Type 2 Diabetes Inhibited Human Mesenchymal Stem Cells Angiogenic Response by Over-Activity of the Autophagic Pathway

2017 ◽  
Vol 118 (6) ◽  
pp. 1518-1530 ◽  
Author(s):  
Aysa Rezabakhsh ◽  
Omid Cheraghi ◽  
Alireza Nourazarian ◽  
Mehdi Hassanpour ◽  
Masoumeh Kazemi ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Angiogenic Response

scholarly journals Secretome from Human Mesenchymal Stem Cells-Derived Endothelial Cells Promotes Wound Healing in a Type-2 Diabetes Mouse Model

2022 ◽  
Vol 23 (2) ◽  
pp. 941
Author(s):  
Valeska Ormazabal ◽  
Estefanía Nova-Lampeti ◽  
Daniela Rojas ◽  
Felipe A. Zúñiga ◽  
Carlos Escudero ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Healing Process ◽  
Human Mesenchymal Stem Cells ◽  
Wound Healing Process ◽  
Diabetes Mouse

Tissue regeneration is often impaired in patients with metabolic disorders such as diabetes mellitus and obesity, exhibiting reduced wound repair and limited regeneration capacity. We and others have demonstrated that wound healing under normal metabolic conditions is potentiated by the secretome of human endothelial cell-differentiated mesenchymal stem cells (hMSC-EC). However, it is unknown whether this effect is sustained under hyperglycemic conditions. In this study, the wound healing effect of secretomes from undifferentiated human mesenchymal stem cells (hMSC) and hMSC-EC in a type-2 diabetes mouse model was analyzed. hMSC were isolated from human Wharton’s jelly and differentiated into hMSC-EC. hMSC and hMSC-EC secretomes were analyzed and their wound healing capacity in C57Bl/6J mice fed with control (CD) or high fat diet (HFD) was evaluated. Our results showed that hMSC-EC secretome enhanced endothelial cell proliferation and wound healing in vivo when compared with hMSC secretome. Five soluble proteins (angiopoietin-1, angiopoietin-2, Factor de crecimiento fibroblástico, Matrix metallopeptidase 9, and Vascular Endothelial Growth Factor) were enriched in hMSC-EC secretome in comparison to hMSC secretome. Thus, the five recombinant proteins were mixed, and their pro-healing property was evaluated in vitro and in vivo. Functional analysis demonstrated that a cocktail of these proteins enhanced the wound healing process similar to hMSC-EC secretome in HFD mice. Overall, our results show that hMSC-EC secretome or a combination of specific proteins enriched in the hMSC-EC secretome enhanced wound healing process under hyperglycemic conditions.

Human mesenchymal stem cells alter the gene profile of monocytes from patients with Type 2 diabetes and end-stage renal disease

2016 ◽  
Vol 11 (2) ◽  
pp. 145-158 ◽  
Author(s):  
Andrea F Wise ◽  
Timothy M Williams ◽  
Stephen Rudd ◽  
Christine A Wells ◽  
Peter G Kerr ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Renal Disease ◽  
End Stage Renal Disease ◽  
Human Mesenchymal Stem Cells ◽  
Gene Profile ◽  
Stage Renal Disease ◽  
End Stage

scholarly journals Treatment of refractory secondary hemophagocytic lymphohistiocytosis with umbilical cord mesenchymal stem cells

2019 ◽  
Vol 47 (5) ◽  
pp. 2135-2144
Author(s):  
Xiaoyang Yang ◽  
Shufang Zhang ◽  
Denggao Huang ◽  
Zhiming Wang ◽  
Xiaoxia Chen ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Hemophagocytic Lymphohistiocytosis ◽  
Third Party ◽  
Inflammatory Factors ◽  
Cell Bank ◽  
First Case

Hemophagocytic lymphohistiocytosis (HLH) is an aggressive and life-threatening syndrome of excessive immune activation. Mesenchymal stem cells (MSCs) generate an immunosuppressive microenvironment by secreting cytokines and have been used to treat autoimmune diseases. We report the first case of refractory secondary HLH treated with umbilical cord MSCs. A 52-year-old Chinese female patient with a history of type 2 diabetes was diagnosed with refractory secondary HLH based upon the HLH-2004 protocol and was treated by infusion of third-party umbilical cord MSCs (1.4 × 106 cells/kg of body weight, 70 × 106 cells in total) from the stem cell bank of Hainan Province. Body temperature recovered to normal on the sixth day after infusion with umbilical cord MSCs, and the levels of inflammatory factors macrophage inflammatory protein (MIP)-1α, interleukin (IL)-12p70, stromal cell-derived factor (SDF)-1α, and IL-7 decreased significantly. Blood glucose levels were significantly lower than before treatment, and the amount of insulin needed was significantly reduced. Umbilical cord MSCs can relieve the symptoms of refractory secondary HLH and have a therapeutic effect on insulin resistance in type 2 diabetes mellitus.

The safety of bone-marrow derived mesenchymal stem cells in patients with type 2 diabetes mellitus

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S82 ◽  
Author(s):  
P. Adorable-Wagan ◽  
S. Bernal ◽  
D. Lavilles ◽  
M. De Vera
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Type 2 Diabetes Mellitus

Impact of type 2 diabetes mellitus on the immunoregulatory characteristics of adipose tissue-derived mesenchymal stem cells

2021 ◽  
Vol 140 ◽  
pp. 106072
Author(s):  
Nourhan Abu-Shahba ◽  
Marwa Mahmoud ◽  
Alaa Mohammed El-Erian ◽  
Mohamed Ibrahim Husseiny ◽  
Ghada Nour-Eldeen ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Type 2 Diabetes Mellitus ◽  
Adipose Tissue

scholarly journals Therapeutic efficacy of umbilical cord-derived mesenchymal stem cells in patients with type 2 diabetes

2015 ◽  
Vol 9 (5) ◽  
pp. 1623-1630 ◽  
Author(s):  
LI-XUE GUAN ◽  
HUI GUAN ◽  
HAI-BO LI ◽  
CUI-AI REN ◽  
LIN LIU ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Therapeutic Efficacy

scholarly journals Long term effect and safety of Wharton's jelly-derived mesenchymal stem cells on type 2 diabetes

2016 ◽  
Vol 12 (3) ◽  
pp. 1857-1866 ◽  
Author(s):  
Jianxia Hu ◽  
Yangang Wang ◽  
Huimin Gong ◽  
Chundong Yu ◽  
Caihong Guo ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Term Effect ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Long Term Effect
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