Spacial and Temporal Patterns of Gene Expression After Cardiac MEK1 Gene Transfer Improve Post-Infarction Remodeling Without Inducing Global Hypertrophy

2016 ◽  
Vol 118 (4) ◽  
pp. 775-784 ◽  
Author(s):  
Yanying Fan ◽  
Yi-Lin Yang ◽  
Che-Chung Yeh ◽  
Michael J. Mann
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Temporal Patterns

scholarly journals Airway gene transfer in a non-human primate: Lentiviral gene expression in marmoset lungs

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
N. Farrow ◽  
D. Miller ◽  
P. Cmielewski ◽  
M. Donnelley ◽  
R. Bright ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Human Primate

Gene Expression Analysis of Ectopic Bone Formation Induced by Electroporatic Gene Transfer of BMP4

2006 ◽  
Vol 111 (2) ◽  
pp. 231-242 ◽  
Author(s):  
Satoshi Kotajima ◽  
Koshi N. Kishimoto ◽  
Munenori Watanuki ◽  
Masahito Hatori ◽  
Shoichi Kokubun
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Bone Formation ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Ectopic Bone Formation ◽  
Ectopic Bone

scholarly journals Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain

FEBS Letters ◽  
1998 ◽  
Vol 433 (1-2) ◽  
pp. 37-40 ◽  
Author(s):  
Makoto Sawada ◽  
Fumihiro Imai ◽  
Hiromi Suzuki ◽  
Motoharu Hayakawa ◽  
Tetsuo Kanno ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Microglial Cell ◽  
Mammalian Brain ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals Breaching the Delivery Barrier: Chemical and Physical Airway Epithelium Disruption Strategies for Enhancing Lentiviral-Mediated Gene Therapy

2021 ◽  
Vol 12 ◽  
Author(s):  
Alexandra McCarron ◽  
Nigel Farrow ◽  
Patricia Cmielewski ◽  
Emma Knight ◽  
Martin Donnelley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Barrier Properties ◽  
Airway Epithelial Cells ◽  
Viral Gene ◽  
Airway Epithelial ◽  
Expression Levels ◽  
Epithelial Disruption ◽  
Gene Expression Levels

The lungs have evolved complex physical, biological and immunological defences to prevent foreign material from entering the airway epithelial cells. These mechanisms can also affect both viral and non-viral gene transfer agents, and significantly diminish the effectiveness of airway gene-addition therapies. One strategy to overcome the physical barrier properties of the airway is to transiently disturb the integrity of the epithelium prior to delivery of the gene transfer vector. In this study, chemical (lysophosphatidylcholine, LPC) and physical epithelium disruption using wire abrasion were compared for their ability to improve airway-based lentiviral (LV) vector mediated transduction and reporter gene expression in rats. When luciferase expression was assessed at 1-week post LV delivery, LPC airway conditioning significantly enhanced gene expression levels in rat lungs, while a long-term assessment in a separate cohort of rats at 12 months revealed that LPC conditioning did not improve gene expression longevity. In rats receiving physical perturbation to the trachea prior to gene delivery, significantly higher LacZ gene expression levels were found when compared to LPC-conditioned or LV-only control rats when evaluated 1-week post gene transfer. This proof-of-principle study has shown that airway epithelial disruption strategies based on physical perturbation substantially enhanced LV-mediated airway gene transfer in the trachea.

Abstract 249: Delayed Gene Transfer Increases Transgene Expression in Vein Grafts

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Liang Du ◽  
Jingwan Zhang ◽  
Alexander Clowes ◽  
David Dichek
Keyword(s):  
Gene Expression ◽  
Blood Flow ◽  
Gene Transfer ◽  
Transgene Expression ◽  
Ex Vivo ◽  
Arterial Blood Flow ◽  
Vein Grafts ◽  
Arterial Blood ◽  
En Face

Background Autogenous vein grafts are effective therapies for obstructive arterial disease. However, their long-term utility is limited by stenosis and occlusion. Genetic engineering of veins that prevents intimal hyperplasia and atherosclerosis could significantly improve the clinical utility of vein grafts. We recently reported that a helper-dependent adenoviral vector (HDAd) reduces atherosclerosis 4 wks after gene transfer in fat-fed rabbits and can express a therapeutic transgene (apo AI) in normal rabbit carotids for at least 48 wks. Use of HDAd for vein graft gene therapy will depend on achievement of similarly high and persistent transgene expression in grafted veins. Hypothesis We tested the hypothesis that Ad-mediated transgene expression in grafted veins (at an early time point) can be increased by varying the timing of gene transfer. Methods Rabbit external jugular veins were transduced by exposure to a beta galactosidase (b-gal)-expressing Ad: in situ either without (a) or with (b) immediate arterial grafting; c) ex vivo with grafting after overnight incubation with Ad; d) in vivo immediately after grafting and e) in vivo 4 wks after grafting (n = 6 - 19 veins/group). Transgene expression was measured in veins removed 3 d after Ad exposure by PCR quantitation of b-gal mRNA and by en-face planimetry of blue-stained area. Results B-gal transgene expression was higher in ungrafted veins than in veins grafted immediately after gene transfer (84 ± 17 vs 9.4 ± 2.0 arbitrary units (AU); P < 0.0001). Overnight incubation of veins with Ad increased gene expression ex vivo by 10-fold but neither this nor performing vector infusion immediately after grafting improved gene expression (11 ± 4.7 and 9.1 ± 1.8 AU; P > 0.9 for both vs immediately grafted veins). Delaying gene transfer until 4 wks after grafting significantly increased gene expression, to a level equivalent to transgene expression in ungrafted veins (61 ± 11 AU; P = 0.3 vs ungrafted veins). En face planimetry yielded similar results. Conclusions Exposure of a transduced vein to arterial blood flow is associated with significant loss of transgene expression. Transgene expression in grafted veins is significantly higher when gene transfer is performed 4 wks after exposure of the vein to arterial blood flow.

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