Improved angiogenic activity of endothelial progenitor cell in diabetic patients treated with insulin plus metformin

2018 ◽  
Vol 120 (5) ◽  
pp. 7115-7124 ◽  
Author(s):  
Simin Asadian ◽  
Mahdi Alibabrdel ◽  
Nazanin Daei ◽  
Hadi Cheraghi ◽  
Seyedeh Maedeh Jafari ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Diabetic Patients ◽  
Endothelial Progenitor ◽  
Angiogenic Activity

scholarly journals Coenzyme Q10 Attenuates High Glucose-Induced Endothelial Progenitor Cell Dysfunction through AMP-Activated Protein Kinase Pathways

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Hsiao-Ya Tsai ◽  
Chih-Pei Lin ◽  
Po-Hsun Huang ◽  
Szu-Yuan Li ◽  
Jia-Shiong Chen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Coenzyme Q10 ◽  
High Glucose ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Heme Oxygenase 1 ◽  
Diabetic Patients ◽  
No Production ◽  
Endothelial Progenitor ◽  
Amp Activated Protein Kinase

Coenzyme Q10 (CoQ10), an antiapoptosis enzyme, is stored in the mitochondria of cells. We investigated whether CoQ10 can attenuate high glucose-induced endothelial progenitor cell (EPC) apoptosis and clarified its mechanism. EPCs were incubated with normal glucose (5 mM) or high glucose (25 mM) enviroment for 3 days, followed by treatment with CoQ10 (10 μM) for 24 hr. Cell proliferation, nitric oxide (NO) production, and JC-1 assay were examined. The specific signal pathways of AMP-activated protein kinase (AMPK), eNOS/Akt, and heme oxygenase-1 (HO-1) were also assessed. High glucose reduced EPC functional activities, including proliferation and migration. Additionally, Akt/eNOS activity and NO production were downregulated in high glucose-stimulated EPCs. Administration of CoQ10 ameliorated high glucose-induced EPC apoptosis, including downregulation of caspase 3, upregulation of Bcl-2, and increase in mitochondrial membrane potential. Furthermore, treatment with CoQ10 reduced reactive oxygen species, enhanced eNOS/Akt activity, and increased HO-1 expression in high glucose-treated EPCs. These effects were negated by administration of AMPK inhibitor. Transplantation of CoQ10-treated EPCs under high glucose conditions into ischemic hindlimbs improved blood flow recovery. CoQ10 reduced high glucose-induced EPC apoptosis and dysfunction through upregulation of eNOS, HO-1 through the AMPK pathway. Our findings provide a potential treatment strategy targeting dysfunctional EPC in diabetic patients.

Aliskiren directly improves endothelial progenitor cell function from Type II diabetic patients

2016 ◽  
Vol 46 (6) ◽  
pp. 544-554 ◽  
Author(s):  
Ting-Ting Chang ◽  
Tao-Cheng Wu ◽  
Po-Hsun Huang ◽  
Jia-Shiong Chen ◽  
Liang-Yu Lin ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Cell Function ◽  
Diabetic Patients ◽  
Type Ii ◽  
Endothelial Progenitor

scholarly journals Preserved endothelial progenitor cell angiogenic activity in African American essential hypertensive patients

2017 ◽  
Vol 33 (3) ◽  
pp. 392-401 ◽  
Author(s):  
Seo Rin Kim ◽  
Alfonso Eirin ◽  
Sandra M S Herrmann ◽  
Ahmed Saad ◽  
Luis A Juncos ◽  
...  
Keyword(s):  
African American ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Endothelial Progenitor ◽  
Hypertensive Patients ◽  
Angiogenic Activity

scholarly journals Downregulation of MicroRNA-130a Contributes to Endothelial Progenitor Cell Dysfunction in Diabetic Patients via Its Target Runx3

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68611 ◽  
Author(s):  
Shu Meng ◽  
Jiatian Cao ◽  
Xiaoping Zhang ◽  
Yuqi Fan ◽  
Lu Fang ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Diabetic Patients ◽  
Endothelial Progenitor ◽  
Cell Dysfunction
Sign in / Sign up

Export Citation Format

Share Document