Celastrol protects human retinal pigment epithelial cells against hydrogen peroxide mediated oxidative stress, autophagy, and apoptosis through sirtuin 3 signal pathway

2019 ◽  
Vol 120 (6) ◽  
pp. 10413-10420 ◽  
Author(s):  
Zhaojiang Du ◽  
Wen Zhang ◽  
Shengyu Wang ◽  
Jing Zhang ◽  
Jingang He ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Signal Pathway ◽  
Retinal Pigment Epithelial Cells ◽  
Sirtuin 3 ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

scholarly journals Pueraria lobata Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Nu Ri Kang ◽  
Bo-Jeong Pyun ◽  
Dong Ho Jung ◽  
Ik Soo Lee ◽  
Chan-Sik Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Epithelial Cells ◽  
Membrane Permeability ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Ros Generation ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

Background. Pueraria lobata is used in traditional Asian medicine to treat cardiovascular diseases, diarrhea, diabetes mellitus, and diabetic complications such as diabetic retinopathy. Oxidative stress in retinal pigment epithelial cells is implicated in the pathogenesis of retinopathy and age-related macular degeneration (AMD). Here, we evaluated whether the P. lobata extract can prevent cell death and decrease membrane permeability in oxidative stress-induced human retinal pigment epithelial cells. Methods. The effects of P. lobata extract on hydrogen peroxide- (H2O2-) induced oxidative stress were investigated using 2′,7′–dichlorofluorescin diacetate, western blotting, and immunohistochemistry in human retinal pigment epithelial cells. The effects of puerarin, daidzein, and daidzin isolated from P. lobata extract were also studied by determining cell death, reactive oxygen species (ROS) generation, and p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) phosphorylation. Results. Our results showed that the P. lobata extract inhibited ROS generation, suppressed the disruption of zonula occludens-1 (ZO-1), and reduced membrane permeability in H2O2-induced human retinal pigment epithelial cells. Additionally, the P. lobata extract prevented the inhibition of p38 MAPK and JNK phosphorylation. Conclusion. Our findings suggest that the P. lobata extract has the potential to prevent AMD development by inhibiting the mechanism underlying oxidative stress-mediated ocular disorders.

scholarly journals Iron prochelator BSIH protects retinal pigment epithelial cells against cell death induced by hydrogen peroxide

2008 ◽  
Vol 102 (12) ◽  
pp. 2130-2135 ◽  
Author(s):  
Louise K. Charkoudian ◽  
Tzvete Dentchev ◽  
Nina Lukinova ◽  
Natalie Wolkow ◽  
Joshua L. Dunaief ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells
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