Real-Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone-Marrow Micrometastasis Development at the Cellular Level in Nude Mice

2016 ◽  
Vol 117 (11) ◽  
pp. 2533-2537 ◽  
Author(s):  
Shinji Miwa ◽  
Makoto Toneri ◽  
Kentaro Igarashi ◽  
Shuya Yano ◽  
Hiroaki Kimura ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Nude Mice ◽  
Cellular Level ◽  
Confocal Fluorescence ◽  
Bone Marrow Micrometastasis ◽  
Confocal Fluorescence Imaging

scholarly journals Synthesis of Fluorescent Polythiophene Dots

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Zhen Liu ◽  
Alaa Nahhas ◽  
Li Liu ◽  
Earl Ada ◽  
Xinyu Zhang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Hydrazine Hydrate ◽  
Cancer Cells ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Chemical Oxidation ◽  
Prostate Cancer Cells ◽  
One Step ◽  
Live Mouse

Ring-functionalized semiconducting polythiophene dots (Pdots) were synthesized rapidly and in one step by the hydrazine hydrate reduction of doped parent polythiophene, obtained by conventional chemical oxidation of thiophene monomer by FeCl3 in anhydrous acetonitrile. Dispersions of these Pdots display robust (pseudo) solvatochromism and solvatofluorism. Polythiophene Pdots exhibit significant cytotoxicity towards prostate cancer cells (expected) although when injected subcutaneously in vivo in live mouse, no toxicity is observed for 24 days when monitored in real time using fluorescence imaging.

scholarly journals In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells

2008 ◽  
Vol 105 (10) ◽  
pp. 4050-4055 ◽  
Author(s):  
W. F. J. Vermaas ◽  
J. A. Timlin ◽  
H. D. T. Jones ◽  
M. B. Sinclair ◽  
L. T. Nieman ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging

scholarly journals Non-Invasive Confocal Fluorescence Imaging of Mice Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors

2021 ◽  
Author(s):  
Feifei Wang ◽  
Fuqiang Ren ◽  
Zhuoran Ma ◽  
Liangqiong Qu ◽  
Ronan Gourgues ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Fluorescence Imaging ◽  
Single Photon ◽  
Photon Detectors ◽  
Non Invasive ◽  
Single Photon Detectors ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Superconducting Nanowire

Light scattering by biological tissues sets a limit to the penetration depth of high-resolution optical microscopy imaging of live mammals in vivo. An effective approach to reduce light scattering and increase imaging depth is by extending the excitation and emission wavelengths to the > 1000 nm second near-infrared (NIR-II), also called the short-wavelength infrared (SWIR) window. Here, we developed biocompatible core-shell lead sulfide/cadmium sulfide (PbS/CdS) quantum dots emitting at ~1880 nm and superconducting nanowire single photon detectors (SNSPD) for single-photon detection up to 2000 nm, enabling one-photon fluorescence imaging window in the 1700-2000 nm (NIR-IIc) range. Confocal fluorescence imaging in NIR-IIc reached an imaging depth of ~ 800 μm through intact mouse head, and enabled non-invasive imaging of inguinal lymph nodes (LNs) without any surgery. In vivo molecular imaging of high endothelial venules (HEVs) with diameter down to ~ 6.6 μm in the lymph nodes was achieved, opening the possibility of non-invasive imaging of immune trafficking in lymph nodes at the single-cell/vessel level longitudinally.

scholarly journals In vivo confocal fluorescence imaging of skin surface cellular morphology

2002 ◽  
Vol 8 (S02) ◽  
pp. 1062-1063
Author(s):  
Daniel Thorn Leeson ◽  
C. Lynn Meyers ◽  
Kumar Subramanyan
Keyword(s):  
Fluorescence Imaging ◽  
Skin Surface ◽  
Cellular Morphology ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging
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