Protective effect of gallic acid on apoptosis of sperm and in vitro fertilization in adult male mice treated with cyclophosphamide

2019 ◽  
Vol 120 (10) ◽  
pp. 17250-17257 ◽  
Author(s):  
Zahra Mehraban ◽  
Marefat Ghaffari Novin ◽  
Mohammad Ghasem Golmohammadi ◽  
Mohsen Sagha ◽  
Khashayar Pouriran ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Protective Effect ◽  
Gallic Acid ◽  
Adult Male ◽  
Male Mice ◽  
Vitro Fertilization

Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex‐sorted bull sperm by inhibiting the oxidative stress

2020 ◽  
Vol 55 (9) ◽  
pp. 1103-1114
Author(s):  
Pei‐Pei Zhang ◽  
Jing‐Jing Wang ◽  
Chong‐Yang Li ◽  
Hai‐sheng Hao ◽  
Hao‐Yu Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Vitamin C ◽  
Protective Effect ◽  
Vitro Fertilization ◽  
Bull Sperm ◽  
Fertilization Capacity

Protective effect of crocetin on bovine spermatozoa against oxidative stress during in vitro fertilization

Andrology ◽  
2016 ◽  
Vol 4 (6) ◽  
pp. 1138-1149 ◽  
Author(s):  
V. Sapanidou ◽  
I. Taitzoglou ◽  
I. Tsakmakidis ◽  
I. Kourtzelis ◽  
D. Fletouris ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Protective Effect ◽  
Vitro Fertilization ◽  
Bovine Spermatozoa

Altered helper T cell-mediated immune responses in male mice conceived through in vitro fertilization

2017 ◽  
Vol 69 ◽  
pp. 196-203 ◽  
Author(s):  
Hiwa Karimi ◽  
Pooya Mahdavi ◽  
Shohreh Fakhari ◽  
Mohammad Reza Faryabi ◽  
Parisa Esmaeili ◽  
...  
Keyword(s):  
T Cell ◽  
In Vitro Fertilization ◽  
Immune Responses ◽  
Male Mice ◽  
Helper T Cell ◽  
Vitro Fertilization

Mouse in vitro fertilization and culture and a suboptimal diet are associated with decreased anxiety in adult male offspring

2007 ◽  
Vol 88 ◽  
pp. S128
Author(s):  
P.F. Rinaudo ◽  
A. Donjacour ◽  
L. Delle Piane ◽  
F. Di Sebastiano ◽  
A. Dobson ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Adult Male ◽  
Male Offspring ◽  
Vitro Fertilization ◽  
Mouse In Vitro Fertilization

Testosterone Supplementation Rescues Spermatogenesis and In Vitro Fertilizing Ability of Sperm in Kiss1 Knockout Mice

Endocrinology ◽  
2020 ◽  
Vol 161 (9) ◽  
Author(s):  
Teppei Goto ◽  
Masumi Hirabayashi ◽  
Youki Watanabe ◽  
Makoto Sanbo ◽  
Koichi Tomita ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Hypogonadotropic Hypogonadism ◽  
Preimplantation Development ◽  
Hormone Deficiency ◽  
Testicular Descent ◽  
Male Mice ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Suitable Animal Model

Abstract Restoration of spermatogenesis and fertility is a major issue to be solved in male mammals with hypogonadotropic hypogonadism. Kiss1 knockout (KO) male mice are postulated to be a suitable animal model to investigate if hormonal replacement rescues spermatogenesis in mammals with this severe reproductive hormone deficiency, because KO mice replicate the hypothalamic disorder causing hypogonadism. The present study investigated whether testosterone supplementation was able to restore spermatogenesis and in vitro fertilization ability in Kiss1 KO mice. To this end, spermatogenesis, in vitro fertilization ability of Kiss1 KO sperm, and preimplantation development of wild-type embryos inseminated with Kiss1 KO sperm, were examined. The newly generated Kiss1 KO male mice showed infertility with cryptorchidism. Subcutaneous testosterone supplementation for 6 weeks restored plasma and intratesticular testosterone levels, elicited testicular descent, and induced complete spermatogenesis from spermatocytes to elongated spermatids in the testis, resulting in an increase in epididymal sperm number in testosterone-supplemented Kiss1 KO male mice. Epididymal sperm derived from the testosterone-supplemented Kiss1 KO mice showed normal in vitro fertilization ability, and the fertilized eggs showed normal preimplantation development, while the males failed to impregnate females. These results suggest that the failure of spermatogenesis in Kiss1 KO mice is mainly due to a lack of testosterone production, and that Kiss1 KO sperm are capable of fertilizing eggs if the animals receive the appropriate testosterone supplementation without local kisspeptin signaling in the testis and epididymis. Thus, testosterone supplementation would restore spermatogenesis of male mammals showing hypogonadotropic hypogonadism with genetic inactivation of the KISS1/Kiss1 gene.

Effect of chronic THC administration in the reproductive organs of male mice, spermatozoa and in vitro fertilization

2018 ◽  
Vol 157 ◽  
pp. 294-303 ◽  
Author(s):  
A.P. López-Cardona ◽  
I. Ibarra-Lecue ◽  
R. Laguna-Barraza ◽  
S. Pérez-Cerezales ◽  
L. Urigüen ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Reproductive Organs ◽  
Male Mice ◽  
Vitro Fertilization

scholarly journals The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother–Embryo Interaction

2021 ◽  
Vol 22 (19) ◽  
pp. 10650
Author(s):  
Galina Vladimirovna Kontsevaya ◽  
Ludmila Alekseevna Gerlinskaya ◽  
Yury Mikhailovich Moshkin ◽  
Margarita Vladimirovna Anisimova ◽  
Aliya Konstantinovna Stanova ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Seminal Fluid ◽  
Negative Impact ◽  
Vaccination Campaign ◽  
Keyhole Limpet Hemocyanin ◽  
Male Mice ◽  
Surrogate Mother ◽  
Surrogate Mothers ◽  
Vitro Fertilization

The latest vaccination campaign has actualized the potential impact of antigenic stimuli on reproductive functions. To address this, we mimicked vaccination’s effects by administering keyhole limpet hemocyanin (KLH ) to CD1 male mice and used their sperm for in vitro fertilization (IVF). Two-cell embryos after IVF with spermatozoa from control (C) or KLH-treated (Im) male mice were transferred to surrogate mothers mated with vasectomized control (C) or KLH-treated (Im) male mice, resulting in four experimental groups: C–C, Im–C, C–Im, and Im–Im. The pre-implantation losses were significantly lower in the Im–C group than in the C–Im group. At the same time, the resorption rates reduced markedly in the C–Im compared to the Im–C group. Embryo and placenta weights were significantly higher in the Im–Im group. Although the GM-CSF levels were lower in the amniotic fluid of the gestating surrogate mothers in the Im–Im group, they were strongly correlated with embryo mass. The number–size trade-off was only significant in the Im–Im group. This suggests a positive, cooperative effect of spermatozoa and seminal fluid from immune-primed males on embryo growth and the optimal distribution of surrogate mother maternal resources despite the negative impact of males’ antigenic challenge on the IVF success rate.

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