SCF FBXW17 E3 ubiquitin ligase regulates FBXL19 stability and cell migration

2020 ◽  
Author(s):  
Su Dong ◽  
Jianxin Wei ◽  
Rachel K. Bowser ◽  
Bill B. Chen ◽  
Rama K. Mallampalli ◽  
...  
Keyword(s):  
Cell Migration ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase

scholarly journals MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1153
Author(s):  
Aiqin Sun ◽  
Jun Zhu ◽  
Song Xia ◽  
Yanling Li ◽  
Tiantian Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Cancer Cell Migration ◽  
Lung Cancer Cell ◽  
Cancer Migration ◽  
Ubiquitination Assay

Our previous studies have shown that the HECT E3 ubiquitin ligase NEDD4 and kinase MEKK5 both play an essential role in lung cancer migration. A report predicts that MEKK5 may be ubiquitinated by NEDD4; however, interaction of MEKK5 with NEDD4 and ubiquitination of MEKK5 by NEDD4 have not been characterized. In this report, we show that NEDD4 interacts with MEKK5 through a conserved WW3 domain by the co-immunoprecipitation and the GST-pulldown assays. The ubiquitination assay indicates that MEKK5 is not a ubiquitination substrate of NEDD4, but negatively regulates NEDD4-mediated ubiquitination. Furthermore, overexpression of MEKK5 significantly reduced the NEDD4-promoted lung cancer cell migration. Taken together, our studies have defined an inhibitory role of MEKK5 in regulation of NEDD4-mediated ubiquitination.

scholarly journals Cortical dynamics during cell motility are regulated by CRL3KLHL21 E3 ubiquitin ligase

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Thibault Courtheoux ◽  
Radoslav I. Enchev ◽  
Fabienne Lampert ◽  
Juan Gerez ◽  
Jochen Beck ◽  
...  
Keyword(s):  
Cell Migration ◽  
Ubiquitin Ligase ◽  
Cortical Plasticity ◽  
Cortical Microtubule ◽  
E3 Ubiquitin Ligase ◽  
Cell Movement ◽  
Focal Adhesions ◽  
Leading Edge ◽  
Cell Cortex ◽  
Cortical Dynamics

Abstract Directed cell movement involves spatial and temporal regulation of the cortical microtubule (Mt) and actin networks to allow focal adhesions (FAs) to assemble at the cell front and disassemble at the rear. Mts are known to associate with FAs, but the mechanisms coordinating their dynamic interactions remain unknown. Here we show that the CRL3KLHL21 E3 ubiquitin ligase promotes cell migration by controlling Mt and FA dynamics at the cell cortex. Indeed, KLHL21 localizes to FA structures preferentially at the leading edge, and in complex with Cul3, ubiquitylates EB1 within its microtubule-interacting CH-domain. Cells lacking CRL3KLHL21 activity or expressing a non-ubiquitylatable EB1 mutant protein are unable to migrate and exhibit strong defects in FA dynamics, lamellipodia formation and cortical plasticity. Our study thus reveals an important mechanism to regulate cortical dynamics during cell migration that involves ubiquitylation of EB1 at focal adhesions.

scholarly journals Regulation of E3 ubiquitin ligase-1 (WWP1) by microRNA-452 inhibits cancer cell migration and invasion in prostate cancer

2016 ◽  
Vol 114 (10) ◽  
pp. 1135-1144 ◽  
Author(s):  
Yusuke Goto ◽  
Satoko Kojima ◽  
Akira Kurozumi ◽  
Mayuko Kato ◽  
Atsushi Okato ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Migration And Invasion ◽  
Cancer Cell Migration ◽  
Cell Migration And Invasion

scholarly journals The E3 ubiquitin ligase NEDD4 mediates cell migration signaling of EGFR in lung cancer cells

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Genbao Shao ◽  
Ranran Wang ◽  
Aiqin Sun ◽  
Jing Wei ◽  
Ke Peng ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Migration ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Lung Cancer Cells

Author response for "TAM receptors attenuate murine NK cell responses via E3 ubiquitin ligase Cbl‐b"

2019 ◽  
Author(s):  
Leilani M. Chirino ◽  
Suresh Kumar ◽  
Mariko Okumura ◽  
David E. Sterner ◽  
Michael Mattern ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Nk Cell ◽  
E3 Ubiquitin Ligase ◽  
Author Response ◽  
Cell Responses ◽  
Tam Receptors
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