Computational optimization of in vitro parameters for di‐(2‐ethylhexyl) phthalate production from Anabaena circinalis

2018 ◽  
Vol 120 (1) ◽  
pp. 790-798 ◽  
Author(s):  
Aratrika Sarkar ◽  
Gowri shankar Bagavananthem Andavan
Keyword(s):  
Computational Optimization ◽  
Ethylhexyl Phthalate ◽  
Anabaena Circinalis

In vitro metabolism of di(2-ethylhexyl) phthalate (DEHP) by various tissues and cytochrome P450s of human and rat

2012 ◽  
Vol 26 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Kyoungju Choi ◽  
Hyun Joo ◽  
Jerry L. Campbell ◽  
Rebecca A. Clewell ◽  
Melvin E. Andersen ◽  
...  
Keyword(s):  
Cytochrome P450s ◽  
In Vitro Metabolism ◽  
Ethylhexyl Phthalate

Hydrolysis of di(2-ethylhexyl) phthalate in humans, monkeys, dogs, rats, and mice: An in vitro analysis using liver and intestinal microsomes

2019 ◽  
Vol 54 ◽  
pp. 237-242 ◽  
Author(s):  
Nobumitsu Hanioka ◽  
Takashi Isobe ◽  
Susumu Ohkawara ◽  
Sadayuki Ochi ◽  
Toshiko Tanaka-Kagawa ◽  
...  
Keyword(s):  
Intestinal Microsomes ◽  
Vitro Analysis ◽  
Rats And Mice ◽  
Ethylhexyl Phthalate ◽  
Hydrolysis Of ◽  
In Vitro Analysis

In vitro exposure to di-(2-ethylhexyl) phthalate (DEHP) stimulates spontaneous feline uterine contractions

2020 ◽  
Vol 44 (4) ◽  
pp. 919-927
Author(s):  
Ruhi KABAKÇI ◽  
Hasan Ceyhun MACUN ◽  
Ebru YILDIRIM ◽  
İbrahim Mert POLAT ◽  
Taha Burak ELİFOĞLU
Keyword(s):  
Uterine Contractions ◽  
In Vitro Exposure ◽  
Ethylhexyl Phthalate

Encircling granulosa cells protects against di-(2-ethylhexyl)phthalate-induced apoptosis in rat oocytes cultured in vitro

Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 203-213 ◽  
Author(s):  
Anima Tripathi ◽  
Vivek Pandey ◽  
A.N. Sahu ◽  
Alok K. Singh ◽  
Pawan K. Dubey
Keyword(s):  
Oxidative Stress ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Granulosa Cells ◽  
Mitochondrial Membrane ◽  
Dependent Manner ◽  
Apoptotic Markers ◽  
Induced Apoptosis ◽  
Ethylhexyl Phthalate

SummaryThe present study investigated if the presence of encircling granulosa cells protected against di(2-ethylhexyl)phthalate (DEHP)-induced oxidative stress in rat oocytes cultured in vitro. Denuded oocytes and cumulus–oocyte complexes (COCs) were treated with or without various doses of DEHP (0.0, 25.0, 50.0, 100, 200, 400 and 800 μM) in vitro. Morphological apoptotic changes, levels of oxidative stress and reactive oxygen species (ROS), mitochondrial membrane potential, and expression levels of apoptotic markers (Bcl2, Bax, cytochrome c) were analyzed. Our results showed that DEHP induced morphological apoptotic changes in a dose-dependent manner in denuded oocytes cultured in vitro. The effective dose of DEHP (400 µg) significantly (P>0.05) increased oxidative stress by elevating ROS levels and the mitochondrial membrane potential with higher mRNA expression and protein levels of apoptotic markers (Bax, cytochrome c). Encircling granulosa cells protected oocytes from DEHP-induced morphological changes, increased oxidative stress and ROS levels, as well as increased expression of apoptotic markers. Taken together our data suggested that encircling granulosa cells protected oocytes against DEHP-induced apoptosis and that the presence of granulosa cells could act positively towards the survival of oocytes under in vitro culture conditions and may be helpful during assisted reproductive technique programmes.

scholarly journals The Effects of Mono-(2-Ethylhexyl) Phthalate (MEHP) on Human Estrogen Receptor (hER) and Androgen Receptor (hAR) by YES/YAS In Vitro Assay

