Role of heterogeneous nuclear ribonucleoprotein K in tumor development

2019 ◽  
Vol 120 (9) ◽  
pp. 14296-14305 ◽  
Author(s):  
Yuting Chen ◽  
Ying Zeng ◽  
Zheng Xiao ◽  
Shi Chen ◽  
Yukun Li ◽  
...  
Keyword(s):  
Tumor Development ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein

scholarly journals Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation

2014 ◽  
Vol 42 (15) ◽  
pp. 9908-9924 ◽  
Author(s):  
Jen-Hao Yang ◽  
Yi-Ying Chiou ◽  
Shu-Ling Fu ◽  
I-Yun Shih ◽  
Tsai-Hsuan Weng ◽  
...  
Keyword(s):  
Dna Damage ◽  
Rna Processing ◽  
Tumor Development ◽  
Arginine Methylation ◽  
Wild Type ◽  
Post Translational Modifications ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Damage Response ◽  
Nuclear Ribonucleoprotein

Abstract Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is an RNA/DNA-binding protein involved in chromatin remodeling, RNA processing and the DNA damage response. In addition, increased hnRNPK expression has been associated with tumor development and progression. A variety of post-translational modifications of hnRNPK have been identified and shown to regulate hnRNPK function, including phosphorylation, ubiquitination, sumoylation and methylation. However, the functional significance of hnRNPK arginine methylation remains unclear. In the present study, we demonstrated that the methylation of two essential arginines, Arg296 and Arg299, on hnRNPK inhibited a nearby Ser302 phosphorylation that was mediated through the pro-apoptotic kinase PKCδ. Notably, the engineered U2OS cells carrying an Arg296/Arg299 methylation-defective hnRNPK mutant exhibited increased apoptosis upon DNA damage. While such elevated apoptosis can be diminished through addition with wild-type hnRNPK, we further demonstrated that this increased apoptosis occurred through both intrinsic and extrinsic pathways and was p53 independent, at least in part. Here, we provide the first evidence that the arginine methylation of hnRNPK negatively regulates cell apoptosis through PKCδ-mediated signaling during DNA damage, which is essential for the anti-apoptotic role of hnRNPK in apoptosis and the evasion of apoptosis in cancer cells.

Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer

2015 ◽  
Vol 11 (3) ◽  
pp. 743-752 ◽  
Author(s):  
Paul Dowling ◽  
Damian Pollard ◽  
AnneMarie Larkin ◽  
Michael Henry ◽  
Paula Meleady ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumour Tissue ◽  
Blood Samples ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein

Role of hnRNPA2B1 in lung cancer.

scholarly journals Role of human heterogeneous nuclear ribonucleoprotein C1/C2 in dengue virus replication

2015 ◽  
Vol 12 (1) ◽  
pp. 14 ◽  
Author(s):  
Thanyaporn Dechtawewat ◽  
Pucharee Songprakhon ◽  
Thawornchai Limjindaporn ◽  
Chunya Puttikhunt ◽  
Watchara Kasinrerk ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein

microR-505/heterogeneous nuclear ribonucleoprotein M inhibits hepatocellular carcinoma cell proliferation and induces cell apoptosis through the Wnt/β-catenin signaling pathway

2020 ◽  
Vol 14 (11) ◽  
pp. 981-996
Author(s):  
Xiaobin Chi ◽  
Yi Jiang ◽  
Yongbiao Chen ◽  
Lizhi Lv ◽  
Jianwei Chen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Apoptosis Assay ◽  
Proliferation And Metastasis ◽  
Novel Strategy ◽  
Nuclear Ribonucleoprotein

Aim: This study aimed to investigate the expression of microRNA-505 (miR-505) and explore its clinical significance, biological function and mechanisms in hepatocellular carcinoma (HCC). Methods: Expression of miR-505 was measured in 128 paired HCC tissues and five cell lines by quantitative real-time polymerase chain reaction (qRT-PCR). MTT assay, Transwell migration, invasion assays and apoptosis assay were performed to explore the functional role of miR-505. The target gene of miR-505 was assessed using the bioinformatics assay and the related signaling pathway was confirmed using western blot. Results: Expression of miR-505 in HCC serum and tissues were downregulated. The overexpression of miR-505 in HCC cells inhibited cell proliferation and metastasis, as well as enhanced cell apoptosis by directly downregulating heterogeneous nuclear ribonucleoprotein M ( HNRNPM). The activity of the Wnt/β-catenin signaling pathway was suppressed by the overexpression of miR-505 but was promoted by the upregulation of HNRNPM. Conclusion: The results suggest that the regulation of miR-505/ HNRNPM may be a novel strategy to improve the targeted therapy of HCC.

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