scholarly journals Magnesium and calcium in exhaled breath condensate of children with asthma and gastroesophageal reflux disease

2009 ◽  
Vol 23 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Slavica Dodig ◽  
Željka Vlašić ◽  
Ivana Čepelak ◽  
Renata Zrinski Topić ◽  
Mirjana Turkalj ◽  
...  
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Children With Asthma ◽  
Breath Condensate

scholarly journals Study of Various Parameters that Influence the Content of Exhaled Breath Condensate Used in the Diagnosis of Gastroesophageal Reflux Disease

2018 ◽  
Vol 46 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Michal Greguš ◽  
Pavlo Ďurč ◽  
Julia Lačná ◽  
František Foreti ◽  
Peter Kubáň
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Exhaled Breath Condensate ◽  
Background Electrolyte ◽  
Exhaled Breath ◽  
Sampling Device ◽  
Rapid Separation ◽  
Ionic Content ◽  
Breath Condensate

Abstract In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in of the noninvasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 M of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability

Su1073 - Assessment of Exhaled Breath Condensate for Non-Invasive Diagnosis of Gastroesophageal Reflux Disease

2018 ◽  
Vol 154 (6) ◽  
pp. S-477
Author(s):  
Stefan Konecny ◽  
Jiri Dolina ◽  
Martina Doubkova ◽  
Jana Skrickova ◽  
Dagmar Kindlova ◽  
...  
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Non Invasive ◽  
Breath Condensate

scholarly journals Capillary Electrophoretic Analysis of Exhaled Breath Condensate in the Diagnosis of Gastroesophageal Reflux Disease

2018 ◽  
Vol 46 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Peter Kubáň ◽  
Pavol Ďurč ◽  
Julia Lačná ◽  
Michal Greguš ◽  
František Foret ◽  
...  
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Exhaled Breath Condensate ◽  
Electrophoretic Analysis ◽  
Exhaled Breath ◽  
Sample Collection ◽  
Conductometric Detection ◽  
Ethanesulfonic Acid ◽  
Breath Condensate

Abstract In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease

scholarly journals Reflux Revisited: Advancing the Role of Pepsin

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Karna Dev Bardhan ◽  
Vicki Strugala ◽  
Peter W. Dettmar
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Proton Pump ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Extraesophageal Reflux ◽  
Pepsinogen Secretion ◽  
Novel Drugs ◽  
Breath Condensate

Gastroesophageal reflux disease is mediated principally by acid. Today, we recognise reflux reaches beyond the esophagus, where pepsin, not acid, causes damage. Extraesophageal reflux occurs both as liquid and probably aerosol, the latter with a further reach. Pepsin is stable up to pH 7 and regains activity after reacidification. The enzyme adheres to laryngeal cells, depletes its defences, and causes further damage internally after its endocytosis. Extraesophageal reflux can today be detected by recognising pharyngeal acidification using a miniaturised pH probe and by the identification of pepsin in saliva and in exhaled breath condensate by a rapid, sensitive, and specific immunoassay. Proton pump inhibitors do not help the majority with extraesophageal reflux but specifically formulated alginates, which sieve pepsin, give benefit. These new insights may lead to the development of novel drugs that dramatically reduce pepsinogen secretion, block the effects of adherent pepsin, and give corresponding clinical benefit.

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