Inhibition of Endoplasmic Reticulum Stress Apoptosis by Estrogen Protects Human Umbilical Vein Endothelial Cells Through the PI3 Kinase-Akt Signaling Pathway

2017 ◽  
Vol 118 (12) ◽  
pp. 4568-4574 ◽  
Author(s):  
Qing Su ◽  
Yu Wang ◽  
Xin Yang ◽  
Xiao-Dong Li ◽  
Yong-Fen Qi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Signaling Pathway ◽  
Umbilical Vein ◽  
Akt Signaling ◽  
Pi3 Kinase ◽  
Akt Signaling Pathway ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals The Effect and Mechanism of Emodin on the NO Secretion of Human Umbilical Vein Endothelial Cells (HUVECs) Induced by High Glucose

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Yajia Li ◽  
Qiangxiang Li ◽  
Chunyan Xie ◽  
Yanfei Huang ◽  
Limin Jia
Keyword(s):  
Endothelial Cells ◽  
Protein Expression ◽  
Signaling Pathway ◽  
High Glucose ◽  
Umbilical Vein ◽  
Akt Signaling ◽  
High Dose ◽  
Akt Signaling Pathway ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Objective. To investigate the effects of emodin on nitric oxide (NO) secretion induced by high glucose in human umbilical vein endothelial cells (HUVECs) through the p-Akt signaling pathway. Methods. Sensitivity of cells to emodin was determined by MTT assay to establish the experimental concentrations; then, HUVECs were treated with high-dose (33.3 mmol/L) glucose (HG), HG + emodin (HG + E), HG + the Akt phosphorylation inhibitor LY294002 (HG + LY), or HG + E + LY. The p-Akt (Ser 473) expression in 48 h was analyzed using Western blot. NO effect on the secretion of HUVECs was analyzed using nitrate reductase assay. Results. The sensitive emodin concentration for HUVECs growth was 10 mol/L (P<0.05). Compared with the HG group, NO secretion was significantly higher in the HG + E group (P<0.05), whereas it was lowest in the HG + LY group (P<0.05). Compared with the HG + LY group, NO secretion was increased in the HG + E + LY group (P<0.05). The p-Akt protein expression was decreased in the HG + LY group when compared to the HG group (P<0.05), while it significantly increased in the HG + E group (P<0.05). Compared with HG + LY group, p-Akt protein expression was significantly higher in the HG + E + LY group (P<0.05). Conclusion. Emodin could improve the NO secretion of HUVECs by high glucose through the p-Akt signaling pathway.

Paeoniflorin prevents endoplasmic reticulum stress-associated inflammation in lipopolysaccharide-stimulated human umbilical vein endothelial cells via the IRE1α/NF-κB signaling pathway

2018 ◽  
Vol 9 (4) ◽  
pp. 2386-2397 ◽  
Author(s):  
Juan Chen ◽  
Minghua Zhang ◽  
Maomao Zhu ◽  
Junfei Gu ◽  
Jie Song ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Er Stress ◽  
Signaling Pathway ◽  
Umbilical Vein ◽  
Vascular Inflammation ◽  
Inhibitory Effect ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Paeoniflorin has inhibitory effect on ER stress-associated vascular inflammation.

Endoplasmic reticulum stress in human umbilical vein endothelial cells from pre-gestational maternal obesity

Placenta ◽  
2017 ◽  
Vol 51 ◽  
pp. 116
Author(s):  
R. Villalobos-Labra ◽  
R. Salsoso ◽  
M. Subiabre ◽  
L. Silva ◽  
M. Farías-Jofré ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Maternal Obesity ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells
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