Total flavonoids from the Carya cathayensis Sarg. leaves inhibit HUVEC senescence through the miR‐34a/SIRT1 pathway

2019 ◽  
Vol 120 (10) ◽  
pp. 17240-17249 ◽  
Author(s):  
Yan Guo ◽  
Liwan Xing ◽  
Nipi Chen ◽  
Chengxian Gao ◽  
Zhishan Ding ◽  
...  
Keyword(s):  
Total Flavonoids ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg

scholarly journals Involvement of Flavonoids from the Leaves of Carya cathayensis Sarg. in Sirtuin 1 Expression in HUVEC Senescence

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Yan Guo ◽  
Liwan Xing ◽  
Chaodong Qian ◽  
Zhishan Ding ◽  
Bo Jin
Keyword(s):  
Endothelial Cell ◽  
Endothelial Dysfunction ◽  
Molecular Mechanisms ◽  
Umbilical Vein ◽  
Cell Senescence ◽  
Sirtuin 1 ◽  
Total Flavonoids ◽  
The World ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg

Atherosclerosis is the commonest cause of death in the world and one of the most important processes that occurs with increasing age because it is accompanied by progressive endothelial dysfunction. Recent studies demonstrated that Sirtuin 1 (SIRT1) might potentially affect cell senescence. However, the effect of SIRT1 on the regulation of human umbilical vein endothelial cell (HUVEC) senescence with total flavonoids (TFs) has not been addressed previously. This study investigated how SIRT1 functions in the process of HUVEC senescence when TFs are present and identified the potential molecular mechanisms involved. Using a model of HUVEC senescence induced by angiotensin II, TFs pretreatment reduced the percentage of senescence-associated β-galactosidase (SA-β-gal) cells and p53 mRNA expression. The level of SIRT1 protein and E2F1 decreased during HUVEC senescence and could be partially recovered when cells were coincubated with TFs, while the levels of proteins p53 and p21 increased during cell senescence and diminished in response to the TFs treatment. When coincubated with 20 mM nicotinamide, the results with SA-β-gal-positive cells and the expression of SIRT1, E2F1, p53, and p21 were contrary to that obtained with only TFs pretreatment. The data indicate that the TFs exert their effect on HUVEC senescence through SIRT1.

scholarly journals Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Ruibin Jiang ◽  
Yan Guo ◽  
Nipi Chen ◽  
Chengxian Gao ◽  
Zhishan Ding ◽  
...  
Keyword(s):  
Akt Signaling ◽  
Total Flavonoids ◽  
Protective Effects ◽  
H9c2 Cells ◽  
H9c2 Cardiomyocytes ◽  
Reoxygenation Injury ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg ◽  
Signaling Activity ◽  
Hypoxia Reoxygenation

This study aimed to investigate whether the total flavonoids (TFs) from Carya cathayensis Sarg. leaves alleviate hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes and to explore potential mechanisms. H9c2 cells pretreated with TFs for 24h were exposed to H/R treatment. The results indicated that TFs significantly alleviate H/R injury, which include inhibiting apoptosis and enhancing antioxidant capacity. The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. Conversely, blocking miR-21 expression with miR-21 inhibitor effectively suppressed the protective effects of TFs against H/R-induced injury. Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax.

Generation and characterization of nanobubbles in ionic liquid for a green extraction of polyphenols from Carya cathayensis Sarg

2021 ◽  
pp. 130932
Author(s):  
Fatima-ezzahra Ettoumi ◽  
Ruyuan Zhang ◽  
Tarun Belwal ◽  
Miral Javed ◽  
Yanqun Xu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Green Extraction ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg

Molecular docking simulation and in vitro studies on estrogenic activities of flavonoids from leaves of Carya cathayensis Sarg

Steroids ◽  
2020 ◽  
Vol 163 ◽  
pp. 108726
Author(s):  
Jing-Jing Lu ◽  
Fang-Mei Zhou ◽  
Xu-Jiao Hu ◽  
Jing-Jing Fang ◽  
Cai-Xia Liu ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Docking Simulation ◽  
In Vitro Studies ◽  
Molecular Docking Simulation ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg

Effects of intercropping grasses on soil organic carbon and microbial community functional diversity under Chinese hickory (Carya cathayensis Sarg.) stands

