Chondroitin Sulfate-E Binds to Both Osteoactivin and Integrin αVβ3 and Inhibits Osteoclast Differentiation

2015 ◽  
Vol 116 (10) ◽  
pp. 2247-2257 ◽  
Author(s):  
Tatsuya Miyazaki ◽  
Satoshi Miyauchi ◽  
Takahisa Anada ◽  
Akira Tawada ◽  
Osamu Suzuki
Keyword(s):  
Chondroitin Sulfate ◽  
Osteoclast Differentiation ◽  
Integrin Αvβ3 ◽  
Chondroitin Sulfate E

Chondroitin sulfate-e enhances osteoblast differentiation and inhibits osteoclast differentiation in vitro

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S314
Author(s):  
T. Miyazaki ◽  
S. Miyauchi ◽  
A. Tawada ◽  
T. Anada ◽  
S. Matsuzaka ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Osteoblast Differentiation ◽  
Osteoclast Differentiation ◽  
Chondroitin Sulfate E

scholarly journals Effect of chondroitin sulfate-E on the osteoclastic differentiation of RAW264 cells

2010 ◽  
Vol 29 (4) ◽  
pp. 403-410 ◽  
Author(s):  
Tatsuya MIYAZAKI ◽  
Satoshi MIYAUCHI ◽  
Akira TAWADA ◽  
Takahisa ANADA ◽  
Osamu SUZUKI
Keyword(s):  
Chondroitin Sulfate ◽  
Chondroitin Sulfate E ◽  
Osteoclastic Differentiation

Oversulfated chondroitin sulfate-E binds to BMP-4 and enhances osteoblast differentiation

2008 ◽  
Vol 217 (3) ◽  
pp. 769-777 ◽  
Author(s):  
Tatsuya Miyazaki ◽  
Satoshi Miyauchi ◽  
Akira Tawada ◽  
Takahisa Anada ◽  
Satoshi Matsuzaka ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Osteoblast Differentiation ◽  
Chondroitin Sulfate E ◽  
Oversulfated Chondroitin Sulfate

scholarly journals In vitro activation of the contact (Hageman factor) system of plasma by heparin and chondroitin sulfate E

Blood ◽  
1984 ◽  
Vol 63 (6) ◽  
pp. 1453-1459 ◽  
Author(s):  
Y Hojima ◽  
CG Cochrane ◽  
RC Wiggins ◽  
KF Austen ◽  
RL Stevens
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Connective Tissue ◽  
Chondroitin Sulfate ◽  
Mouse Bone Marrow ◽  
Hageman Factor ◽  
In Vitro Activation ◽  
Chondroitin Sulfate E ◽  
Charged Macromolecules

Abstract A large number of negatively charged macromolecules, including DNA, glycosaminoglycans, and proteoglycans, were tested as possible activators of the contact (Hageman factor) system in vitro. Activation was assessed by conversion of prekallikrein to kallikrein, as determined by amidolytic assay and by cleavage of 125I-Hageman factor into 52,000- and 28,000-dalton fragments. Of particular interest to these studies, heparin proteoglycan and glycosaminoglycan from rat peritoneal mast cells, and squid chondroitin sulfate E, which is representative of the glycosaminoglycan from cultured mouse bone marrow derived mast cells, induced the reciprocal activation between Hageman factor and prekallikrein. In addition, naturally occurring heparin glycosaminoglycans from pig mucosa, bovine lung, and rat mast cells also induced activation. In contrast, native connective tissue matrix glycosaminoglycans and proteoglycans from several sources were inactive, although when one such chondroitin sulfate was further sulfated in vitro, it gained activity. When the negative charge of the activating agents was blocked by the addition of hexadimethrine bromide, the cleavage of 125I-Hageman factor in the presence of prekallikrein was prevented. The active negatively charged macromolecules induced cleavage of 125I-high molecular weight kininogen in normal plasma but not in Hageman factor-deficient or prekallikrein- deficient plasmas. Reconstitution of prekallikrein-deficient plasma with purified prekallikrein restored the kininogen cleavage upon addition of the active proteoglycans. These results suggest that both heparin from connective tissue mast cells and highly sulfated chondroitin sulfate E from cultured mouse bone marrow derived mast cells (which are considered synonomous with mucosal mast cells) could activate the contact system of plasma subsequent to an activation secretion response.

The synthesis and biological evaluation of chondroitin sulfate E glycodendrimers

2019 ◽  
Vol 11 (12) ◽  
pp. 1403-1415 ◽  
Author(s):  
Shuang Yang ◽  
Haijing Zhang ◽  
Qifeng Liu ◽  
Shanshan Sun ◽  
Pingsheng Lei ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Biological Evaluation ◽  
Chondroitin Sulfate E ◽  
Synthesis And Biological Evaluation

scholarly journals Contactin-1 Is a Functional Receptor for Neuroregulatory Chondroitin Sulfate-E

2008 ◽  
Vol 284 (7) ◽  
pp. 4494-4499 ◽  
Author(s):  
Tadahisa Mikami ◽  
Daiki Yasunaga ◽  
Hiroshi Kitagawa
Keyword(s):  
Chondroitin Sulfate ◽  
Chondroitin Sulfate E ◽  
Functional Receptor

Chondroitin sulfate E blocks enzymatic action of heparanase and heparanase-induced cellular responses

2019 ◽  
Vol 520 (1) ◽  
pp. 152-158
Author(s):  
Nobuaki Higashi ◽  
Rino Maeda ◽  
Nakaba Sesoko ◽  
Momoko Isono ◽  
Sodai Ishikawa ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Cellular Responses ◽  
Enzymatic Action ◽  
Chondroitin Sulfate E
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