE25, a chlamydial endosymbiont of free-living amoebae, is reported. A genome-wide screening for secondary structure motifs resulted in the identification of 23 putative inclusion membrane proteins for this organism. Immunofluorescence analysis demonstrated that five of these proteins were expressed, and four of them could be localized to a halo surrounding the intracellular bacteria. Colocalization studies showed an almost complete overlap of the signals obtained for the four putative inclusion membrane proteins, and immuno-transmission electron microscopy unambiguously demonstrated their location in the inclusion membrane. The presence of inclusion membrane proteins (designated IncA, IncQ, IncR, and IncS) in P. amoebophila shows that this strategy for host cell interaction is conserved among the chlamydiae and is used by chlamydial symbionts and pathogens alike.

scholarly journals Microscopy Techniques Used to Visualize Immune-Labeled Chlamydial Inclusion Membrane Proteins in 2D and 3D

2019 ◽  
Vol 25 (S2) ◽  
pp. 1166-1167
Author(s):  
Benjamin Crews ◽  
Elizabeth R. Fischer ◽  
Forrest Hoyt ◽  
Bryan T. Hansen ◽  
Ted Hackstadt
Keyword(s):  
Membrane Proteins ◽  
Inclusion Membrane ◽  
Inclusion Membrane Proteins ◽  
Microscopy Techniques ◽  
2D And 3D ◽  
Chlamydial Inclusion
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