Soil Research ◽  
2014 ◽  
Vol 52 (6) ◽  
pp. 575 ◽  
Author(s):  
Jiasen Wu ◽  
Haiping Lin ◽  
Cifu Meng ◽  
Penkun Jiang ◽  
Weijun Fu
Keyword(s):  
Microbial Community ◽  
Microbial Biomass ◽  
Organic Carbon ◽  
Soil Organic Carbon ◽  
Functional Diversity ◽  
Water Soluble ◽  
Organic N ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg ◽  
Chinese Milk Vetch

Chinese hickory (Carya cathayensis Sarg.) is a woody nut and oil tree from China. Intensive management including heavy application of chemical fertiliser and long-term application of herbicides has resulted in serious soil loss and degradation. This study aimed to test the hypothesis that intercropping in the soil under Chinese hickory stands may improve soil fertility and microbial community functional diversity. A field experiment consisting of four treatments (clean tillage; intercropping rape (Brassica rapa L.), ryegrass (Lolium perenne L.) or Chinese milk vetch (Astragalus sinicus L.) was conducted to study the effects of intercropping on soil organic carbon (SOC) structure and microbial community functional diversity under C. cathayensis stand, by means of 13C-nuclear magnetic resonance (NMR), and EcoPlates incubated at 25°C. After 4 years of treatment, intercropping increased available nitrogen (N), phosphorus and potassium in the soil by 25.1–54.2, 4.2–6.0 and 0–22.5 mg kg–1, respectively, relative to the clean tillage treatment; intercropping rape, ryegrass and Chinese milk vetch increased SOC, microbial biomass C (MBC), and water-soluble organic C (WOC) by 23.1–24.7, 138.6–159.7 and 56.2–69.5% (P < 0.05), respectively. The structure of SOC was also greatly changed by intercropping treatments. Intercropping increased carbonyl C by 29.9–36.9% (P < 0.05) and decreased alkyl C, O-alkyl C and aromatic C by 10.0–16.4, 18.9–20.9 and 10.5–16.6% (P < 0.05), respectively. Intercropping markedly improved microbial community functional diversity, which is characterised by increases in average well-colour development (AWCD), Shannon index and evenness index. Correlation analysis showed significant positive correlations among microbial biomass N, water-soluble organic N, SOC, WOC, MBC and AWCD (P < 0.05 or P < 0.01). The results demonstrate that sod cultivation is an effective soil management practice that improves soil quality and eliminates detrimental effects of clean tillage in Chinese hickory production.

Molecular characterization and expression analysis of the critical floral genes in hickory (Carya cathayensis Sarg.)

2014 ◽  
Vol 83 ◽  
pp. 142-150 ◽  
Author(s):  
Chen Shen ◽  
Yingwu Xu ◽  
Jianqin Huang ◽  
Zhengjia Wang ◽  
Jiani Qiu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Expression Analysis ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg

scholarly journals Use of transcriptome sequencing to understand the pistillate flowering in hickory (Carya cathayensis Sarg.)

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 691 ◽  
Author(s):  
You-Jun Huang ◽  
Li-Li Liu ◽  
Jian-Qin Huang ◽  
Zheng-Jia Wang ◽  
Fang-Fang Chen ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg

Characterization of Protein Isolates from Defatted Chinese Hickory (Carya Cathayensis Sarg) Kernels

2011 ◽  
Vol 343-344 ◽  
pp. 368-373
Author(s):  
Zhi Ping He ◽  
Lin Chun Mao ◽  
Cun Shan Zhou ◽  
Feng Hua Wu
Keyword(s):  
Dry Weight ◽  
Extraction Process ◽  
Protein Isolate ◽  
Protein Nitrogen ◽  
Gelation Concentration ◽  
Carya Cathayensis ◽  
Ph Range ◽  
Emulsifying Capacity ◽  
Carya Cathayensis Sarg

Chinese hickory (Carya cathayensis Sarg.) kernel protein isolate (CKPI) was isolated from hinese hickory kernel defatted flour (CKDF) by isoelectric precipitation. CKPI was evaluated for chemical composition and selected functional properties. CKPI contained over 72.0% dry weight (DW) of protein. Nitrogen solubility curves for CKPI were pH-dependent in the pH range of 2.0-12.0 with minimum solubility observed at pH 4.0 (9.8%) and maximum solubility at pH 12.0 (89.9%). Minimum emulsifying capacity (EC) and emulsifying stability (ES) of CKPI were observed at pH 4.0. CKPI had a least gelation concentration of (LGC) of 6% (w/v) at pH 4.0. Results indicated that Chinese hickory kernel may be a new protein source with huge exploitation potential after oil extraction process in China.

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