Expression profiling of long noncoding RNAs associated with vasculogenic mimicry in osteosarcoma

2019 ◽  
Vol 120 (8) ◽  
pp. 12473-12488 ◽  
Author(s):  
Ke Ren ◽  
Yicheng Ni ◽  
Xingjia Li ◽  
Chen Wang ◽  
Qing Chang ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Vasculogenic Mimicry ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas

scholarly journals The Effect of Botulinum Toxin Type A on Expression Profiling of Long Noncoding RNAs in Human Dermal Fibroblasts

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Ying-ying Miao ◽  
Juan Liu ◽  
Jie Zhu ◽  
Yan-ling Tao ◽  
Jia-an Zhang ◽  
...  
Keyword(s):  
Botulinum Toxin ◽  
Differentially Expressed Genes ◽  
Expression Profiling ◽  
Botulinum Toxin Type A ◽  
Noncoding Rnas ◽  
Botulinum Toxin Type ◽  
Dermal Fibroblasts ◽  
Long Noncoding Rnas ◽  
Human Dermal Fibroblasts ◽  
Qrt Pcr

Objective. This study was aimed at analyzing the expressions of long noncoding RNAs (lncRNAs) in Botulinum Toxin Type A (BoNTA) treated human dermal fibroblasts (HDFs) in vitro. Methods. We used RNA sequencing to characterize the lncRNAs and mRNAs transcriptome in the control and BoNTA treated group, in conjunction with application of GO (gene ontology) analysis and KEGG (kyoto encyclopedia of genes and genomes) analysis to delineate the alterations in gene expression. We also obtained quantitative real time polymerase chain reaction (qRT-PCR) to confirm some differentially expressed genes. Results. Numerous differentially expressed genes were observed by microarrays between the two groups. qRT-PCR confirmed the changes of six lncRNAs (RP11-517C16.2-001, FR271872, LOC283352, RP11-401E9.3, FGFR3P, and XXbac-BPG16N22.5) and nine mRNAs (NOS2, C13orf15, FOS, FCN2, SPINT1, PLAC8, BIRC5, NOS2, and COL19A1). Farther studies indicated that the downregulating effect of BoNTA on the expression of FGFR3P was time-related and the dosage of BoNTA at a range from 2.5 U/106 cells to 7.5 U/106 cells increased the expression of FGFR3P and COL19A1 in HDFs as well. Conclusion. The expression profiling of lncRNAs was visibly changed in BoNTA treated HDFs. Further studies should focus on several lncRNAs to investigate their functions in BoNTA treated HDFs and the underlying mechanisms.

Co-expression profiling of plasma miRNAs and long noncoding RNAs in gastric cancer patients

Gene ◽  
2019 ◽  
Vol 687 ◽  
pp. 135-142 ◽  
Author(s):  
Hamid Ghaedi ◽  
Mohammad Amin Nazer Mozaffari ◽  
Zaker Salehi ◽  
Hassan Ghasemi ◽  
Seyed Sajjad Zadian ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Expression Profiling ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Gastric Cancer Patients ◽  
Plasma Mirnas
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