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1558 ◽  
Author(s):  
Kim ◽  
Park ◽  
Kim ◽  
Kim
Keyword(s):  
Estrogen Receptor ◽  
Androgen Receptor ◽  
Endocrine System ◽  
Yeast Cells ◽  
Signal Recovery ◽  
Vitro Assay ◽  
Recovery Test ◽  
Human Estrogen Receptor ◽  
Ethylhexyl Phthalate

Endocrine active compounds with structural similarities to natural hormones such as 17β-estradiol (E2) and androgen are suspected to affect the human endocrine system by inducing hormone-dependent effects. This study aimed to detect the (anti-)estrogenic and (anti-)androgenic activities of mono-(2-ethylhexyl) phthalate (MEHP) by yeast estrogen/androgen bioassay (YES/YAS). In addition, the mechanism and uptake of MEHP to receptors during agonistic and antagonistic activities were investigated through the activation signal recovery test and chromatographic analysis using liquid chromatography and tandem mass spectrometry (LC-MS/MS). Estrogenic and androgenic activities of MEHP were not observed. However, MEHP exhibited anti-estrogenic (IC50 = 125 μM) and anti-androgenic effects (IC50 = 736 μM). It was confirmed that these inhibitory effects of MEHP were caused by receptor-mediated activity of the estrogen receptor and non-receptor-mediated activity of the androgen receptor in an activation signal recovery test. When IC50 concentrations of anti-estrogenic and androgenic activity of MEHP were exposed to yeast cells, the uptake concentration observed was 0.0562 ± 0.0252 μM and 0.143 ± 0.0486 μM by LC-MS/MS analysis.

scholarly journals Mono-(2-ethylhexyl) phthalate stimulates basal steroidogenesis by a cAMP-independent mechanism in mouse gonadal cells of both sexes

Reproduction ◽  
2008 ◽  
Vol 135 (5) ◽  
pp. 693-703 ◽  
Author(s):  
David Gunnarsson ◽  
Per Leffler ◽  
Emelie Ekwurtzel ◽  
Gunilla Martinsson ◽  
Kui Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Developmental Disorders ◽  
Hormone Sensitive Lipase ◽  
Dependent Manner ◽  
Simultaneous Treatment ◽  
Concentration Dependent Manner ◽  
Steroid Synthesis ◽  
Independent Mechanism ◽  
Ethylhexyl Phthalate

Phthalates are widely used as plasticizers in a number of daily-life products. In this study, we investigated the influence of mono-(2-ethylhexyl) phthalate (MEHP), the active metabolite of the frequently used plasticizer di-(2-ethylhexyl) phthalate (DEHP), on gonadal steroidogenesisin vitro. MEHP (25–100 μM) stimulated basal steroid synthesis in a concentration-dependent manner in immortalized mouse Leydig tumor cells (MLTC-1). The stimulatory effect was also detected in KK-1 granulosa tumor cells. MEHP exposure did not influence cAMP or StAR protein levels and induced a gene expression profile of key steroidogenic proteins different from the one induced by human chorionic gonadotropin (hCG). Simultaneous treatment with MEHP and a p450scc inhibitor (aminoglutethimide) indicated that MEHP exerts its main stimulatory effect prior to pregnenolone formation. MEHP (10–100 μM) up-regulated hormone-sensitive lipase and 3-hydroxy-3-methylglutaryl coenzyme A reductase, suggesting that MEHP increases the amount of cholesterol available for steroidogenesis. Our data suggest that MEHP, besides its known inhibitory effect on hCG action, can directly stimulate gonadal steroidogenesis in both sexes through a cAMP- and StAR-independent mechanism. The anti-steroidogenic effect of DEHP has been proposed to cause developmental disorders such as hypospadias and cryptorchidism, whereas a stimulation of steroid synthesis may prematurely initiate the onset of puberty and theoretically affect the hypothalamic–pituitary–gonadal axis.

scholarly journals Effect of mono-(2-ethylhexyl) phthalate on human and mouse fetal testis: In vitro and in vivo approaches

2012 ◽  
Vol 261 (1) ◽  
pp. 97-104 ◽  
Author(s):  
V. Muczynski ◽  
J.P. Cravedi ◽  
A. Lehraiki ◽  
C. Levacher ◽  
D. Moison ◽  
...  
Keyword(s):  
Fetal Testis ◽  
Ethylhexyl Phthalate ◽  
Human And Mouse
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