scholarly journals Simultaneously multi-parameter determination of hematonosis cell apoptosis by two-photon and confocal laser scanning microscopy

2004 ◽  
Vol 18 (5) ◽  
pp. 271-275 ◽  
Author(s):  
Yi Wang ◽  
Xue-Feng Wang ◽  
Chen Wang ◽  
Hui Ma
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Cell Apoptosis ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Parameter Determination ◽  
Confocal Laser ◽  
Two Photon

scholarly journals Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae

2010 ◽  
Vol 16 (S2) ◽  
pp. 1142-1143
Author(s):  
Z Burdíková ◽  
M Čapek ◽  
P Ostasou ◽  
EAD Mitchell ◽  
J Machač ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Testate Amoebae ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

A Simple and Rapid Staining Technique for Sex Determination of Trichinella Larvae Parasites by Confocal Laser Scanning Microscopy

2019 ◽  
Vol 25 (6) ◽  
pp. 1491-1497 ◽  
Author(s):  
Inese Gavarane ◽  
Elena Kirilova ◽  
Ilze Rubeniņa ◽  
Ligita Mežaraupe ◽  
Sergejs Osipovs ◽  
...  
Keyword(s):  
Sex Determination ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Staining Technique ◽  
Life Cycle Stage ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

AbstractThe roundworms of Trichinella genus are worldwide distributed and their prevalence in nature is high. Trichinella genus parasites are the causative agents of foodborne zoonosis trichinellosis. The main prevention and control of the infection are meat inspection by the magnetic stirrer method for the detection of Trichinella larvae in muscle samples. The treatment can be effective if the parasite is discovered early in the intestinal phase. Once the Trichinella larva has reached the muscle tissue, the parasite remains therein and there is no treatment for this life cycle stage. The Trichinella species is dioecious with separate male and female individuals. The developed staining technique that uses confocal laser scanning microscopy (CLSM) displays sufficient results for Trichinella larvae examination and this protocol is applicable to study the internal and external structures and for the sex determination of T. britovi and T. spiralis larvae samples. In the present study, a luminescent derivative was synthesized and used for staining of T. spiralis and T. britovi larvae samples for the examination by CLSM. Various fixatives, such as AFA, 70% ethanol, and Bouin's and Carnoy's solutions were tested for sample preparation. The synthesized luminescent compound demonstrates best visualization results for samples fixed in Bouin's fixative.

scholarly journals QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY

2011 ◽  
Vol 25 (3) ◽  
pp. 111 ◽  
Author(s):  
Merete Krog Raarup ◽  
Jens Randel Nyengaard
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
3D Structure ◽  
Correlation Spectroscopy ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Two Photon ◽  
Photon Excitation

This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation. The use of CLSM for improved stereological length estimation in thick (up to 0.5 mm) tissue is proposed. The techniques of FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), FCS (Fluorescence Correlation Spectroscopy) and FRAP (Fluorescence Recovery After Photobleaching) are introduced and their applicability for quantitative imaging of biomolecular (co-)localization and trafficking in live cells described. The advantage of two-photon versus one-photon excitation in relation to these techniques is discussed.

scholarly journals Rapid Determination of the Distribution of Cellulose Nanomaterial Aggregates in Composites Enabled by Multi-Channel Spectral Confocal Microscopy

2019 ◽  
Vol 25 (3) ◽  
pp. 682-689 ◽  
Author(s):  
Marcus A. Johns ◽  
Anna E. Lewandowska ◽  
Stephen J. Eichhorn
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Rapid Determination ◽  
Aggregate Size ◽  
Raman Imaging ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

AbstractThere is increased interest in the use of cellulose nanomaterials for the mechanical reinforcement of composites due to their high stiffness and strength. However, challenges remain in accurately determining their distribution within composite microstructures. We report the use of a range of techniques used to image aggregates of cellulose nanocrystals (CNCs) greater than 10 µm2 within a model thermoplastic polymer. While Raman imaging accurately determines CNC aggregate size, it requires extended periods of analysis and the limited observable area results in poor reproducibility. In contrast, staining the CNCs with a fluorophore enables rapid acquisition with high reproducibility, but overestimates the aggregate size as CNC content increases. Multi-channel spectral confocal laser scanning microscopy is presented as an alternative technique that combines the accuracy of Raman imaging with the speed and reproducibility of conventional confocal laser scanning microscopy, enabling the rapid determination of CNC aggregate distribution within composites.

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