The Lipophilic Vitamin C Derivative, 6-O-Palmitoylascorbate Protects Human Keratinocytes and 3D-Human Skin Equivalents Against X-Ray-Induced Oxidative Stress and Apoptosis More Markedly Than L-Ascorbic Acid

2016 ◽  
Vol 118 (2) ◽  
pp. 318-329 ◽  
Author(s):  
Li Xiao ◽  
Nobuhiko Miwa
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Human Skin ◽  
Human Keratinocytes ◽  
X Ray ◽  
Skin Equivalents

scholarly journals Hydrogen-Generating Silica Material Prevents UVA-Ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 76
Author(s):  
Li Xiao ◽  
Mai Mochizuki ◽  
Taka Nakahara ◽  
Nobuhiko Miwa
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Annexin V ◽  
Human Keratinocytes ◽  
Absorption Capacity ◽  
Human Cells ◽  
Skin Equivalents ◽  
Type Iv ◽  
Silica Material ◽  
3D Skin

Ultraviolet-A (UVA) irradiation induces harmful effects on skin cells and accelerates skin aging through oxidative stress. In this study, the effects of a hydrogen-generating silica material named ULH-002 against UVA injuries in human cells and 3D skin equivalents were investigated. The oxygen radical absorption capacity (ORAC) assay showed that both freshly prepared ULH-002 solutions and 7-day-old solutions exhibited equal peroxyl radical (ROO·) scavenging activities concentration-dependently. CellROX® green/orange staining showed that ULH-002 could reduce UVA-induced oxidative stress in human keratinocytes HaCaT and human gingival fibroblasts (HGFs). ULH-002 significantly prevented UVA-induced apoptotic/necrotic cell death and cell-viability decline in HGFs and keratinocytes, as shown by Annexin V/PI apoptosis assay and PrestoBlue assay, respectively. Immunostaining showed that ULH-002 prevented the UVA-induced deterioration of expression of both type IV and I collagens in the 3D skin equivalents, and similarly in monolayer HGFs. UVA-enhanced melanogenesis was observed in human melanocytes HMV-II and HMV-II cell-containing 3D skin equivalents, but markedly prevented by ULH-002 as demonstrated by Fontana–Masson’s staining. In conclusion, our data suggested that ULH-002 could protect human keratinocytes and fibroblasts from UVA-induced injuries, prevent the loss of type IV and I collagens, as well as reduce melanogenesis. ULH-002 might be developed as a skin care reagent in the cosmetic industry.

scholarly journals 2-O-β-d-glucopyranosyl- -ascorbic acid, a novel vitamin C derivative from Lycium barbarum, prevents oxidative stress

Redox Biology ◽  
2019 ◽  
Vol 24 ◽  
pp. 101173 ◽  
Author(s):  
Shen-Fei Wang ◽  
Xin Liu ◽  
Mo-Yu Ding ◽  
Shuangcheng Ma ◽  
Jing Zhao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Lycium Barbarum

Ascorbic acid does not affect large elastic artery compliance or central blood pressure in young and older men

2004 ◽  
Vol 286 (4) ◽  
pp. H1528-H1534 ◽  
Author(s):  
Iratxe Eskurza ◽  
Kevin D. Monahan ◽  
Jed A. Robinson ◽  
Douglas R. Seals
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Ascorbic Acid ◽  
Carotid Artery ◽  
Vitamin C ◽  
Older Men ◽  
Healthy Men ◽  
Arterial Blood ◽  
Elastic Artery ◽  
Carotid Artery Compliance

