Mesenchymal stem cells differentiate into keratinocytes and express epidermal kallikreins: Towards an in vitro model of human epidermis

2019 ◽  
Vol 120 (8) ◽  
pp. 13141-13155 ◽  
Author(s):  
Jeniffer Farias dos Santos ◽  
Nathália Ruder Borçari ◽  
Mariana da Silva Araújo ◽  
Viviane Abreu Nunes
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Human Epidermis ◽  
Vitro Model

A scaffold-free in vitro model for osteogenesis of human mesenchymal stem cells

2011 ◽  
Vol 43 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Cornelia Hildebrandt ◽  
Heiko Büth ◽  
Hagen Thielecke
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Human Mesenchymal Stem Cells ◽  
Vitro Model

scholarly journals Anaerobic Co-Culture of Mesenchymal Stem Cells and Anaerobic Pathogens - A New In Vitro Model System

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78226 ◽  
Author(s):  
Katja Kriebel ◽  
Anne Biedermann ◽  
Bernd Kreikemeyer ◽  
Hermann Lang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Model System ◽  
In Vitro Model System ◽  
Vitro Model

scholarly journals Effect of mesenchymal stem cells combined with chondroitin sulfate in an osteoarthritis in vitro model

2020 ◽  
Author(s):  
Saúl Pérez-Castrillo ◽  
María Luisa González-Fernández ◽  
Jessica Álvarez-Suárez ◽  
Jaime Sánchez-Lázaro ◽  
Marta Esteban-Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondroitin Sulfate ◽  
Inflammatory Cytokines ◽  
In Vitro Model ◽  
Promising Alternative ◽  
Cell Based Therapy ◽  
Vitro Model ◽  
Pro Inflammatory Cytokines

Abstract Introduction: Osteoarthritis (OA) is a degenerative joint disease which affects the whole joint structure. Many authors have focused on the factors responsible for the development of inflammatory processes involved in OA. Adipose tissue-derived mesenchymal stem cells (ASCs) represent a promising alternative of cell-based therapy strategy in the treatment of OA which could be combined with any other drug. Chondroitin sulfate plays a protective role in the joint based on the decrease of pro-inflammatory cytokines, thus having an important role in activating and inhibiting the metabolic pathways in chondrocytes. Aims: The effectiveness of chondroitin sulfate and ASCs combined in an in vitro model of OA has been evaluated in this study. Materials: Cytokines and factors which are involved in OA as well as specific cartilage gene expression after adding ASCs and chondroitin sulfate have been discussed in detail. Results: Our results show a decrease in the expression of all genes related to the pro-inflammatory cytokines analysed. Although there was no increase in the expression of the specific genes of the cartilage matrix, such as collagen type II and aggrecan. Conclusions: This study shows the effectiveness of association of ASCs and chondroitin sulfate for the treatment of OA.

scholarly journals Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 243 ◽  
Author(s):  
Monica Forni ◽  
Chiara Bernardini ◽  
Fausto Zamparini ◽  
Augusta Zannoni ◽  
Roberta Salaroli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Smooth Muscle Actin ◽  
Vascular Wall ◽  
Porous Scaffolds ◽  
Alpha Smooth Muscle Actin ◽  
Vitro Model ◽  
Highly Porous

Vascularization is a crucial factor when approaching any engineered tissue. Vascular wall–mesenchymal stem cells are an excellent in vitro model to study vascular remodeling due to their strong angiogenic attitude. This study aimed to demonstrate the angiogenic potential of experimental highly porous scaffolds based on polylactic acid (PLA) or poly-e-caprolactone (PCL) doped with calcium silicates (CaSi) and dicalcium phosphate dihydrate (DCPD), namely PLA-10CaSi-10DCPD and PCL-10CaSi-10DCPD, designed for the regeneration of bone defects. Vascular wall–mesenchymal stem cells (VW-MSCs) derived from pig thoracic aorta were seeded on the scaffolds and the expression of angiogenic markers, i.e. CD90 (mesenchymal stem/stromal cell surface marker), pericyte genes α-SMA (alpha smooth muscle actin), PDGFR-β (platelet-derived growth factor receptor-β), and NG2 (neuron-glial antigen 2) was evaluated. Pure PLA and pure PCL scaffolds and cell culture plastic were used as controls (3D in vitro model vs. 2D in vitro model). The results clearly demonstrated that the vascular wall mesenchymal cells colonized the scaffolds and were metabolically active. Cells, grown in these 3D systems, showed the typical gene expression profile they have in control 2D culture, although with some main quantitative differences. DNA staining and immunofluorescence assay for alpha-tubulin confirmed a cellular presence on both scaffolds. However, VW-MSCs cultured on PLA-10CaSi-10DCPD showed an individual cells growth, whilst on PCL-10CaSi-10DCPD scaffolds VW-MSCs grew in spherical clusters. In conclusion, vascular wall mesenchymal stem cells demonstrated the ability to colonize PLA and PCL scaffolds doped with CaSi-DCPD for new vessels formation and a potential for tissue regeneration.

scholarly journals Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia

2011 ◽  
Vol 2 (2) ◽  
pp. 12 ◽  
Author(s):  
Lisa McGinley ◽  
Jill McMahon ◽  
Padraig Strappe ◽  
Frank Barry ◽  
Mary Murphy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Model ◽  
Lentiviral Vector ◽  
Vitro Model

scholarly journals Neuron-Like Cells Generated from Human Umbilical Cord Lining-Derived Mesenchymal Stem Cells as a New In Vitro Model for Neuronal Toxicity Screening: Using Magnetite Nanoparticles as an Example

2019 ◽  
Vol 21 (1) ◽  
pp. 271 ◽  
Author(s):  
Uliana De Simone ◽  
Arsenio Spinillo ◽  
Francesca Caloni ◽  
Laura Gribaldo ◽  
Teresa Coccini
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Magnetite Nanoparticles ◽  
In Vitro Model ◽  
Immunofluorescent Staining ◽  
Human Umbilical Cord ◽  
Neuronal Toxicity ◽  
Vitro Model

The wide employment of iron nanoparticles in environmental and occupational settings underlines their potential to enter the brain. Human cell-based systems are recommended as relevant models to reduce uncertainty and to improve prediction of human toxicity. This study aimed at demonstrating the in vitro differentiation of the human umbilical cord lining-derived-mesenchymal stem cells (hCL-MSCs) into neuron-like cells (hNLCs) and the benefit of using them as an ideal primary cell source of human origin for the neuronal toxicity of Fe3O4NPs (magnetite-nanoparticles). Neuron-like phenotype was confirmed by: live morphology; Nissl body staining; protein expression of different neuronal-specific markers (immunofluorescent staining), at different maturation stages (i.e., day-3-early and day-8-full differentiated), namely β-tubulin III, MAP-2, enolase (NSE), glial protein, and almost no nestin and SOX-2 expression. Synaptic makers (SYN, GAP43, and PSD95) were also expressed. Fe3O4NPs determined a concentration- and time-dependent reduction of hNLCs viability (by ATP and the Trypan Blue test). Cell density decreased (20–50%) and apoptotic effects were detected at ≥10 μg/mL in both types of differentiated hNLCs. Three-day-differentiated hNLCs were more susceptible (toxicity appeared early and lasted for up to 48 h) than 8-day-differentiated cells (delayed effects). The study demonstrated that (i) hCL-MSCs easily differentiated into neuronal-like cells; (ii) the hNCLs susceptibility to Fe3O4NPs; and (iii) human primary cultures of neurons are new in vitro model for NP evaluation.

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