A siRNA‐based method for efficient silencing of PYROXD1 gene expression in the colon cancer cell line HCT116

2019 ◽  
Vol 120 (12) ◽  
pp. 19310-19317
Author(s):  
Samira Shabani ◽  
Frouzandeh Mahjoubi ◽  
Mohammad A. Moosavi
2011 ◽  
Vol 14 (10) ◽  
pp. 1107-1117 ◽  
Author(s):  
Mehmet Turktekin ◽  
Ece Konac ◽  
H. Ilke Onen ◽  
Ebru Alp ◽  
Akin Yilmaz ◽  
...  

Cytokine ◽  
2017 ◽  
Vol 97 ◽  
pp. 66-72 ◽  
Author(s):  
Amal F. Gharib ◽  
Sally M. Shalaby ◽  
Nermin Raafat ◽  
Walaa M.S. Fawzy ◽  
Nabila H. Abdel Hakim

Author(s):  
Timur KOCA ◽  
Ersin AKINCI ◽  
Betül ÜNAL ◽  
Cumhur İbrahim BAŞSORGUN ◽  
Gülsüm Özlem ELPEK

2020 ◽  
Vol 4 (2) ◽  
pp. 37
Author(s):  
Süreyya Bozkurt ◽  
Filiz Yarimcan ◽  
Hüseyin Ayhan ◽  
Hacer Kotan ◽  
Hüma Tuğçe Sezgin ◽  
...  

Cancer is a disease arising from DNA alterations that dysregulate gene structure and function. These deregulated genes can also play a role in tumor invasion and metastasis or resistance to treatment. In this study, we determined the gene expression during transcription of PTGS2 (Prostaglandin-endoperoxide synthase 2), MAGE-A3 (Melanoma-associated antigen 3), CALR (Calreticulin), KRT19 (Cytokeratin 19), and TMPRSS4 (Transmembrane protease, serine 4) in HCT116 colon cancer cell line and PC3 prostate cancer cell line. After RNA isolation and cDNA conversion, DNA amplification was performed with Real-Time PCR. We determined the altered transcriptional expression level of those genes. In HCT116 colon cancer cell line, expression of the TMPRSS4 gene, MAGEA3 gene and KRT19 gene was found as increased and expression of the CALR gene and the PTGS2 gene was found as decreased. Especially a 93.70-fold increase in expression of the KRT19 gene was found in HCT116 colon cancer cell line. In PC3 prostate cancer cell lines, TMPRS4 gene expression and MAGEA3 gene expression were found as increased. But there was 50 fold decrease in PTGS2 gene expression.


1998 ◽  
Vol 114 ◽  
pp. A577
Author(s):  
D.P. Chauhan ◽  
Q.H. Yang ◽  
J.M. Carethers ◽  
C.L. Chang ◽  
C.R. Boland

2000 ◽  
Vol 118 (4) ◽  
pp. A46-A47
Author(s):  
Mitsunori Honda ◽  
Toshifumi Ashida ◽  
Mikihiro Fujiya ◽  
Kaori Fujiya ◽  
Jiro Watari ◽  
...  

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