Large elastic artery compliance is reduced and arterial blood pressure (BP) is increased in the central (cardiothoracic) circulation with aging. Reactive oxygen species may tonically modulate central arterial compliance and BP in humans, and oxidative stress may contribute to adverse changes with aging. If so, antioxidant administration may have beneficial effects. Young (Y; 26 ± 1 yr, mean ± SE) and older (O; 63 ± 2 yr, mean ± SE) healthy men were studied at baseline and during acute (intravenous infusion; Y: n = 13, O: n = 12) and chronic (500 mg/day for 30 days; Y: n = 10, O: n = 10) administration of ascorbic acid (vitamin C). At baseline, peripheral (brachial artery) BP did not differ in the two groups, but carotid artery compliance was 43% lower (1.2 ± 0.1 vs. 2.1 ± 0.1 mm2/mmHg × 10–1, P < 0.01) and central (carotid) BP (systolic: 116 ± 5 vs. 101 ± 3 mmHg, P < 0.05, and pulse pressure: 43 ± 4 vs. 36 ± 3 mmHg, P = 0.16), carotid augmentation index (AIx; 27.8 ± 7.8 vs. –20.0 ± 6.6%, P < 0.001), and aortic pulse wave velocity (PWV; 950 ± 88 vs. 640 ± 38 cm/s, P < 0.01) were higher in the older men. Plasma ascorbic acid concentrations did not differ at baseline (Y: 71 ± 5 vs. O: 61 ± 7 μmol/l, P = 0.23), increased ( P < 0.001) to supraphysiological levels during infusion (Y: 1,240 ± 57 and O: 1,056 ± 83 μmol/l), and were slightly elevated ( P < 0.001 vs. baseline) with supplementation (Y: 96 ± 5 μmol/l vs. O: 85 ± 6). Neither ascorbic acid infusion nor supplementation affected peripheral BP, heart rate, carotid artery compliance, central BP, carotid AIx, or aortic PWV (all P > 0.26). These results indicate that the adverse changes in large elastic artery compliance and central BP with aging in healthy men are not 1) mediated by ascorbic acid-sensitive oxidative stress (infusion experiments) and 2) affected by short-term, moderate daily ascorbic acid (vitamin C) supplementation.

An ascorbic acid-enriched tomato genotype to fight UVA-induced oxidative stress in normal human keratinocytes

2016 ◽  
Vol 163 ◽  
pp. 284-289 ◽  
Author(s):  
Ganna Petruk ◽  
Assunta Raiola ◽  
Rita Del Giudice ◽  
Amalia Barone ◽  
Luigi Frusciante ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Normal Human ◽  
Tomato Genotype

The protective role of ascorbic acid (vitamin C) against chlorpyrifos-induced oxidative stress in Oreochromis niloticus

2011 ◽  
Vol 38 (3) ◽  
pp. 635-643 ◽  
Author(s):  
Ferbal Özkan ◽  
Suna Gül Gündüz ◽  
Mehmet Berköz ◽  
Arzu Özlüer Hunt ◽  
Serap Yalın
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Oreochromis Niloticus ◽  
Protective Role

scholarly journals Study of oxidative stress biomarkers in obese children

2018 ◽  
Vol 6 (10) ◽  
pp. 3335
Author(s):  
Avni Kanji Fariya ◽  
Bina F. Dias
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Childhood Obesity ◽  
Vitamin C ◽  
Low Grade ◽  
Obese Children ◽  
Oxidative Stress Biomarkers ◽  
Plasma Ascorbic Acid ◽  
Increased Risk

Background: The objective is to study the oxidative stress in obese and non-obese children by assessing the biomarkers of lipid peroxidation and antioxidant status, Malondialdehyde (MDA) and Ascorbic acid (vitamin C) respectively. Childhood obesity is a growing global epidemic that requires attention due to the burden placed on the healthcare system for children and adults. Consumption of fatty foods and a high sugar, fat diet, and no exercise qualify as the main reasons for obesity among children and adults. Childhood obesity is connected with an increased risk of various diseases such as diabetes, cardiovascular, stroke, certain types of cancer later in life, social problems and depression among youths. Obesity is also characterized by chronic low grade inflammation with permanently increased oxidative stress (OS). Over-expression of oxidative stress damages cellular structures together with under-production of anti-oxidant mechanisms, leading to the development of obesity-related complications.Methods: The study involved 25 obese children for Vitamin C, 20 obese children for Malondialdehyde (MDA) and 18 non obese children for both Vitamin C and MDA in the age group of 5-14 years, without any complications. This study was conducted at L.T.M.M College. Plasma Ascorbic Acid was estimated colorimetrically by using 2,6- dicholrophenol indophenol dye and similarly Malondialdehyde was estimated colorimetrically by MDA-TBA colored complex.Results: The study showed significantly higher values of MDA and lower level of Vitamin C in obese children as compared with non-obese children.Conclusions: The levels of lipid peroxidation marker Malondialdehyde (MDA) is higher and level of antioxidant marker Ascorbic Acid (Vitamin C) is lower in obese children as compared with non-obese children. Thereby increasing oxidative stress and hence the oxidative damage to cells.

Vitamin C and E combination modulates oxidative stress induced by X-ray in blood of smoker and nonsmoker radiology technicians

2009 ◽  
Vol 27 (7) ◽  
pp. 424-429 ◽  
Author(s):  
Mustafa Kayan ◽  
Mustafa Nazıroğlu ◽  
Ömer Çelik ◽  
Kadir Yalman ◽  
Halis Köylü
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
X Ray ◽  
Radiology Technicians
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