Effects of various culture conditions on pluripotent stem cell derivation from chick embryos

2018 ◽  
Vol 119 (8) ◽  
pp. 6325-6336 ◽  
Author(s):  
Maryam Farzaneh ◽  
Masoumeh Zare ◽  
Seyedeh‐Nafiseh Hassani ◽  
Hossein Baharvand
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Culture Conditions ◽  
Chick Embryos ◽  
Stem Cell Derivation

scholarly journals Stem cell culture conditions and stability: a joint workshop of the PluriMes Consortium and Pluripotent Stem Cell Platform

2019 ◽  
Vol 14 (3) ◽  
pp. 243-255 ◽  
Author(s):  
Glyn N Stacey ◽  
Peter W Andrews ◽  
Ivana Barbaric ◽  
Charlotta Boiers ◽  
Amit Chandra ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Culture Conditions ◽  
Stem Cell Culture ◽  
Joint Workshop ◽  
Cell Culture Conditions

scholarly journals Influence of basal media composition on barrier fidelity within human pluripotent stem cell-derived blood-brain barrier models

2021 ◽  
Author(s):  
Emma Neal ◽  
Ketaki Katdare ◽  
Yajuan Shi ◽  
Nicholas Marinelli ◽  
Kameron Hagerla ◽  
...  
Keyword(s):  
Stem Cell ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cell ◽  
Molecular Transport ◽  
Culture Conditions ◽  
Brain Barrier ◽  
Media Composition ◽  
Blood Brain ◽  
Basal Media

It is increasingly recognized that brain microvascular endothelial cells (BMECs), the principle component of the blood-brain barrier (BBB), are highly sensitive to soluble cues from both the bloodstream and the brain. This concept extends in vitro, where the extracellular milieu can also influence BBB properties in cultured cells. However, the extent to which baseline culture conditions can affect BBB properties in vitro remains unclear, which has implications for model variability and reproducibility, as well as downstream assessments of molecular transport and disease phenotypes. Here, we explore this concept by examining BBB properties within human induced pluripotent stem cell (iPSC)-derived BMEC-like cells cultured under serum-free conditions in different basal media with fully defined compositions. We demonstrate notable differences in both passive and active BBB properties as a function of basal media composition. Further, RNA sequencing and phosphoproteome analyses revealed alterations to various signaling pathways in response to basal media differences. Overall, our results demonstrate that baseline culture conditions can have a profound influence on the performance of in vitro BBB models, and these effects should be considered when designing experiments that utilize such models for basic research and preclinical assays.

scholarly journals A Novel Feeder-Free Culture System for Human Pluripotent Stem Cell Culture and Induced Pluripotent Stem Cell Derivation

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76205 ◽  
Author(s):  
Sanna Vuoristo ◽  
Sanna Toivonen ◽  
Jere Weltner ◽  
Milla Mikkola ◽  
Jarkko Ustinov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Culture System ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Culture ◽  
Stem Cell Derivation ◽  
Induced Pluripotent

scholarly journals NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells

2022 ◽  
Vol 15 (1) ◽  
Author(s):  
Seo Young Kim ◽  
Jihye Choi ◽  
Junhee Roh ◽  
Chul Hoon Kim
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Culture Conditions ◽  
Mural Cells ◽  
Brain Pericytes ◽  
Specific Uptake ◽  
Induced Pluripotent ◽  
Anatomical Information

AbstractIn the CNS, pericytes are important for maintaining the blood–brain barrier (BBB) and for controlling blood flow. Recently, several methods were suggested for the differentiation of human pluripotent stem cells (hPSCs) into brain mural cells, specifically pericytes or vascular smooth muscle cells (vSMCs). Unfortunately, identifying the pericytes from among such hPSC-derived mural cells has been challenging. This is due both to the lack of pericyte-specific markers and to the loss of defining anatomical information inherent to culture conditions. We therefore asked whether NeuroTrace 500/525, a newly developed dye that shows cell-specific uptake into pericytes in the mouse brain, can help identify human induced pluripotent stem cell (hiPSC)-derived brain pericyte-like cells. First, we found that NeuroTrace 500/525 specifically stains primary cultured human brain pericytes, confirming its specificity in vitro. Second, we found that NeuroTrace 500/525 specifically labels hiPSC-derived pericyte-like cells, but not endothelial cells or vSMCs derived from the same hiPSCs. Last, we found that neuroectoderm-derived vSMCs, which have pericyte-like features, also take up NeuroTrace 500/525. These data indicate NeuroTrace 500/525 is useful for identifying pericyte-like cells among hiPSC-derived brain mural cells.

scholarly journals Ca2+-Currents in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Effects of Two Different Culture Conditions

2016 ◽  
Vol 7 ◽  
Author(s):  
Ahmet U. Uzun ◽  
Ingra Mannhardt ◽  
Kaja Breckwoldt ◽  
András Horváth ◽  
Silke S. Johannsen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Culture Conditions ◽  
Induced Pluripotent
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